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Expression of IL-1 and adhesion molecules during osteoclast development in rat periapical lesions

大鼠根尖周病变破骨细胞发育过程中IL-1和粘附分子的表达

基本信息

批准号：
08672198
负责人：
ANAN Hisashi
金额：
$ 1.28万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672198/
关键词：
Adhesion molecule ICAM-1 LFA-1 Osteoclast Macrophage IL-1 beta IL-1B 骨芽細胞マイクロファージインテグリンβ_3

项目摘要

In order to investigate the pathobiological mechanisms of bone destruction during apical periodontitis, we examined the relationship between the expression of interleukin-1 (IL-1) and that of adhesion molecules, such as intercellular adhesion molecule-1 (ICAM-1) and leukocyte function- associated antigen-1 (LFA-1), in rat periapical lesions using immunohistochemical methods. During the acute phase, IL-1-expressing cells were observed adjacent to many osteoclasts which contained large cytoplasms, and consequently the periapical lesions tended to expand. The number of IL-1 beta -expressing cells was about twice that of IL-1 alpha -expressing cells, and the expression of adhesion molecules ICAM-1 and LFA-1 were increased in the vicinity of the bone resorption surfaces during the same period. On the other hand, there were significantly less IL-1 beta -expressing cells during the chronic phase and consequently a significant expansion of periapical lesions was not seen. Furthermore, IL-1 beta -expressing cells were located far from the bone surfaces, and the expression of ICAM-1 and LFA-1 was decreased near the bone surfaces. Both IL-1 alpha and IL-1 beta were mainly expressed by macrophages. These results suggest that macrophages expressing IL-1 beta may play an important role in the activation of osteoclastic bone resorption and this may be due to the increase expression of ICAM-1 and LFA-1 in the vicinity of the bone resorption surfaces within experimental periapical lesions.

为探讨根尖周炎时骨质破坏的病理生物学机制，采用免疫组织化学方法检测了白细胞介素1(IL-1)在大鼠根尖周病变中的表达与细胞间黏附分子-1(ICAM-1)、白细胞功能相关抗原-1(LFA-1)等黏附分子表达的关系。急性期，在大量破骨细胞旁可见表达IL-1的细胞，这些破骨细胞含有较大的细胞质，因此根尖周病变有扩大的趋势。在同一时期，IL-1β表达细胞的数量约为IL-1α表达细胞的两倍，而黏附分子ICAM-1和LFA-1在骨吸收表面附近的表达增加。另一方面，在慢性期，表达IL-1β的细胞显著减少，因此没有看到根尖周围病变的显著扩张。此外，IL-1β表达的细胞远离骨表面，ICAM-1和LFA-1在靠近骨表面的表达减少。IL-1α和IL-1β主要由巨噬细胞表达。这些结果提示，表达IL-1β的巨噬细胞可能在破骨细胞性骨吸收的激活过程中起重要作用，这可能是由于实验性根尖周病灶骨吸收表面ICAM-1和LFA-1表达增加所致。