GENE EXPRESSION AND REGULATION OF NEUROTROPHINS AND THEIR RECEPTORS IN PERIPHERAL NEUROPATHIES

周围神经病中神经营养因子及其受体的基因表达和调节

基本信息

  • 批准号:
    02807085
  • 负责人:
  • 金额:
    $ 1.15万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1990
  • 资助国家:
    日本
  • 起止时间:
    1990 至 1992
  • 项目状态:
    已结题

项目摘要

Gene expression of the neurotrophic factors and their receptors and related molecules was analyzed in the human, rat and mouse peripheral nervous system, particularly in the process of the peripheral nerve degeneration and regeneration. The molecules analyzed in this study included NGF, BDNF, LNGFR, trk,trkB, laminin A, B_1, B_2 chains and collagen type I, III, IV. Expression levels of mRNA for these molecules in the tissues were determined by Northern blot analysis. The findings obtained are as follows :NGF, BDNF, LNGFR, laminin B_1, B_2, collagen type I, III, IV were synthesized in cultured Schwann cells inculture, and their mRNA levels were up- or down-regulated by exogenously administered cAMP analogues or by the contact with sensory neurons.Human DRG neurons and sympathetic neurons express LNGFR, trk, trkBmRNAs, and which were likely down-regulated in the cases with neuropathy particularly in axonal type.LNGFR were up- regulated and laminin B_1, B_2 were down-reglated in the axonal nerve lesions of humans as well as mouse and rats, but which were reversely reglated in associated with nerve regenerationNeurite arborization (sprouting) was significantly enhanced by NGF on the post-matured DRG neurons, and this ability was well retained in the aging process, which may have corresponded to the well preserved functional NGF receptor (trk) expression on the aged these neurous. Those finding confirmed that these neurotrophic molecules and their receptors are up-or down-regulated in association with peripheral nerve de-and regeneration suggesting their significant roles in the neuropathies. Further study on the signal mechanism for these gene expressions is now required.
分析了人、大鼠和小鼠周围神经系统,特别是周围神经变性和再生过程中神经营养因子及其受体及相关分子的基因表达。本研究分析的分子包括NGF、BDNF、lnfr、trk、trkB、层粘连蛋白A、B_1、B_2链和I型、III型、IV型胶原,通过Northern blot检测这些分子在组织中的mRNA表达水平。结果表明:在培养的雪旺细胞中可合成NGF、BDNF、lnfr、层粘连蛋白B_1、B_2、I型、III型、IV型胶原,外源性cAMP类似物或与感觉神经元接触可上调或下调其mRNA水平。人类DRG神经元和交感神经元表达lnfr、trk、trkbmrna,这些mrna在神经病变特别是轴突型患者中可能下调。NGF在人、小鼠和大鼠轴突神经损伤中表达lnfr上调,层粘连蛋白B_1、B_2下调,但与神经再生相关。NGF在成熟后DRG神经元上显著增强了神经突的生长(出芽)能力,且这种能力在衰老过程中保持良好,这可能与NGF受体(trk)在衰老后这些神经元上的功能表达保持良好有关。这些发现证实了这些神经营养分子及其受体的上调或下调与周围神经的去和再生有关,表明它们在神经病变中起着重要作用。这些基因表达的信号机制有待进一步研究。

项目成果

期刊论文数量(44)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
祖父江 元:"末梢神経の再生とその調分因子:low affinity NGF receptor と laminin" 末梢神経. 2. 37-43 (1991)
Gen Sobue:“周围神经再生及其调节因素:低亲和力 NGF 受体和层粘连蛋白”《周围神经》2. 37-43 (1991)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Sobue G, Nakao N, Murakami K, Yasuda T, Sahashi K, Sasaki Y, Sakaki Y, Mitsuma T, Takahashi A: "Type I familial amyloid polyneuropathy : A pathological study of the peripheral nervous system" Brain. 113. 903-919 (1990)
Sobue G、Nakao N、Murakami K、Yasuda T、Sahashi K、Sasaki Y、Sakaki Y、Mitsuma T、Takahashi A:“I 型家族性淀粉样多发性神经病:周围神经系统的病理学研究”脑。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yamamoto M,Sobue G,Li Mei,Arakawa Y,Mitsuma T,Kimata K:"cAMP-dependent regulation of nerve growth factor(NGF),brain-dirived neurotrophic factor(BDNF)and low-affinity nerve growth factor receptor(LNGFR)mRNA levels in cultured rat Schwann cells." Neurosci L
Yamamoto M,Sobue G,Li Mei,Arakawa Y,Mitsuma T,Kimata K:“神经生长因子(NGF),脑源性神经营养因子(BDNF)和低亲和力神经生长因子受体(LNGFR)的cAMP依赖性调节
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
YASUDA T,SOBUE G,ITO T,MITSUMA T,TAKAHASHI A:"Nerve growth factor enhances neurite arborization of the adult sensory neurons:A study in single-neuron culture." Brain Res. 524. 54-63 (1990)
YASUDA T、Sobue G、ITO T、MITSUMA T、TAKAHASHI A:“神经生长因子增强成人感觉神经元的神经突树枝化:单神经元培养的研究。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
KEN E,SOBUE G,YASUDA T,ITO T,MITSUMA T:"Effects of nerve growth factor on the geometry of adult rat sensory neurons." J Aichi Med Univ Ass. 18. 457-463 (1990)
KEN E、Sobue G、YASUDA T、ITO T、MITSUMA T:“神经生长因子对成年大鼠感觉神经元几何形状的影响。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

