TIRF Super-resolution microscope

TIRF超分辨率显微镜

• 批准号: 450216812
• 金额: --
• 依托单位: Albert-Ludwigs-Universität Freiburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/450216812?language=en
• 关键词: TIRF; Super; resolution; microscope

At the Institute of Biochemistry and Molecular Biology of the Faculty of Medicine (University of Freiburg), nine research groups study molecular mechanisms underlying cell function, with a focus on membrane biogenesis, movement and turnover, mitochondrial organization, autophagy with formation and dynamics of autophagosomes, ribosome assembly and dynamics. Since the biochemical projects now have developed a high demand for advanced live cell imaging, a microscope with a flexible and advanced operation is highly needed. In all our projects, small structures or vesicles are involved, which are impossible to resolve by wide-field microscopy. To understand the dynamics, assemblies and substructure of the structures, we need advanced microscopy techniques including super-resolution microscopy, which allows to analyze protein complexes and their dynamics in living cells as well as protein assemblies of in vitro reconstitutions. Especially for the analysis of substructures also in mammalian cells, wide field microscopy is limiting, as it does not provide enough contrast to see small punctate objects such as autophagosomes. We apply for an integrated super-resolution microscopy system, which allows a similar workflow for all of the setups and therefore an efficient training of the graduate students and postdocs as well as an easy usage and maintenance. The microscope will fulfill a major demand of the nine research groups of the Institute of Biochemistry and Molecular Biology (including two ERC grants, a DFG Koselleck project, a Heisenberg project, an Emmy- Noether project, seven projects of the new SFB, and numerous projects in the cluster of excellence CIBSS and in graduate schools).

在医学系生物化学和分子生物学研究所（弗赖堡大学），九个研究小组研究细胞功能的分子机制，重点是膜生物发生，运动和周转，线粒体组织，自噬与自噬体的形成和动力学，核糖体组装和动力学。由于生物化学项目现在对先进的活细胞成像有很高的要求，因此非常需要一种具有灵活和先进操作的显微镜。在我们所有的项目中，都涉及到小结构或囊泡，这是宽视场显微镜无法解决的。为了了解结构的动力学，组装和亚结构，我们需要先进的显微镜技术，包括超分辨率显微镜，它允许分析蛋白质复合物及其在活细胞中的动力学以及体外重组的蛋白质组装。特别是对于哺乳动物细胞中的亚结构的分析，宽视野显微镜是有限的，因为它不能提供足够的对比度来看到小的点状物体，如自噬体。我们申请了一个集成的超分辨率显微镜系统，它允许所有设置的类似工作流程，因此研究生和博士后的有效培训以及易于使用和维护。该显微镜将满足生物化学和分子生物学研究所的九个研究小组的主要需求（包括两个ERC赠款，DFG Koselleck项目，海森堡项目，Emmy- Noether项目，新SFB的七个项目，以及卓越CIBSS和研究生院的众多项目）。