Chromatin dynamics underlying cellular memory

细胞记忆背后的染色质动力学

• 批准号: 17002018
• 负责人: HIROSE Susumu
• 金额: $ 144.52万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Specially Promoted Research
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17002018/
• 关键词: Cellular memory; Chromatin; Drosophila; Mouse; GAGA factor; FACT; Supercoling factor; non-coding RNA

We have obtained following results through this study.1. GAGA factor FACT complex recruits chromatin remodeling complex PBAP to Pc/trx response elements, induces chromatin remodeling, and maintains expression of Hox genes.2. GAGA factor-FACT complex binds to a GA repeat sequence downstream the white gene, induces chromatin remodeling, replaces histone H3 by its variant H3.3, and maintains white gene expression by counteracting the heterochromatin spreading.3. Transcription of non-coding RNA through Pc/trx response element regulates Hox gene expression.4. Supercoiling factor is recruited to chromatin acetylated at histone H4 K16 by MOF, and contributes dosage compensation of X chromosome through counteracting with ISWI.5. Ash1 knock-out mice are homozygous viable but show homeotic transformation of the back-one. SPT16 knock-out mice are embryonic lethal in homozygotes. Its heterozygotes show a deviation in the levels of IgG sublasses.

通过本研究，我们取得了以下研究成果。结论1.GAGA因子-事实复合体招募染色质重塑复合体PBAP到Pc/Trx反应元件，诱导染色质重塑，维持HOX基因的表达。GagA因子-事实复合体与白色基因下游的GA重复序列结合，诱导染色质重塑，用其变异体H3.3取代组蛋白H3，并通过抵消异染色质的扩散来维持白色基因的表达。非编码RNA通过Pc/Trx反应元件转录调控HOX基因的表达。超卷曲因子被MOF补充到组蛋白H4 K16上的染色质乙酰化，并通过与ISWI的相互作用参与X染色体的剂量补偿。Ash1基因敲除小鼠是纯合子存活的，但表现出背部基因的同源转化。SPT16基因敲除小鼠在纯合子中是胚胎致死的。它的杂合子表现出免疫球蛋白亚类水平的偏差。

项目成果

Compensatory change of interacting amino acids in the coevolution of transcriptional coactivator MBF1 and TATA-box-binding protein

• DOI: 10.1093/molbev/msm073
• 发表时间: 2007-07-01
• 期刊: MOLECULAR BIOLOGY AND EVOLUTION
• 影响因子: 10.7
• 作者: Liu, Qing-Xin;Nakashima-Kamimura, Naomi;Gojobori, Takashi
• 通讯作者: Gojobori, Takashi

ショウジョウバエRsf-1の生物学的機能解析

果蝇Rsf-1的生物学功能分析

• 发表时间: 2005
• 作者: Nagata;S.;古橋寛史;大高泉;高橋健太郎;花井一馬
• 通讯作者: 花井一馬

p170 is a labile transcription factor induced by ecdysone in Drosophila melanogaster.

p170 是果蝇中由蜕皮激素诱导的不稳定转录因子。

• 发表时间: 2006
• 作者: Sarhan;M.
• 通讯作者: M.

Histone H3.3 replacement that prevents the heterochromatin spreading.

组蛋白 H3.3 替换可防止异染色质扩散。

• 发表时间: 2005
• 作者: Nakayama;T.
• 通讯作者: T.

Drosophila GAGA factor directs histone H3.3 replacement that prevents the heterochromatin spreading

• DOI: 10.1101/gad.1503407
• 发表时间: 2007-03-01
• 期刊: GENES & DEVELOPMENT
• 影响因子: 10.5
• 作者: Nakayama, Takahiro;Nishioka, Kenichi;Hirose, Susumu
• 通讯作者: Hirose, Susumu