Endoplasmic reticulum stress is involved in H. Pylori associated gastritis

内质网应激与幽门螺杆菌相关性胃炎有关

• 批准号: 22890138
• 负责人: AKAZAWA Yuuko
• 金额: $ 1.81万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22890138/
• 关键词: VacA; ヘリコバクターピロリ; BH3オンリー蛋白; アポトーシス; ER stress; ヘリコバクター・ピロリ

Apoptosis by H. pylori vactuolating cytotoxin(VacA) plays a role in H. Pylori induced apoptosis in gastric epitherial cells. However, precise mechanism of VacA apoptosis is not understood. Our aim was to examine involvement of ER stress and pro-apoptotic BH3 only proteins in human H. Pylori infection and in VacA treated cells. GADD34 mRNA and GRP 78 mRNA were significantly increased in H. Pylori infected gastric mucosa. Immunohistochemistry showed CRP78 staining in gastric epitherial cells. Apoptosis inducing ER stress marker CHOP was significantly elevated in AZ521 treated with VacA, and was contributing to apoptosis. The present study is, to our knowledge, among the first to implicate the association of ER stress with human H. Pylori infection and its apoptosis inducing toxin, VacA.

幽门螺杆菌的凋亡凋亡细胞毒素（VACA）在幽门螺杆菌诱导的胃内膜细胞中凋亡中起作用。但是，尚不了解VACA凋亡的精确机制。我们的目的是检查幽门螺杆菌感染和VACA处理的细胞中仅蛋白质的ER应激和促凋亡BH3的参与。幽门螺杆菌感染的胃粘膜中GADD34 mRNA和GRP 78 mRNA显着增加。免疫组织化学显示在胃内层细胞中染色CRP78。在用VACA处理的AZ521中，诱导ER应力标记的凋亡显着升高，并有助于凋亡。据我们所知，本研究是第一个暗示ER应激与幽门螺杆菌感染及其诱导毒素毒素的凋亡的关联的研究。

项目成果

The Mechanisms by Which Unsaturated free fatty Acid Attenuates Saturated Free Fatty Acid Mediated Apoptosis

不饱和游离脂肪酸减弱饱和游离脂肪酸介导的细胞凋亡的机制

• 发表时间: 2010
• 作者: Yuko Akazawa;Hajime Isomoto;Kazuhiko Nakao;Gregory Gores
• 通讯作者: Gregory Gores

Palmitoleate attenuates palmitate-induced Bim and PUMA up-regulation and hepatocyte lipoapoptosis Palmitoleate attenuates palmitate-induced Bim and PUMA up-regulation and hepatocyte lipoapoptosis

棕榈油酸减弱棕榈酸诱导的 Bim 和 PUMA 上调和肝细胞脂肪凋亡 棕榈油酸减弱棕榈酸诱导的 Bim 和 PUMA 上调和肝细胞脂肪凋亡

• 发表时间: 2010
• 期刊: Journal of hepatology
• 影响因子: 25.7
• 作者: Akazawa Y;Cazanave S;Mott JL;Elmi N;Bronk SF;Kohno S;Charlton MR;Gores GJ.
• 通讯作者: Gores GJ.

PalmitoleateはERストレスの軽減によりPalmitateによる肝細胞アポトーシスを抑制する

棕榈油酸通过减少内质网应激抑制棕榈酸诱导的肝细胞凋亡

• 发表时间: 2011
• 作者: Lay-Myint Yoshida;Hien-Anh Nguyen;Kiwao Watanabe;Minh Nhat Le;Ai Thi Thuy Nguyen;Huong Thi Thu Vu;Hiroshi Yoshino;Motoi Suzuki;Kensuke Takahashi;Tho Huu Le;Paul E. Kilgore;Karen Edmond;Kim Mulholland;Duc-Anh Dang;Koya Ariyoshi;赤澤祐子
• 通讯作者: 赤澤祐子