Stabilization of the life cycle of temperate fruit trees under irregular climates - elucidation of the molecular mechanism on water movement and redox regulation in the dormant buds

不规则气候下温带果树生命周期的稳定——阐明休眠芽水分运动和氧化还原调节的分子机制

• 批准号: 18208003
• 负责人: GEMMA Hiroshi
• 金额: $ 30.04万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-18208003/
• 关键词: 環境; 気候変動; 温暖化; 休眠生理; 果樹; MRI; レドックス; 水チャネル; ネクロシス

The transition from bud endodormancy state was characterized by a significant increasing on glutathione content in dormant bud tissues. The GSH accumulation in the buds was coincided with major increases in transcript patterns of genes GSH1 and GSH2 and redox regulating enzyme genes such as the alternative oxidase (AOX). According to the findings by MRI observation, a gradual increase in water content to that required for peach dormant bud activity at the end of endodomancy was resulted from inter- and intracell communication through membrane transport properties of aquaporins. And also, the mixed buds of Japanese pear subjected to low chilling accumulation showed low water mobility and water content especially in scales. In contrast, increased water mobility and relative content were observed in specific portions, which was more accentuated in the mixed buds of plants grown for several seasons under mild winter conditions, resulting in the necrosis of floral primordia, and the development of new inflorescences which corresponds an irregular floral form.

从芽内休眠状态转变为休眠芽组织中谷胱甘肽含量显著增加。芽中GSH的积累与GSH 1和GSH 2基因以及氧化还原调节酶基因（如交替氧化酶（AOX））的转录模式的主要增加相一致。根据MRI观察的结果，水含量逐渐增加到所需的桃休眠芽活动的结束时，内优势是由于通过水通道蛋白的膜运输特性的细胞间和细胞内的通信。低冷量处理下，日本梨混合芽的水分流动性和含水量均较低，尤其是鳞片。与此相反，增加水的流动性和相对含量，观察到在特定的部分，这是更强调在温和的冬季条件下生长了几个季节的植物的混合芽，导致花原基坏死，和新的花序，这对应于一个不规则的花的形式的发展。

项目成果

Effect of oscillating temperature on the expression of two aquaporin genes (Pp-δTIP1, Pp-PIP2) involved in regulating intercellular water status in flower buds of peach

振荡温度对桃花芽细胞间水分状态调节的两个水通道蛋白基因（Pp-δTIP1、Pp-PIP2）表达的影响

• 发表时间: 2008
• 期刊: J.Hort.Sci.Biotech. 83
• 作者: Yooyongwech;S.;A. Horigane;M. Yoshida;Y. Sekozawa;S. Sugaya;H. Gemma
• 通讯作者: H. Gemma

Floral primordial necrosis incidence in mixed buds of Japanese pear (Pyrus pyrifolia (Burm.) Nakai var. culta) 'Housui' grown under mild winter conditions and the possible relation with water dynamics

温和冬季条件下生长的日本梨 (Pyrus perifolia (Burm.) Nakai var. culta) Housui 混合芽花原基坏死发生率及其与水动态的可能关系

• 发表时间: 2010
• 期刊: J.Japan.Soc.Hort.Sci. (Inpress)
• 作者: Yamamoto;R. R.;A. K. Horigane;M. Yoshida;Y. Sekozawa;S. Sugaya;H. Gemma
• 通讯作者: H. Gemma

Floral primordial necrosis incidence in mixed buds of Japanese pear(Pyruspyrifolia(Burm.)Nakai var.culta)'Housui'grown under mild winter conditions and the possible relation with water dynamics

温和冬季条件下生长的日本梨(Pyruspyrifolia(Burm.)Nakai var.culta)Housui混合芽花原基坏死发生率及其与水分动态的可能关系

• 发表时间: 2010
• 期刊: J.Japan.Soc.Hort.Sci (In press)
• 作者: Yamamoto;R.R.;Horigane;A.K.;Yoshida;M.;Sekozawa;Y.;Sugaya;S.;Gemma;H.
• 通讯作者: H.

Influence of Chilling Accumulation Time on Flower Bud Abortion Occurrence in Japanese Pear Grown under Mild Winter Conditions

• DOI: 10.17660/actahortic.2010.872.6
• 发表时间: 2010-08
• 作者: R. R. Yamamoto-R.;Y. Sekozawa;S. Sugaya;H. Gemma