Wide field microscope for live cell microscopy

所申请的设备将广泛用于生物、医学和生物材料的研究。有了这项技术，将有可能首次解决问题，特别是在生命系统（细胞、类器官、组织切片和生物材料）中，这些问题以前只能通过结合不同的技术来实现。现在可以在长时间内以高穿透深度和高分辨率在活样品中拍摄图像，非常快速（空间和时间）并且对样品温和。这将应用于不同物种（小鼠，人类）和来源（肠道，神经元，卵巢）的类器官，以研究空间和时间维度上的不同问题。神经类器官中的钙稳态和谷氨酸释放，肠道类器官中的炎症过程或神经类器官中转录因子如Sox-11或TCF4的分布和调控。异种移植小鼠肿瘤组织切片的研究。植物中蛋白质分布的研究，即根、茎和叶。另一个应用计划是在生物材料研究领域的卵泡三维基质。在不同的类器官模型上建立屏障完整性分析是另一个重点。

The device applied for is to be used in a wide range of biological, medical and biomaterials research. With this technology, it will be possible for the first time to address questions, especially in living systems (cells, organoids, tissue sections and biomaterials), which were previously only possible by combining different technologies. It is now possible to take images in living samples with high penetration depth and high resolution, very fast (spatially & temporally) and gentle on the sample, over long periods of time. This will be applied to organoids of different species (murine, human) and origin (intestinal, neuronal, ovarian) to investigate different questions in spatial and temporal dimensions. Calcium homeostasis and glutamate release in neuronal organoids, inflammatory processes in intestinal organoids or the distribution and regulation of transcription factors such as Sox-11 or TCF4 in neuronal organoids. Studies in tumor tissue sections from xeno-graft mice. The investigation of protein distributions in planta, i.e. roots, stems and leaves. Another application is planned in the field of biomaterial research on follicles in three-dimensional matrices. The establishment of barrier integrity assays on different organoid models is another focus.