Wide field microscope for live cell microscopy

用于活细胞显微镜检查的宽视野显微镜

• 批准号: 450993414
• 金额: --
• 依托单位: Friedrich-Alexander-Universität Erlangen-Nürnberg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/450993414?language=en
• 关键词: Wide; field; microscope; live; cell

The device applied for is to be used in a wide range of biological, medical and biomaterials research. With this technology, it will be possible for the first time to address questions, especially in living systems (cells, organoids, tissue sections and biomaterials), which were previously only possible by combining different technologies. It is now possible to take images in living samples with high penetration depth and high resolution, very fast (spatially & temporally) and gentle on the sample, over long periods of time. This will be applied to organoids of different species (murine, human) and origin (intestinal, neuronal, ovarian) to investigate different questions in spatial and temporal dimensions. Calcium homeostasis and glutamate release in neuronal organoids, inflammatory processes in intestinal organoids or the distribution and regulation of transcription factors such as Sox-11 or TCF4 in neuronal organoids. Studies in tumor tissue sections from xeno-graft mice. The investigation of protein distributions in planta, i.e. roots, stems and leaves. Another application is planned in the field of biomaterial research on follicles in three-dimensional matrices. The establishment of barrier integrity assays on different organoid models is another focus.

申报器械将用于广泛的生物、医学和生物材料研究。有了这项技术，将首次有可能解决问题，特别是在生命系统（细胞，类器官，组织切片和生物材料）中，这些问题以前只能通过结合不同的技术来解决。现在可以在活体样品中拍摄具有高穿透深度和高分辨率的图像，非常快速（空间和时间），并且在长时间内对样品进行温和处理。这将应用于不同物种（小鼠，人类）和起源（肠道，神经元，卵巢）的类器官，以研究空间和时间维度的不同问题。神经元类器官中的钙稳态和谷氨酸释放，肠类器官中的炎症过程或神经元类器官中转录因子如Sox-11或TCF 4的分布和调节。异种移植小鼠肿瘤组织切片研究。植物根、茎、叶中蛋白质分布的研究。另一项应用计划是在三维基质中卵泡的生物材料研究领域。在不同类器官模型上建立屏障完整性测定是另一个焦点。