Constitution of the Sites for execution of nuclear functions

执行核职能场所的构成

• 批准号: 07282104
• 负责人: HIROSE Susumu
• 金额: $ 133.18万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-07282104/
• 关键词: Active chromatin; GAGA factor; Chromatin remodeling; Nucleosome; Mini-satellite sequence; Genome imprinting; Matrix associated region; topoisomerase II; クロマチン; ISWIタンパク; インプリンティング; DNAトポイソメラーゼ; 核骨格; Dis2ホスファターゼ; 反復配列; 染色体凝縮; MCMタンパク質; ヘテロ

To elucidate the mechanism of formation of active chromatin, Hirose isolated and analyzed a protein complex that binds to Drosophila GAGA factor and facilitates chromatin remodeling. The GGA repeat in a micro-satellite sequence appears to inhibit nucleosome formation(Kominami). Sasaki found five new enhancers that are involved in imprinting of the mouse Igf2 and H19 genes. Furukawa identified a novel protein that connects lamin B receptor and chromatin, and isolated its cDNA.In the rat cerebellum, topoisomerase IIβ is localized in the nuclear matrix(Tsutui). Kikuchi observed enlargement and degradation of the nucleolus upon aging of a yeast htr1 mutant. Nakaseko isolated a Dis2-associated protein and found it to be Spt5 that regulates elongation by RNA polymerase II.These findings constitute important bases to clarify the mechanisums of formation of active chromatin and genomic imprinting, and to facilitate our understanding of chromatin distribution within the nucleus and function of nuclear matrix.

为了阐明活性染色质形成的机制，Hirose分离并分析了一种与果蝇GAGA因子结合并促进染色质重塑的蛋白质复合物。微卫星序列中的GGA重复似乎抑制核小体形成（Kominami）。Sasaki发现了五种新的增强子，它们参与了小鼠Igf2和H19基因的印记。Furukawa等人发现了一种连接核纤层蛋白B受体和染色质的新蛋白，并分离了其cDNA。菊池观察到酵母htr 1突变体老化后核仁扩大和降解。Nakaseko等分离到一个与Dis2相关的蛋白，并发现它是由RNA聚合酶II调控延伸的Spt5。这些发现为阐明活性染色质和基因组印记的形成机制，为我们理解染色质在细胞核内的分布和核基质的功能提供了重要依据。

项目成果

M. Okada et al.: "Chromatin remodeling mediated by Drosophila GAGA factor and ISWI activates fushi tarazu gene transcription in vitro"Mol. Cell. Biol. 18. 2455-2461 (1998)

M. Okada 等人：“果蝇 GAGA 因子和 ISWI 介导的染色质重塑在体外激活 fushi tarazu 基因转录”Mol。

N.Okishio: "Fission yeast Nda1 and Nda4,MCM homologs required for DNA replication,are constitutive nuclear proteins" Journal of Cell Science. 109. 319-326 (1996)

N.Okishio：“裂殖酵母 Nda1 和 Nda4，DNA 复制所需的 MCM 同源物，是组成型核蛋白”《细胞科学杂志》。

D.R.Kellogg: "Members of the NAP/SET family of proteins interact specifically with B-type cyclins" Journal of Cell Biology. 130. 661-673 (1995)

D.R.Kellogg：“NAP/SET 蛋白家族的成员与 B 型细胞周期蛋白特异性相互作用”《细胞生物学杂志》。

T.Suda: "Formation of a parallel-stranded DNA homoduplex by d(GGA)repeat oligonucleotides" Nucleic Acids Research. 23. 3771-3777 (1995)

T.Suda：“通过 d(GGA) 重复寡核苷酸形成平行链 DNA 同源双链体”核酸研究。

T.Moore: "Multiple imprinted sense and antisense transcipts,differential methylation and tandem repeats in a putative imprinting contorol region upstream of mouse 1gf2" Proc.Natl.Acad.Sci.USA. 94. 12509-12514 (1997)

T.Moore：“小鼠 1gf2 上游推定的印记控制区域中的多个印记有义和反义转录本、差异甲基化和串联重复”Proc.Natl.Acad.Sci.USA。