Elucidation of the functional mechanism of the novel 2-component K^+/H^+ antiporters found in Escherichia coli and Bacillus subtilis.
Elucidation of the functional mechanism of the novel 2-component K^+/H^+ antiporters found in Escherichia coli and Bacillus subtilis.
批准号:
21870035
负责人:
FUJISAWA Makoto
金额:
$1.76万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Research Activity Start-up
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2010
中文摘要
为阐明大肠杆菌和枯草芽孢杆菌中由膜蛋白和外周蛋白组成的新型双组分K^+/H^+反向转运蛋白的功能机制,建立了其相互作用的检测体系。将肽标签引入到蛋白质中,并从B研究蛋白质之间的相互作用。pull-down和blue-native PAGE均成功检测到枯草芽孢杆菌。
英文摘要
To elucidate the functional mechanism of the novel 2-component K^+/H^+ antiporters composed from a membrane protein and a peripheral protein found in Escherichia coli and Bacillus subtilis, the detection systems of its interaction was established. Peptide tags were introduced into the proteins and the interaction between the proteins from B. subtilis was successfully detected by both pull-down assay and blue-native PAGE.
期刊论文(4)
专著(0)
科研奖励(0)
会议论文
Detection of the interaction between membrane protein YhaU and hydrophilic protein YhaT of the 2-component K^+/H^+ antiporter from Bacillus subtilis.
检测枯草芽孢杆菌 2 组分 K^ /H^ 逆向转运蛋白的膜蛋白 YhaU 和亲水蛋白 YhaT 之间的相互作用。
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[Shinichiro Nakada, et al., 今村壮輔, 藤澤誠]
通讯作者:
藤澤誠
CPA2ファミリーに属する2成分K^+/H^+アンチポーターのサブユニット間の相互作用の検出
检测属于 CPA2 家族的双组分 K^+/H^+ 反向转运蛋白亚基之间的相互作用
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[Sowa, Y., 藤澤誠]
通讯作者:
藤澤誠
CPA2ファミリーに属する2成分K+/H+アンチポーターのサブユニット間の相互作用の検出
检测属于 CPA2 家族的双组分 K+/H+ 反向转运蛋白亚基之间的相互作用
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[Shinichiro Nakada, et al., 今村壮輔, 藤澤誠, 佐藤薫, 中田慎一郎, 藤澤誠]
通讯作者:
藤澤誠
Study of High-quality Surface Generation Technology for Numerical Simulation
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批准号:25730069
-
项目类别:Grant-in-Aid for Young Scientists (B)
-
资助金额:$2.0万
-
财政年份:2013
-
负责人:FUJISAWA Makoto
-
依托单位:
The development for the model of human resource cultivation for improving the quality of discharge support which is based on user's needs
-
批准号:24593449
-
项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.41万
-
财政年份:2012
-
负责人:FUJISAWA Makoto
-
依托单位:
Purification and functional analysis of YhaTU system, a two component K+/H+ antiporter from Bacillus subtilis
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批准号:23770156
-
项目类别:Grant-in-Aid for Young Scientists (B)
-
资助金额:$2.91万
-
财政年份:2011
-
负责人:FUJISAWA Makoto
-
依托单位:
Study of Interaction Technology with Augmented Reality Interface and Physics Based Simulation
-
批准号:21700110
-
项目类别:Grant-in-Aid for Young Scientists (B)
-
资助金额:$2.66万
-
财政年份:2009
-
负责人:FUJISAWA Makoto
-
依托单位:
海外基金