Endoplasmic reticulum stress is involved in H. Pylori associated gastritis

内质网应激与幽门螺杆菌相关性胃炎有关

• 批准号: 22890138
• 负责人: AKAZAWA Yuuko
• 金额: $ 1.81万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22890138/
• 关键词: VacA; ヘリコバクターピロリ; BH3オンリー蛋白; アポトーシス; ER stress; ヘリコバクター・ピロリ

Apoptosis by H. pylori vactuolating cytotoxin(VacA) plays a role in H. Pylori induced apoptosis in gastric epitherial cells. However, precise mechanism of VacA apoptosis is not understood. Our aim was to examine involvement of ER stress and pro-apoptotic BH3 only proteins in human H. Pylori infection and in VacA treated cells. GADD34 mRNA and GRP 78 mRNA were significantly increased in H. Pylori infected gastric mucosa. Immunohistochemistry showed CRP78 staining in gastric epitherial cells. Apoptosis inducing ER stress marker CHOP was significantly elevated in AZ521 treated with VacA, and was contributing to apoptosis. The present study is, to our knowledge, among the first to implicate the association of ER stress with human H. Pylori infection and its apoptosis inducing toxin, VacA.

幽门螺杆菌空泡毒素(VacA)诱导的胃上皮细胞凋亡在Hp诱导的胃上皮细胞凋亡中起重要作用。然而，VacA细胞凋亡的确切机制尚不清楚。我们的目的是检测内质网应激和促凋亡的BH3蛋白在人幽门螺杆菌感染和VacA处理的细胞中的参与。幽门螺杆菌感染的胃粘膜组织中Gadd34和GRP78mRNA表达显著增加。免疫组织化学显示CRP78在胃上皮细胞中表达。AZ521经VacA处理后，细胞凋亡诱导ER应激标记物CHOP显著升高，并参与细胞凋亡。据我们所知，本研究是最早将内质网应激与人幽门螺杆菌感染及其凋亡诱导毒素VacA联系起来的研究之一。

项目成果

Glycogen synthase kinase-3 (GSK-3) inhibition attenuates hepatocyte lipoapoptosis.

• DOI: 10.1016/j.jhep.2010.09.039
• 发表时间: 2011-04
• 期刊: Journal of hepatology
• 影响因子: 25.7
• 作者: Ibrahim SH;Akazawa Y;Cazanave SC;Bronk SF;Elmi NA;Werneburg NW;Billadeau DD;Gores GJ
• 通讯作者: Gores GJ

The Mechanisms by Which Unsaturated free fatty Acid Attenuates Saturated Free Fatty Acid Mediated Apoptosis

不饱和游离脂肪酸减弱饱和游离脂肪酸介导的细胞凋亡的机制

• 发表时间: 2010
• 作者: Yuko Akazawa;Hajime Isomoto;Kazuhiko Nakao;Gregory Gores
• 通讯作者: Gregory Gores

PalmitoleateはERストレスの軽減によりPalmitateによる肝細胞アポトーシスを抑制する

棕榈油酸通过减少内质网应激抑制棕榈酸诱导的肝细胞凋亡

• 发表时间: 2011
• 作者: Lay-Myint Yoshida;Hien-Anh Nguyen;Kiwao Watanabe;Minh Nhat Le;Ai Thi Thuy Nguyen;Huong Thi Thu Vu;Hiroshi Yoshino;Motoi Suzuki;Kensuke Takahashi;Tho Huu Le;Paul E. Kilgore;Karen Edmond;Kim Mulholland;Duc-Anh Dang;Koya Ariyoshi;赤澤祐子
• 通讯作者: 赤澤祐子

Palmitoleate attenuates palmitate-induced Bim and PUMA up-regulation and hepatocyte lipoapoptosis Palmitoleate attenuates palmitate-induced Bim and PUMA up-regulation and hepatocyte lipoapoptosis

棕榈油酸减弱棕榈酸诱导的 Bim 和 PUMA 上调和肝细胞脂肪凋亡 棕榈油酸减弱棕榈酸诱导的 Bim 和 PUMA 上调和肝细胞脂肪凋亡

• 发表时间: 2010
• 期刊: Journal of hepatology
• 影响因子: 25.7
• 作者: Akazawa Y;Cazanave S;Mott JL;Elmi N;Bronk SF;Kohno S;Charlton MR;Gores GJ.
• 通讯作者: Gores GJ.