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Significance of detection of drug-resistant organisms in laboratory animal colonies

实验动物群体耐药菌检测的意义

基本信息

批准号：
06680837
负责人：
MAEJIMA Kazuyoshi
金额：
$ 1.34万
依托单位：
Keio University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680837/
关键词：
Drug-resistant Laboratory animals Intestinal flora E.coli Streptococcus Staphylococcus MRSA 院内感染モデル

项目摘要

According to our previous data obtained in 1960s and 1980s in Japan, drug-resistant organisms were temporarily isolated from the laboratory animals given antibiotics for control of infectious diseases, and were hardly detected in the animals untreated with antibiotics. These observations were apparently different from the findings that various types of drug-resistant organisms were widely contaminated in human beings and domestic animals. Based on these data, we have a plan to establish an animal model for investigation on the distribution of drug resistant organisms in human and animal wards.At first, the present examination was made to confirm our previous data that drug-resistant organisms were hardly isolated from the laboratory animals. Isolation of E.coli, Streptococcus faecalis and Staphylococcus aureus was made on the fresh feces of mice, rats, guinea-pigs and rabbits housed in 5 commercial breeders and 5 animal experimentation facilities in Kyushu aereas. Using a routine techniques, the isolates were examined for drug-resistance to 9 antibiotics including ampicillin, tetracyclin, streptomycin, kanamycin, gentamycin and others.All the isolates were resistant to various antibiotics with different paterns. Drug-resistant organisms were also isolated from laboratory animals though given no antibiotics. The present results indicated that drug-resistant organisms were widely disturbed in the laboratory animals same as human beings and domestic animals. And now, laboratory animals seems to be not an adequate model for investigation on the distribution of drug-resistant oragnaims in the wards.

根据我们在20世纪60年代和80年代在日本获得的数据，耐药微生物暂时从给予抗生素以控制传染病的实验动物中分离出来，在未使用抗生素的动物中几乎检测不到。这些观察结果显然不同于在人和家畜中广泛污染各种类型的耐药生物的发现。基于这些数据，我们计划建立一种动物模型，用于研究耐药菌在人和动物病房中的分布。首先，进行本研究以证实我们以前的数据，即耐药菌几乎不从实验动物中分离。从九州地区5个商业饲养场和5个动物实验设施中饲养的小鼠、大鼠、豚鼠和家兔的新鲜粪便中分离出大肠杆菌、粪链球菌和金黄色葡萄球菌。用常规方法检测了分离菌株对氨苄西林、四环素、链霉素、卡那霉素、庆大霉素等9种抗生素的耐药性，结果表明，分离菌株对多种抗生素耐药，耐药程度不同。尽管没有给予抗生素，但也从实验室动物中分离出了耐药性微生物。结果表明，耐药菌在实验动物中与人、家畜一样普遍存在。目前，实验室动物似乎不适合作为研究耐药菌在病房中分布的模型。