Chromatin Remodeling and Epigenetics

染色质重塑和表观遗传学

• 批准号: 14208086
• 负责人: HIROSE Susumu
• 金额: $ 33.95万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14208086/
• 关键词: Chromatin Remodling; Epigenetics; Drosophila; GAGA factor; FACT; Brahma Complex; Position Effect Variegation; Supercoiling Factor; Position effect variegation; 活性クロマチン; 不活性クロマチン; SSRP1; SPT16; Hox遺伝子

1) We elucidated the mechanism underlying the site-specific chromatin remodeling around the GAGA factor-binding site. First, GAGA factor recruits FACT to its binding site. Then, FACT induces a conformational change in the neighboring nucleosomes, which in turn facilitates chromatin remodeling.2) We identified subunits of the Brahma chromatin remodeling compex as the GAGA factor-associated proteins besides FACT, suggesting that Brahma complex is involved in the GAGA factor-FACT complex induced chromatin remodeling.3) Genetic analysis revealed that the GAGA factor-FACT complex contributes to epigenetic maintenance of Hox gene expression.4) Genetic and biochemical analyses demonstrated that the GAGA factor-FACT complex is involved in position effect variegation.5) We found a crucial role of Drosophila supercoiling factor in the dosage compensation of X chromosome using RNAi in vivo.

1)我们阐明了GAGA因子结合位点周围的位点特异性染色质重塑的机制。首先，GAGA因子将FACT募集到其结合位点。然后，FACT诱导邻近核小体的构象变化，这反过来又促进染色质重塑。2）我们鉴定了Brahma染色质重塑复合体的亚基作为FACT以外的GAGA因子相关蛋白，提示Brahma复合物参与GAGA因子-FACT复合物诱导的染色质重塑。3）遗传分析显示GAGA因子-FACT复合物参与了Hox基因表达的表观遗传维持; 4）遗传学和生化分析表明GAGA因子-FACT复合物参与了位置效应变异; 5）果蝇超螺旋因子在体内RNAi对X染色体的剂量补偿中起重要作用。

项目成果

Drosophila MBF1 is a co-activator for Tracheae Defective and contributes to the formation of tracheal and nervous systems

• DOI: 10.1242/dev.00297
• 发表时间: 2003-02-01
• 期刊: DEVELOPMENT
• 影响因子: 4.6
• 作者: Liu, QX;Jindra, M;Hirose, S
• 通讯作者: Hirose, S

Three Arabidopsis MBF1 homologs with distinct expression profiles play roles as transcriptional co-activators.

• DOI: 10.1093/pcp/pch017
• 发表时间: 2004-02
• 期刊: Plant & cell physiology
• 影响因子: 4.9
• 作者: K. Tsuda;T. Tsuji;S. Hirose;K. Yamazaki

Cloning of Cyc (Bmal-1) homolog in Bombyx mori ; Structural analyses and the tissue specific distributions.

家蚕中 Cyc (Bmal-1) 同源物的克隆；

• 发表时间: 2003
• 期刊: Comp. Biochem. Physiol. B 10
• 作者: Markova;E. P.
• 通讯作者: E. P.

Shimojima, T.: "Drosophila FACT contributes to Hox gene expression through physical and functional interaction with GAGA factor"Genes & Development. (印刷中). (2003)

Shimojima, T.：“果蝇 FACT 通过与 GAGA 因子的物理和功能相互作用促进 Hox 基因表达”《基因与发展》（出版中）。

Liu, Q.-X.: "Drosophila MBF1 is a co-activator for Tracheae Defective and contributes to the formation of tracheal and nervous systems"Development. 130. 719-728 (2003)

Liu，Q.-X.：“果蝇MBF1是气管缺陷的共激活剂，有助于气管和神经系统的形成”的开发。