SOBUE Gen其他文献

SOBUE Gen的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('SOBUE Gen', 18)}}的其他基金

Therapeutic strategy of neurodegenerative disease using microRNA
利用microRNA治疗神经退行性疾病的策略
  • 批准号:
    25670418
  • 财政年份:
    2013
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Identification of therapeutic targets for ALS by screening for TDP-43 and FUS associated molecules
通过筛选 TDP-43 和 FUS 相关分子鉴定 ALS 治疗靶点
  • 批准号:
    23659452
  • 财政年份:
    2011
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Development of Molecular Targeted Disease Modifying Therapy for Polyglutamine Diseases
多聚谷氨酰胺疾病分子靶向疾病修饰疗法的发展
  • 批准号:
    21229011
  • 财政年份:
    2009
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (S)
Pathogenesis-based therapy development for polyglutamine diseases
多聚谷氨酰胺疾病的基于发病机制的治疗方法开发
  • 批准号:
    19209033
  • 财政年份:
    2007
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Development of pathogenesis-based therapy for polyglutamine diseases
多聚谷氨酰胺疾病的基于发病机制的治疗方法的开发
  • 批准号:
    17025020
  • 财政年份:
    2005
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Treatment of polyglutamine disease using small molecular compound
小分子化合物治疗多聚谷氨酰胺病
  • 批准号:
    17209032
  • 财政年份:
    2005
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Development of Pathogenesis-based treatment for spinal and bulbar muscular atrophy
脊髓和延髓性肌萎缩症基于发病机制的治疗方法的开发
  • 批准号:
    15209031
  • 财政年份:
    2003
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Elucidation of molecular mechanism of neuronal cell death in spinal and bulbar muscular atrophy
阐明脊髓和延髓肌萎缩症中神经元细胞死亡的分子机制
  • 批准号:
    12210010
  • 财政年份:
    2000
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Neurotrophin and their receptor mRNA expressions in neurodegenerative diseases.
神经营养蛋白及其受体 mRNA 在神经退行性疾病中的表达。
  • 批准号:
    06807059
  • 财政年份:
    1994
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

Targeting axonal transport regulation by neurotrophic factors to treat peripheral nerve degeneration
通过神经营养因子调节轴突运输来治疗周围神经变性
  • 批准号:
    MR/Y010949/1
  • 财政年份:
    2024
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Fellowship
Hierarchically-Structured Conduits with Programmed Release of Neurotrophic Factors for Repairing Large Defects in Thick Nerves
具有程序化释放神经营养因子的分层结构导管用于修复粗神经的大缺损
  • 批准号:
    10579569
  • 财政年份:
    2023
  • 资助金额:
    $ 1.15万
  • 项目类别:
Peripheral Neurotrophic Factors in the Regulation of Adipose Tissue Energy Expenditure
周围神经营养因子调节脂肪组织能量消耗
  • 批准号:
    10323153
  • 财政年份:
    2021
  • 资助金额:
    $ 1.15万
  • 项目类别:
Development of BBB-passable polymeric micelles for sustained expression of neurotrophic factors in the brain
开发可通过血脑屏障的聚合物胶束,用于大脑中神经营养因子的持续表达
  • 批准号:
    21K19888
  • 财政年份:
    2021
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Challenging Research (Exploratory)
Mechanism of promoting wound healing by the coordinated action of growth factors and neurotrophic factors
生长因子与神经营养因子协同作用促进伤口愈合的机制
  • 批准号:
    19K07319
  • 财政年份:
    2019
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Regulation of axonal transport by neurotrophic factors in health and disease
健康和疾病中神经营养因子对轴突运输的调节
  • 批准号:
    MR/S006990/1
  • 财政年份:
    2019
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Fellowship
JPND LOss of neurotrophic factors in neurodegenerative DEmentias: Back to the crossroads of proteins (LODE)
JPND 神经退行性痴呆中神经营养因子的丢失:回到蛋白质的十字路口 (LODE)
  • 批准号:
    MR/R02488X/1
  • 财政年份:
    2018
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Research Grant
Peripheral Neurotrophic Factors in the Regulation of Adipose Tissue Energy Expenditure
周围神经营养因子调节脂肪组织能量消耗
  • 批准号:
    9522965
  • 财政年份:
    2018
  • 资助金额:
    $ 1.15万
  • 项目类别:
Examining the neuroprotective effects of physical activity on hippocampal function in older adults through potential mechanisms related to inflammation and neurotrophic factors
通过与炎症和神经营养因子相关的潜在机制检查体力活动对老年人海马功能的神经保护作用
  • 批准号:
    528549-2018
  • 财政年份:
    2018
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Alexander Graham Bell Canada Graduate Scholarships - Master's
Prevention and treatment of postoperative cognitive dysfunction by regulating the expression of neurotrophic factors
调节神经营养因子表达防治术后认知功能障碍
  • 批准号:
    17K11080
  • 财政年份:
    2017
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了