Development of Bone-bonding Bioactive Titanium Using Nanotechnology and its' Application for Orthopedic Implant.

1.研究了表面带有纳米孔锐钛矿层的阳极氧化钛的纳米孔结构、体外生物活性和体内骨结合能力。在1M-H_2SO_4溶液中，经150V和200V阳极氧化处理的AO钛板分别植入兔胫骨近端干骺端4、8、16、24周，进行光镜、扫描电子显微镜和剥离实验观察。2.制备了不同类型的含纳米二氧化钛的生物活性聚甲基丙烯酸甲酯(PMMA)基骨水泥，并对其力学性能和骨传导性进行了评价。以市售PMMA水泥(PMMAc)作为对照。将骨水泥植入大鼠的胫骨，并允许其原位固化。在6周和12周后取出胫骨进行骨传导性…评估扫描电子显微镜(SEM)、接触式显微放射照相(CMR)和Giemsa表面染色。含有纳米金红石或锐钛矿的PMMA基骨水泥表现出比市售PMMA骨水泥更好的骨传导性能。3.研究了纳米生物活性多孔表面与微孔结构的关系，基于微焦点X射线计算机层析成像系统，对不同孔径和孔隙率(直径6 mm，长15 mm)的生物活性多孔钛种植体进行了三维分析。在体内进行组织形态计量学分析，使用相同的植入物分别植入6周和12周的雄性兔股骨髁部。这项配对研究分析揭示了广泛的互连(直径大于52μm)对于骨生长到具有纳米二氧化钛表面的多孔结构中的重要性。较少

1.We investigated nanoporous structure, in vitro bioactivity, and in vivo bone-bonding ability of anodically oxidized titanium (AO Ti) with a nanoporous anatase crystal layer on its surface. AO Ti plates, anodically oxidized at 150V and 200V in 1 M-H_2SO_4, were implanted into the proximal metaphyses of mature rabbit tibiae for 4, 8, 16, and 24 weeks and investigated by light microscopy, scanning electron microscopy and detaching test. High bone-bonding ability, comparable to our previous study data of the bioactive titanium, was observed during post implantation times.2.Various types of bioactive polymethylmethacrylate (PMMA)-based bone cement containing nano-sized titania (TiO2) particles were prepared, and their mechanical properties and osteoconductivity are evaluated. Commercially available PMMA cement (PMMAc) was used as a control. The cements were inserted into rat tibiae and allowed to solidify in situ. After 6 and 12 weeks, tibiae were removed for evaluation of osteoconductivi … More ty using scanning electron microscopy (SEM), contact microradiography (CMR), and Giemsa surface staining. The PMMA-baced cement with nanosized rutile or anatase showed superior osteoconduction compared to the commercially available PMMA cement. These results show that PMMA-based bone cement containing nanosized titania particles is a promising material for use as a bone substitute.3.We investigated the relationship between nanosized bioactive porous surface and micronsized macropore structure, various types of bioactive porous titanium implants with different pore sizes and porosities (6mm in diameter and 15mm long) were analyzed using specific algorithms for 3D analysis of interconnectivity based on a micro focus X-ray computed tomography system. In vivo histomorphometric analysis was performed using the very same implants implanted into the femoral condyles of male rabbits for 6 and 12 weeks. This matching study analysis revealed the importance of wide interconnections (diameter more than 52 μm) for bone ingrowth into porous structure with nanosized titania surface. Less