Analysis of primordial germ cells migration for infertility in Japanese flounder

在海洋养殖鱼类中，原始生殖细胞PGC基本动态的分子机制尚未研究。本研究以牙鲆为研究对象，分析了PGC的迁移及其分子机制。从牙鲆中分离到cxcr4（4型趋化因子受体）基因，该基因参与了原始生殖细胞的迁移。此外，我尝试使用趋化因子受体抑制剂AMD3100与cxcr4特异性结合，拮抗PGC迁移。结果表明，注射AMD3100后，牙鲆体内PGC标记vasa mRNA消失。结果表明，cxcr4参与了PGC在比目鱼体内的迁移。

In marine farmed fishes, it is not yet investigated the molecular mechanism in fundamental dynamic state of primordial germ cell PGC. Then, in this study, migration of PGC and the molecular mechanism were analyzed using the Japanese flounder. The cxcr4 (4 type chemokine receptor) gene is isolated in Japanese flounder, which is known that the involved with migration of a primordial germ cell.Moreover, I tried prevention of PGC migration by using a chemokine receptor inhibitor AMD3100 which combines with cxcr4 specifically and inhibit the work antagonistically. As a result, in the individual which administrated AMD3100, a PGC marker vasa mRNA is disappeared in Japanese flounder. It became clear from this result that cxcr4 participates in migration of PGC in theflounder.