Analysis of primordial germ cells migration for infertility in Japanese flounder

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In marine farmed fishes, it is not yet investigated the molecular mechanism in fundamental dynamic state of primordial germ cell PGC. Then, in this study, migration of PGC and the molecular mechanism were analyzed using the Japanese flounder. The cxcr4 (4 type chemokine receptor) gene is isolated in Japanese flounder, which is known that the involved with migration of a primordial germ cell.Moreover, I tried prevention of PGC migration by using a chemokine receptor inhibitor AMD3100 which combines with cxcr4 specifically and inhibit the work antagonistically. As a result, in the individual which administrated AMD3100, a PGC marker vasa mRNA is disappeared in Japanese flounder. It became clear from this result that cxcr4 participates in migration of PGC in theflounder.