Integrated construction of genotypic and phenotypic data of microorganisms relating to the cause of deterioration of the Takamatsuzuka Tumulus mural paintings

本研究旨在探讨高松冢壁画生物劣化过程中的微生物多样性。为了阐明生物降解的原因，我们仔细确定使用表型和基因型性状的综合分析。还使用相同的方法在属级水平鉴定了主要细菌分离株。因此，与壁画生物劣化有关的主要真菌类群可归属于茄病镰刀菌种复合体，一些木霉属，顶孢霉属（胶鞭菌组）和青霉属。除霉菌外，一些念珠菌属和毕赤酵母属以及细菌物种也参与了石膏壁上生物膜的形成。本研究所选菌株及其DNA序列测定结果分别保存在公共培养物保藏中心和公共基因库。

This study aimed to investigate the microbial diversity which was involved in the biodeterioration of murals in the Takamatsuzuka Tumulus. To elucidate the cause of biodeterioration, we carefully identified using an integrated analysis of phenotypic and genotypic characters. The major bacterial isolates were also identified at the generic level using the same methodology. As a result, the major fungal taxa relating to biodeterioration of murals were assignable to Fusarium solani species complex, some Trichoderma spp., Acremonium (sect. Gliomastix) masseei and Penicillium paneum. Some Candida and Pichia and bacterial species in addition to molds species were involved in the formation of biofilms on plaster walls. The selected isolates and their DNA sequences determined in this study were deposited in the public culture collection and public gene bank, respectively.