Neurotrophin and their receptor mRNA expressions in neurodegenerative diseases.

神经营养蛋白及其受体 mRNA 在神经退行性疾病中的表达。

• 批准号: 06807059
• 负责人: SOBUE Gen
• 金额: $ 0.83万
• 依托单位: Nagoya University (1995)Aichi Medical University (1994)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06807059/
• 关键词: Neuro degenerative disease; Neuro trophic factor; Neuronal cell death; mRNA; 末梢神経障害; neurotrophin; trk-family

Neurotrophin (NGF,BDNF,NT-3, GDNF) mRNA and their ceceptor (p75NGFR,trkA,trkB,and trkC) mRNA expressions in the neural tissues (brain, spinal cord, dorsal root ganglion, sympathetic ganglion, spinal nerve root, peripheral nerve trunk) and general visceral tissues (kidney, heart, spleen, liver, testis, muscle and intestine) from normal human adults and patients with amyotrophic lateral sclerosis (ALS) was assessed by the quantitative RT-PCR.In the normal subjects, NGF,BDNF,trkA and trkB mRNAs were expressed mainly in the neural tissues, but NT-3 and trkC mRNAs were expressed through more wide ranged tissues including general visceral organs. GDNF mRNA was rather exclusively expressed in the muscle and spinal cord. In the tissues from the ALS patients, GDNF mRNA levels were increased in the spinal cord and decreased in muscles.

神经营养（NGF，BDNF，NT-3，GDNF）mRNA及其受体（p75 NGFR、trkA、trkB和trkC）mRNA在神经组织中的表达（脑、脊髓、背根神经节、交感神经节、脊神经根、周围神经干）和一般内脏组织（肾、心脏、脾、肝、睾丸，通过定量RT-PCR评估正常成人和肌萎缩侧索硬化症（ALS）患者的NGF，BDNF，trkA和trkB mRNA主要在神经组织中表达，而NT-3和trkC mRNA在包括一般内脏器官在内的更广泛组织中表达。GDNF mRNA仅在肌肉和脊髓中表达。在ALS患者的组织中，GDNF mRNA水平在脊髓中增加，在肌肉中减少。

项目成果

Shimada N,Sobue G,Doyu M,Yamamoto K,Yasuda T,Mukai E,Kachi T,Mitsuma T:"X-linked recessive bulbospinal neuronopathy:clinical phenotypes and CAG repeat size in androgen receptor gene." Muscle&Nerve. 18. 1378-1384 (1995)

Shimada N、Sobue G、Doyu M、Yamamoto K、Yasuda T、Mukai E、Kachi T、Mitsuma T：“X 连锁隐性球脊髓神经元病：雄激素受体基因中的临床表型和 CAG 重复大小。”

Sobue G,Doyu M,Nakao N,Shimada N,Mitsuma T,Maruyama H,Kawakami H,Nakamura S:"Homozygosity for Machado-Joseph disease gene enhances phenotypic severity." J Neurol Neurosurg Psy. (in press). (1996)

Sobue G，Doyu M，Nakao N，Shimada N，Mitsuma T，Maruyama H，Kawakami H，Nakamura S：“Machado-Joseph 病基因的纯合性增强了表型严重程度。”

Tanaka F.Sobue G.Doyu M,Ito Y,Yamamoto M,Shimada N,Yamamoto K,Riku S,Hashizume Y,Mitsuma T:"Differential pattern in tissue-specific somatic mosaicism of expanded CAG trinucleotide repeat in dentatorubral-pallidoluysian atrophy.Machado-Joseph disease,and X

Tanaka F.Sobue G.Doyu M、Ito Y、Yamamoto M、Shimada N、Yamamoto K、Riku S、Hashizume Y、Mitsuma T：“齿状红核-苍白球路易体萎缩中扩展的 CAG 三核苷酸重复的组织特异性体细胞镶嵌的差异模式。

Mei Li, Gen Sobue, Manabu Doyu, Eiichiro Mukai, Yoshio Hashizume, Terunori Mitsuma: "Primary sensory neurons in Xlinked recessive bulbospinal neuronopathy : histopathology and androgen receptor gene ezpression." Muscle & Nerve. 18. 301-308 (1995)

Mei Li、Gen Sobue、Manabu Doyu、Eiichiro Mukai、Yoshio Hashizume、Terunori Mitsuma：“X连锁隐性球脊髓神经元病中的初级感觉神经元：组织病理学和雄激素受体基因表达。”

Shimada N,Sobue G,Doyu M,Yamamoto K,Yasuda T,Mukai E,Kachi T,Mitsuma T:"X-linked recessive bulbospinal neuronopathy:clinical phenotypes and CAG repeat size in androgen receptor gene." Muscle & Nerve. 18. 1378-1384 (1995)

Shimada N、Sobue G、Doyu M、Yamamoto K、Yasuda T、Mukai E、Kachi T、Mitsuma T：“X 连锁隐性球脊髓神经元病：雄激素受体基因中的临床表型和 CAG 重复大小。”