Relationship between lung metastases of osteosarcoma and coagulation-fibrinolytic factors

利用人骨肉瘤细胞系“143 B双色”，其中GFP在细胞核中表达，RFP在细胞质中表达，我们首先开发了具有多种转移潜能的多个细胞系。在每个细胞系中，我们通过ELISA和PCR评估uPA、派-1和TF的表达水平。结果显示uPA和派-1的表达与肿瘤转移潜能密切相关。我们已经成功地将uAP和派-1的shRNA转染到143 B细胞中。我们将探讨抑制uPA和派-1对骨肉瘤转移潜能的影响。

With use of human osteosarcoma cell line “143B dual color”, in which GFP is expressing in nucleus and RFP is expressing in cytoplasm, we first developed multiple cell lines that have multiple metastatic potential. In each cell line, we evaluated expression level of uPA, PAI-1, and TF by ELIZA and PCR. The result showed strong relationship between metastatic potential and expression of uPA & PAI-1. We have now performed shRNA of uAP & PAI-1 for 143B cell line. Then we will evaluate how metastatic potential change after suppression of uPA and PAI-1 in osteosarcoma.