Structural and biochemical analyses of interactions among TSWV structural proteins
Structural and biochemical analyses of interactions among TSWV structural proteins
批准号:
22880001
负责人:
KOMODA Keisuke
金额:
$2.01万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Research Activity Start-up
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2011
中文摘要
番茄斑萎病毒(TSWV)的核衣壳蛋白(NP)是病毒RNA复制的关键蛋白。在本研究中,我们获得了衍射至2。7分辨率不对称单元包含三个NP分子,形成三聚体环状结构。此外,TSWV NP三聚体环的内部显示正电荷丰富的表面,表明这些区域预期是基因组RNA结合位点。
英文摘要
The nucleocapsid proteins(NP) of Tomato Spotted Wilt Virus(TSWV) are key proteins in the replication of viral RNA. In this study, we obtained crystals of recombinant TSWV NPs diffracting to 2. 7Åresolution. The asymmetric unit contains three NP molecules forming trimeric ring-like structure. Moreover, the inside of TSWV NP trimeric ring shows the positive charge rich surface, suggesting that these regions are expected to be genomic RNA binding site.
期刊论文(2)
专著(0)
科研奖励(0)
会议论文
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[成田聖実, 石橋洋平, 石橋洋平, 成田聖実]
通讯作者:
成田聖実
海外基金