Integrated construction of genotypic and phenotypic data of microorganisms relating to the cause of deterioration of the Takamatsuzuka Tumulus mural paintings

本研究旨在探讨高松冢墓室壁画生物降解过程中微生物的多样性。为了阐明生物变质的原因，我们使用表型和基因型特征的综合分析仔细确定。使用相同的方法也在一般水平上鉴定了主要的细菌分离株。结果表明，与壁画生物降解有关的主要真菌类群为番茄镰刀菌（Fusarium solani）、部分木霉（Trichoderma）、胶质瘤青霉（Acremonium Gliomastix）和paneum。石膏壁上生物膜的形成除霉菌外，还包括念珠菌、毕赤酵母和其他细菌。选择的分离株及其DNA序列分别存放于公共培养库和公共基因库。

This study aimed to investigate the microbial diversity which was involved in the biodeterioration of murals in the Takamatsuzuka Tumulus. To elucidate the cause of biodeterioration, we carefully identified using an integrated analysis of phenotypic and genotypic characters. The major bacterial isolates were also identified at the generic level using the same methodology. As a result, the major fungal taxa relating to biodeterioration of murals were assignable to Fusarium solani species complex, some Trichoderma spp., Acremonium (sect. Gliomastix) masseei and Penicillium paneum. Some Candida and Pichia and bacterial species in addition to molds species were involved in the formation of biofilms on plaster walls. The selected isolates and their DNA sequences determined in this study were deposited in the public culture collection and public gene bank, respectively.