Neurotrophin and their receptor mRNA expressions in neurodegenerative diseases.

神经营养蛋白及其受体 mRNA 在神经退行性疾病中的表达。

• 批准号: 06807059
• 负责人: SOBUE Gen
• 金额: $ 0.83万
• 依托单位: Nagoya University (1995)Aichi Medical University (1994)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06807059/
• 关键词: Neuro degenerative disease; Neuro trophic factor; Neuronal cell death; mRNA; 末梢神経障害; neurotrophin; trk-family

Neurotrophin (NGF,BDNF,NT-3, GDNF) mRNA and their ceceptor (p75NGFR,trkA,trkB,and trkC) mRNA expressions in the neural tissues (brain, spinal cord, dorsal root ganglion, sympathetic ganglion, spinal nerve root, peripheral nerve trunk) and general visceral tissues (kidney, heart, spleen, liver, testis, muscle and intestine) from normal human adults and patients with amyotrophic lateral sclerosis (ALS) was assessed by the quantitative RT-PCR.In the normal subjects, NGF,BDNF,trkA and trkB mRNAs were expressed mainly in the neural tissues, but NT-3 and trkC mRNAs were expressed through more wide ranged tissues including general visceral organs. GDNF mRNA was rather exclusively expressed in the muscle and spinal cord. In the tissues from the ALS patients, GDNF mRNA levels were increased in the spinal cord and decreased in muscles.

用定量RT-PCRNAs方法检测了正常成人和肌萎缩侧索硬化症(ALS)患者的神经组织(脑、脊髓、背根神经节、交感神经节、脊神经根、周围神经干)和全身内脏组织(肾、心、脾、肝、睾丸、肌肉和肠)中神经营养因子(NGF、BDNF、NT-3、GDNF)及其受体(p75NGFR、TrkA、TrkB和trkC)的mRNA表达。在正常人中，NGF、BDNF、TrkA和TrkB的mRNAs主要在神经组织中表达。但NT-3和trkC mRNAs在更广泛的组织中表达，包括一般内脏器官。GDNF mRNA在肌肉和脊髓中有较强的特异性表达。在ALS患者的组织中，GDNF在脊髓中的表达增加，在肌肉中的表达降低。

项目成果

Shimada N,Sobue G,Doyu M,Yamamoto K,Yasuda T,Mukai E,Kachi T,Mitsuma T:"X-linked recessive bulbospinal neuronopathy:clinical phenotypes and CAG repeat size in androgen receptor gene." Muscle&Nerve. 18. 1378-1384 (1995)

Shimada N、Sobue G、Doyu M、Yamamoto K、Yasuda T、Mukai E、Kachi T、Mitsuma T：“X 连锁隐性球脊髓神经元病：雄激素受体基因中的临床表型和 CAG 重复大小。”

Sobue G,Doyu M,Nakao N,Shimada N,Mitsuma T,Maruyama H,Kawakami H,Nakamura S:"Homozygosity for Machado-Joseph disease gene enhances phenotypic severity." J Neurol Neurosurg Psy. (in press). (1996)

Sobue G，Doyu M，Nakao N，Shimada N，Mitsuma T，Maruyama H，Kawakami H，Nakamura S：“Machado-Joseph 病基因的纯合性增强了表型严重程度。”

Tanaka F.Sobue G.Doyu M,Ito Y,Yamamoto M,Shimada N,Yamamoto K,Riku S,Hashizume Y,Mitsuma T:"Differential pattern in tissue-specific somatic mosaicism of expanded CAG trinucleotide repeat in dentatorubral-pallidoluysian atrophy.Machado-Joseph disease,and X

Tanaka F.Sobue G.Doyu M、Ito Y、Yamamoto M、Shimada N、Yamamoto K、Riku S、Hashizume Y、Mitsuma T：“齿状红核-苍白球路易体萎缩中扩展的 CAG 三核苷酸重复的组织特异性体细胞镶嵌的差异模式。

Mei Li, Gen Sobue, Manabu Doyu, Eiichiro Mukai, Yoshio Hashizume, Terunori Mitsuma: "Primary sensory neurons in Xlinked recessive bulbospinal neuronopathy : histopathology and androgen receptor gene ezpression." Muscle & Nerve. 18. 301-308 (1995)

Mei Li、Gen Sobue、Manabu Doyu、Eiichiro Mukai、Yoshio Hashizume、Terunori Mitsuma：“X连锁隐性球脊髓神经元病中的初级感觉神经元：组织病理学和雄激素受体基因表达。”

Shimada N,Sobue G,Doyu M,Yamamoto K,Yasuda T,Mukai E,Kachi T,Mitsuma T:"X-linked recessive bulbospinal neuronopathy:clinical phenotypes and CAG repeat size in androgen receptor gene." Muscle & Nerve. 18. 1378-1384 (1995)

Shimada N、Sobue G、Doyu M、Yamamoto K、Yasuda T、Mukai E、Kachi T、Mitsuma T：“X 连锁隐性球脊髓神经元病：雄激素受体基因中的临床表型和 CAG 重复大小。”