Atomic details of antimicrobial peptides at work in live cells

抗菌肽在活细胞中发挥作用的原子细节

• 批准号: DP160100959
• 负责人: Prof Frances Separovic
• 金额: $ 26.47万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-01-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP160100959
• 关键词: Atomic; details; antimicrobial; peptides; work

This project aims to develop methods to determine the detailed structure of biologically important molecules in live cells to better understand how biomolecular structure is related to disease. The structure at the atomic level of the molecules of life is usually characterised by crystal or solution studies in model systems. However, the structure of many biologically important molecules depends on their environment. Using new instrumentation and labelling schemes, the project plans to use nuclear magnetic resonance methods to study antibiotics and antimicrobial peptides in live bacteria and human cells. The goal is to resolve how these molecules cross cell membranes and how biomolecular structure is related to activity, which may advance development in biotechnology and therapeutic treatments against drug-resistant infections.

该项目旨在开发确定活细胞中生物学重要分子的详细结构的方法，以更好地了解生物分子结构与疾病的关系。生命分子在原子水平上的结构通常通过模型系统中的晶体或溶液研究来表征。然而，许多生物重要分子的结构取决于它们的环境。该项目计划使用新的仪器和标签方案，利用核磁共振方法研究活细菌和人类细胞中的抗生素和抗菌肽。目标是解决这些分子如何穿过细胞膜以及生物分子结构如何与活性相关，这可能会推动生物技术和耐药感染治疗的发展。