Research of nitrogen metabolism controlling system in insects

昆虫氮代谢控制系统的研究

• 批准号: 23880014
• 负责人: YOSHINAGA Naoko
• 金额: $ 1.5万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23880014/
• 关键词: 鱗翅目幼虫; FACs; 窒素代謝; アミノ酸; 腸管; Orbitrap

Fatty acid amino acid conjugates (FACs) in lepidopteran caterpillars enhance their nitrogen assimilation and are considered to accelerate their growth ratio. The research focused on the biosynthetic mechanism of FACs in lepidopteran caterpillars.For the first time, in vitro biosynthesis of glutamate conjugates were observed when the substrates were incubated with a midgut tissue of Spodoptera litura, silkworm, tobacco hornworm and a few other species. That means glutamate is directly conjugated with fatty acid in lepidopteran caterpillars, suggesting the synthetic routeis the same with the case of fruit fly Drosophila melanogaster but clearly different from the case of crickets Gryllus emma. Crickets first conjugate glutamine with fatty acid and modify the side chain of glutamine moiety to make a glutamate conjugate. Although FACs in these insects share a common ground such as localization in themidgut tissues and the same analog pattern consisting of glutamine/glutamate, the finding suggest that at least glutamate type FACs were independently developed in these insect order.

鳞翅目毛虫体内的脂肪酸氨基酸结合物(FAC)可促进其氮素同化，促进其生长发育。本研究以鳞翅目毛虫为研究对象，首次将其与斜纹夜蛾、家蚕、烟草角虫等几种昆虫的中肠组织孵育，在体外观察到谷氨酸结合物的生物合成。这意味着在鳞翅目毛虫中，谷氨酸直接与脂肪酸结合，这表明合成路线与果蝇的情况相同，但与蟋蟀的情况明显不同。蟋蟀首先将谷氨酰胺与脂肪酸偶联，然后修饰谷氨酰胺部分的侧链，形成谷氨酸偶联物。虽然这些昆虫的FAC有一个共同点，如定位于中肠组织，以及相同的由谷氨酰胺/谷氨酸组成的类似模式，但这一发现表明，至少谷氨酸型FAC在这些昆虫中是独立发展的。

项目成果

Biosynthesis of plant volatile eliciting FACs in lepidopteran caterpillars, fruit flies and crickets: Convergent evolution or phylogenetic inheritance?

鳞翅目毛虫、果蝇和蟋蟀中植物挥发物的生物合成引发 FAC：趋同进化还是系统发育遗传？

• 发表时间: 2012
• 作者: Naoko Yoshinaga;et al.
• 通讯作者: et al.

鱗翅目幼虫腸管におけるヒドロキシ脂肪酸

鳞翅目幼虫肠道中的羟基脂肪酸

• 发表时间: 2012
• 作者: 村上真一郎;網干貴子;吉永直子;小野肇;西田律夫;森直樹
• 通讯作者: 森直樹

鱗翅目幼虫腸管内エリシターFACsの多様性から見る寄主植物-植食性昆虫相互作用の研究

从鳞翅目幼虫肠道激发子FAC多样性角度研究宿主植物-草食昆虫相互作用

• 发表时间: 2012
• 作者: 吉永直子;森田沙代;網干貴子;西田律夫;森直樹;James H.Tumlinson
• 通讯作者: James H.Tumlinson

Identification of biosynthesis of fatty acid amino acid conjugates in Drodophila melanogaster larvae and adults

黑腹果蝇幼虫和成虫脂肪酸氨基酸缀合物生物合成的鉴定

• 发表时间: 2011
• 作者: T.Yoshida;T.Aboshi;N.Yoshinaga;M.Fukui;R.Nishida
• 通讯作者: R.Nishida

京都大学農学研究科応用生命科学専攻 化学生態学研究室

京都大学研究生院农学研究科应用生命科学系化学生态学实验室