A study of the healing mechanisms of destructive periapical lesions and regenerative medicine for periapical bone defect

破坏性根尖周病变愈合机制及根尖周骨缺损再生医学研究

• 批准号: 16209056
• 负责人: ANAN Hisashi
• 金额: $ 30.12万
• 依托单位: Fukuoka Dental College (2006)Kyushu University (2004-2005)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16209056/
• 关键词: periapical lesion; regenerative medicine; scaffold; periodontal ligament cell; enamel matrix protein; FGF-2; PRP; BMP; 骨芽細胞; 再生医療; CBF; PGE2

It has been reported that there are three key factors, such as cells, scaffolds and signaling molecules (growth factors), in the regenerative medicine. However, regenerative mechanisms of large-size defects in periapical lesions are not yet well understood. This study presented herein was therefore undertaken to define the progression and healing mechanisms in periapical lesions, and the effects of regenerative materials was investigated. It was showed that transplanted proliferating tissue produced by GTR membrane promoted the formation of new periodontal ligament(PDL) around the tooth. We have established three immortal human PDL fibroblast line by transfecting SV40T-Ag and hTERT into primary PDL fibroblasts.These immortalized cells was useful models for elucidating the biological features and regenerative mechanisms of human PDL. Mineral Trioxide Aggregate could up-regulated osteopontin and osteocalcin mRNA in human PDL to induce their differentiation. FGF-2 enhanced hyaluronan prod … More uction by both human dental pulp cell and human PDL cell and hyaluronan synthase (HAS)1 and HAS2mRNA expression in both cells. Immune and nervous systems play key roles in periapical pathosis, and functional interactions between antigen-presenting cells and nerve fibers may play some roles in the development of self-defense reactions in periapical lesions. In addition, expression of RANKL is correlated with periapical lesion expansion, and followed by the expressions of RANK and OPG. Platelet-rich plasma (PRP) and washed platelets were potent inhibitors of RANKL-induced osteoclast differentiation in RAW264.7 cells. After application of Emdogain containing enamel matrix protein to the root surface, the formation of new cementum and more remarkable recovery of the bone tissue were observed. Although the expression of cytokines, such as IL-1β, RANKL, and RANK were hardly seen, BMP-2and BMP-4 expressing macrophages were increased. It was suggested that wound healing macrophages may express BMP and play an important role in the regeneration of periodontal tissue at the apex in EMD application. Less

据报道，在再生医学中有三个关键因素，即细胞、支架和信号分子（生长因子）。然而，根尖周病变中大面积缺损的再生机制尚未完全了解。因此，本研究旨在确定根尖周病变的进展和愈合机制，并研究再生材料的作用。实验结果表明，GTR膜产生的增殖组织移植到牙齿周围，促进了新牙周膜（PDL）的形成。本研究通过将SV 40 T-Ag和hTERT转染原代人牙周膜成纤维细胞，建立了三株永生化的人牙周膜成纤维细胞系，为进一步研究人牙周膜的生物学特性和再生机制提供了有用的模型。三氧化物矿物质聚集体可上调人牙周膜骨桥蛋白和骨钙素mRNA的表达，诱导其分化。FGF-2增强透明质酸生成 ...更多信息 人牙髓细胞和人牙周膜细胞中透明质酸合成酶（HAS）1和HAS 2 mRNA的表达。免疫和神经系统在根尖周病变中起关键作用，抗原呈递细胞和神经纤维之间的功能相互作用可能在根尖周病变的自我防御反应中起一定作用。RANKL的表达与根尖周病变的扩大相关，RANK和OPG的表达次之。富血小板血浆（PRP）和洗涤血小板是RAW264.7细胞中RANKL诱导破骨细胞分化的强效抑制剂。将含有釉基质蛋白的Emdogain应用于牙根表面后，观察到新牙骨质的形成和更显著的骨组织恢复。虽然细胞因子如IL-1β、RANKL和RANK的表达几乎不见，但表达BMP-2和BMP-4的巨噬细胞增加。提示创面愈合巨噬细胞可能表达BMP，在EMD修复根尖周组织再生中起重要作用。少

项目成果

Microflora profiling of root canal utilizing real-time PCR and cloning sequence analysis based 16S rRNA genes-Deference between before and after root canal treatments-

利用实时 PCR 和基于 16S rRNA 基因的克隆序列分析进行根管微生物区系分析-根管治疗前后的差异-

• 发表时间: 2007
• 期刊: The Japanese Journal of Conservative Dentistry 50(in press)
• 作者: Yasuhiro Ito;Takichi Sato;Gen Mayanagi;Keiko Yamaki;Nobuhiro Takahashi;Hidetoshi Shimauchi
• 通讯作者: Hidetoshi Shimauchi

歯根穿孔に由来する歯内-歯周疾患に類似した病変の1症例

牙根穿孔引起的类似牙髓-牙周病的病变一例

• 发表时间: 2005
• 期刊: 日本歯科保存学雑誌 47
• 作者: 生水真紀夫;山澤功二;三橋暁;確井宏和;木原真紀;阿南 壽
• 通讯作者: 阿南 壽

A case of lesion mimicking endodontic-periodontal disease caused by crestal root perforation

牙槽嵴根穿孔引起的类似牙髓-牙周病的病变一例

• 发表时间: 2005
• 期刊: The Japanese Journal of Conservative Dentistry 48(1)
• 作者: Anan Hisashi;Nakanishi Miho;Matsumoto Akiko;Yoneda Masahiro;Kimura Ryusei;Kabashima Hiroaki;maeda Katsumasa
• 通讯作者: maeda Katsumasa

Pathological aspects of pulpal and periapical inflammation in Essential Endodontology

基础牙髓病学中牙髓和根尖周炎症的病理学方面

• 发表时间: 2007
• 作者: 林田賢史;今中雄一;桑原一彰;他.;Mizuki N;生水真紀夫;越智光夫;Kawashima N
• 通讯作者: Kawashima N

Kinetic study of immunohistochemical colocalization of antigen presenting cells and nerve fibers in rat periapical lesions.

大鼠根尖周病变中抗原呈递细胞和神经纤维的免疫组织化学共定位的动力学研究。

• 发表时间: 2007
• 期刊: Journal of Endodontics 33(2)
• 作者: Yang G;Kawashima N;Kaneko T;Suzuki N;Okiji T;Suda H
• 通讯作者: Suda H