Mechanism of GAGA factor-dependent chromatin remodeling

果蝇GAGA因子指导染色质重塑到其结合位点。为了阐明位点特异性染色质重塑的机制，我们寻找GAGA因子相关蛋白。我们鉴定了两个GAGA因子相关蛋白p93和p130，并对它们进行了分析。结果表明，p93和p130形成异源二聚体，与核小体结合，促进染色质重塑。我们还发现GAGA因子、p93和p130共催化多线染色体上的活性染色质。此外，Trithorax样和p130之间的遗传相互作用暗示GAGA因子-p93-p130复合物在表观遗传基因表达。

Drosophila GAGA factor directs chromatin remodeling to its binding sites. To elucidate the mechanism underlying the site-specific chromatin remodeling, we searched for GAGA factor-associated proteins. We identified two GAGA factor-associated proteins p93 and p130, and analyzed them. The results obtained showed that p93 and p130 form a heterodimer, bind to nucleosome, and facilitate chromatin remodeling. We also found that GAGA factor, p93 and p130 colocalyze to active chromatin on polytene chromosomes. Moreover, genetic interaction between Trithorax-like and p130 implicates GAGA factor-p93-p130 complex in epigenetic gene expression.