Mechanism of GAGA factor-dependent chromatin remodeling

GAGA因子依赖性染色质重塑机制

• 批准号: 11480204
• 负责人: HIROSE Susumu
• 金额: $ 9.54万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11480204/
• 关键词: Drosophila; GAGA factor; chromatic; nucleosome; gene expression; ISWI; Ubx遺伝子; position effect variegation; ISW1

Drosophila GAGA factor directs chromatin remodeling to its binding sites. To elucidate the mechanism underlying the site-specific chromatin remodeling, we searched for GAGA factor-associated proteins. We identified two GAGA factor-associated proteins p93 and p130, and analyzed them. The results obtained showed that p93 and p130 form a heterodimer, bind to nucleosome, and facilitate chromatin remodeling. We also found that GAGA factor, p93 and p130 colocalyze to active chromatin on polytene chromosomes. Moreover, genetic interaction between Trithorax-like and p130 implicates GAGA factor-p93-p130 complex in epigenetic gene expression.

果蝇GAGA因子指导染色质重塑到其结合位点。为了阐明位点特异性染色质重塑的机制，我们寻找GAGA因子相关蛋白。我们鉴定了两个GAGA因子相关蛋白p93和p130，并对它们进行了分析。结果表明，p93和p130形成异源二聚体，与核小体结合，促进染色质重塑。我们还发现GAGA因子、p93和p130共催化多线染色体上的活性染色质。此外，Trithorax样和p130之间的遗传相互作用暗示GAGA因子-p93-p130复合物在表观遗传基因表达。

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