Molecular cloning and characterization of novel developmentally-regulated genes

新型发育调控基因的分子克隆和表征

• 批准号: 06680778
• 负责人: SAITO Yumiko
• 金额: $ 1.54万
• 依托单位: Tokyo Metropolitan Institute of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680778/
• 关键词: Neural differentiation; Gene; Splicing; Subtraction; Brain; Neuropeptide

We used a subtraction cloning approach to isolate cDNAs of N23K and N27K, whose mRNA and protein are transiently up-regulated in mouse NS20Y cells undergoing neurite outgrowth induced by treatment of dibutyryl cyclic AMP.N23K and N27K encode precursor proteins for a newly discovered rat neuropeptide nociceptin. As with other neuropeptide precursors, the N23K/N27K proteins contain a putative signal peptide rich in hydrophobic amino acids at the N-terminus. N23K/N27K mRNA are present only in brain and spinal cord in adult mouse, and are expressed at higher levels in early postnatal brain. These findings suggest that the N23K/N27K protein function not only as neuropeptide precursors but also as important components in neuronal differentiation.

本研究采用差减克隆的方法，克隆了双丁酰环腺苷酸（dibutyryl cyclicAMP，DcAMP）诱导的小鼠神经元突起生长过程中，N23 K和N27 K的mRNA和蛋白质表达均瞬时上调的cDNAs，它们编码新发现的大鼠神经肽nociceptin的前体蛋白。与其他神经肽前体一样，N23 K/N27 K蛋白在N-末端含有富含疏水氨基酸的推定信号肽。N23 K/N27 K mRNA仅存在于成年小鼠的脑和脊髓中，并且在出生后早期的脑中以较高水平表达。这些结果表明，N23 K/N27 K蛋白不仅作为神经肽前体，而且作为神经元分化的重要组成部分的功能。

项目成果

Yumiko Saito et al.: "N23K,a gene transiently up-regulated during neural differentiation, encodes a precursor protein for a newly identified neuropeptide nociceptin" Biochem Biophys Res Commun.217. 539-545 (1995)

Yumiko Saito 等人：“N23K 是一种在神经分化过程中短暂上调的基因，编码新鉴定的神经肽伤害感受肽的前体蛋白”Biochem Biophys Res Commun.217。

Yumiko SAITO: "The calpain-calpastatin system is regnlated differently clning hnman nenroblastoma cell differentiation to Schnannian and nerronal cells" FEBS Letters. 353. 327-331 (1994)

Yumiko SAITO：“钙蛋白酶-钙蛋白酶抑制素系统在人类神经母细胞瘤细胞分化为施南细胞和神经元细胞时受到不同的调节”FEBS Letters。

Yumiko Saito. et. al: "N23K, a gene transiontly up-regulated dnling neural differentiation, encodes a precursor protein for a newly idontified nenropoptide nocicaptin" Biochem. Biophys. Res. Commnn. 217. 539-545 (1995)

齐藤由美子。

Yumiko Saito et al.: "N23K, a gone transionthy up-regnlated dnling nenral differentiation, encodes a precursor protein for anewly identified nenropeptide nociceptin" Biochem. Biophys. Res. Commnn.217. 539-545 (1995)

Yumiko Saito 等人：“N23K 是一种上调的神经元神经分化，编码新鉴定的神经肽伤害感受素的前体蛋白”Biochem。