Constitution of the Sites for execution of nuclear functions

为了阐明活性染色质的形成机制，Hirose分离并分析了一个与果蝇Gaga因子结合并促进染色质重塑的蛋白质复合体。微卫星序列中的GGA重复似乎抑制了核小体的形成(小米)。佐佐木发现了五种新的增强剂，它们与小鼠Igf2和H19基因的印记有关。古川发现了一种连接层蛋白B受体和染色质的新蛋白质，并分离了它的β。在大鼠小脑中，拓扑异构酶II DNA定位于核基质(TUTUI)中。菊口观察到了酵母htr1突变体老化后核仁的增大和降解。Nakaseko分离到一个Dis2相关蛋白，发现它是Spt5，它调控着RNA聚合酶II的伸长。这些发现为阐明活性染色质的形成机制和基因组印迹提供了重要基础，并有助于我们了解染色质在核内的分布和核基质的功能。

To elucidate the mechanism of formation of active chromatin, Hirose isolated and analyzed a protein complex that binds to Drosophila GAGA factor and facilitates chromatin remodeling. The GGA repeat in a micro-satellite sequence appears to inhibit nucleosome formation(Kominami). Sasaki found five new enhancers that are involved in imprinting of the mouse Igf2 and H19 genes. Furukawa identified a novel protein that connects lamin B receptor and chromatin, and isolated its cDNA.In the rat cerebellum, topoisomerase IIβ is localized in the nuclear matrix(Tsutui). Kikuchi observed enlargement and degradation of the nucleolus upon aging of a yeast htr1 mutant. Nakaseko isolated a Dis2-associated protein and found it to be Spt5 that regulates elongation by RNA polymerase II.These findings constitute important bases to clarify the mechanisums of formation of active chromatin and genomic imprinting, and to facilitate our understanding of chromatin distribution within the nucleus and function of nuclear matrix.