The analysis of gene expressions related to yield in rice bared on genome information

基于基因组信息的水稻产量相关基因表达分析

• 批准号: 14206004
• 负责人: OHSUGI Ryu
• 金额: $ 32.7万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14206004/
• 关键词: Rice; Yield; Gene expression; Macroarray; Superior caryopses; Inferior caryopses; Rice genome; Introgression lines; マクロアレイ; QTL解析; 登熟; 炭素代謝; 品種間差; イネ収量関連形質; 強勢頴果と弱勢頴果; 網羅的遺伝子解析

The object of this research is to elucidate some factors responsible for high yield in rice and its varietal differences analyzing comprehensively gene expressions related to yield in rice and quantitative trait loci (QTL).(1)There were large differences in gene expression profile of some enzymes related to carbohydrate metabolism between superior and inferior caryopses of rice and there differences were closely associated with developmental stage of endosperm. (2)Some genes of storage proteins were especially expressed in superior caryopses and, on the other hand, some genes encoding glycine-rich proteins were highly expressed in inferior caryopses based on the analysis using cDNA libraries which were developed with caryopses obtained at temporarily after flowering. (3)Macmarray analysis of 296 genes involved in carbohydrate metabolism showed that there were two types of genes; the one was expressed highly during the early stage of caryopsis development, the other was expressed highly during the middle stage. (4) Several introgression lines which showed significant differences in the traits related to culm length, panicle number chlorophyll content and specific leaf weight from Koshihikari were found in 39 introgression lines with a chromosome segment of Kasalath, in the genetic background of Koshihikari.

本研究的目的是通过对水稻产量相关基因表达及数量性状位点（QTL）的综合分析，阐明水稻高产的一些因素及其品种差异。（1）水稻优劣颖果中一些与碳水化合物代谢相关的酶基因表达谱存在较大差异，且这些差异与胚乳发育阶段密切相关。 (2)根据利用开花后暂时获得的颖果开发的cDNA文库进行分析，一些贮藏蛋白基因在上等颖果中特别表达，另一方面，一些编码富含甘氨酸的蛋白质的基因在下等颖果中高表达。 (3)Macmarray分析了296个与碳水化合物代谢相关的基因，结果显示有两类基因；一种在颖果发育早期高表达，另一种在中期高表达。 (4)在越光遗传背景下,在具有Kasalath染色体片段的39个渗入系中发现了几个与越光的秆长、穗数、叶绿素含量和比叶重相关性状表现出显着差异的渗入系。

项目成果

大杉 立: "ショ糖の転流に関する分子機構の解明と作物への応用"化学と生物. (印刷中). (2003)

Tate Osugi：“蔗糖易位的分子机制及其在作物中的应用的阐明”化学与生物学（出版中）。

Temporal and Spatial Variations of Carbohydrate Content in Rice Leaf Sheath and Their Varietal Differences

• DOI: 10.1626/pps.8.546
• 发表时间: 2005-01
• 期刊: Plant Production Science
• 影响因子: 2.5
• 作者: Hai He;M. Koike;T. Ishimaru;R. Ohsugi;T. Yamagishi

Expression patterns of genes encoding carbohydrate-metabolizing enzymes and their relation to grain filling in rice (Oryza sativa L.).

编码碳水化合物代谢酶的基因的表达模式及其与水稻籽粒灌浆的关系（Oryza sativa L.）。

• 发表时间: 2005
• 期刊: Plant Cell and Physiology
• 作者: Ishimaru;T. et al.
• 通讯作者: T. et al.

Morphological development of rice caryopses located at the different positions in a panicle from early to middle stage of grain fillin

水稻灌浆早中期穗部不同位置颖果的形态发育

• 发表时间: 2003
• 期刊: Functional Plant Biol. 30
• 作者: Ishimaru;T.;T.Matsuda;R.Ohsugi;T.Yamagishi
• 通讯作者: T.Yamagishi

Expression patterns of genes encoding carbohydrate-metabolizing enzymes and their relation to grain filling in rice (Oryza sativa L.) : comparison of caryopses located at different positions in a panicle.

编码碳水化合物代谢酶的基因的表达模式及其与水稻籽粒灌浆的关系：位于圆锥花序中不同位置的颖果的比较。

• 发表时间: 2005
• 期刊: Plant Cell Physiol. 46
• 作者: Ishimaru T.;Hirose;T.Matsuda;A.Goto;K.Takahashi;H.Sasaki;T.Terao;R.Ishii;R.Ohsugi;T.Yamagishi
• 通讯作者: T.Yamagishi