Relationship between lung metastases of osteosarcoma and coagulation-fibrinolytic factors

骨肉瘤肺转移与凝血纤溶因子的关系

• 批准号: 23890070
• 负责人: KIMURA Hiroaki
• 金额: $ 2万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23890070/
• 关键词: 骨肉腫; 肺転移; プラスミノーゲン活性化因子インヒビタ-1 (PAI-1); ウロキナーゼ型プラスミノ-ゲン活性化因子; 蛍光蛋白; uPA; PAI-1; 凝固・線溶因子

With use of human osteosarcoma cell line “143B dual color”, in which GFP is expressing in nucleus and RFP is expressing in cytoplasm, we first developed multiple cell lines that have multiple metastatic potential. In each cell line, we evaluated expression level of uPA, PAI-1, and TF by ELIZA and PCR. The result showed strong relationship between metastatic potential and expression of uPA & PAI-1. We have now performed shRNA of uAP & PAI-1 for 143B cell line. Then we will evaluate how metastatic potential change after suppression of uPA and PAI-1 in osteosarcoma.

利用人骨肉瘤细胞系“143 B双色”，其中GFP在细胞核中表达，RFP在细胞质中表达，我们首先开发了具有多种转移潜能的多个细胞系。在每个细胞系中，我们通过ELISA和PCR评估uPA、派-1和TF的表达水平。结果显示uPA和派-1的表达与肿瘤转移潜能密切相关。我们已经成功地将uAP和派-1的shRNA转染到143 B细胞中。我们将探讨抑制uPA和派-1对骨肉瘤转移潜能的影响。

项目成果

Real time imaging of single sarcoma cell dynmics of lung metastasis

肺转移单个肉瘤细胞动态的实时成像

• 发表时间: 2012
• 作者: Murata N;Yokogawa H;Kobayashi A;Yamazaki N;Sugiyama K;Kimura H
• 通讯作者: Kimura H

FISHを用いた軟部肉腫におけるMDM2遺伝子増幅の解析

FISH 分析软组织肉瘤中 MDM2 基因扩增

• 发表时间: 2012
• 作者: Sumi;K.;Miyamoto;Y.;Ishikawa;Y.,Iegaki;N.;Muramatsu;S.;Hibi;Y.;Nabeshima;T.;Uno;K.;Nitta;A.;木村浩明
• 通讯作者: 木村浩明

Anti-β1 Integrin Antibody Inhibits Osteosarcoma Lung Seeding and Metastasis

抗 β1 整合素抗体抑制骨肉瘤肺种植和转移

• 发表时间: 2012
• 作者: Takaoka;K.;Uno;K.;Inagaki;R.;Nagakura;M.;Tamaji;A.;Ozaki;N.;Miyamoto;Y.;Nitta;A.;Kimura H
• 通讯作者: Kimura H

Caffeine induces apoptosis of osteosarcoma cells by inhibiting AKT/mTOR/S6K, NF-κB and MAPK pathways.

• 发表时间: 2012-09
• 期刊: Anticancer research
• 影响因子: 2
• 作者: S. Miwa;N. Sugimoto;N. Yamamoto;T. Shirai;H. Nishida;K. Hayashi;H. Kimura;A. Takeuchi;K. Igarashi

Long-term follow-up of cannulation for solitary bone cysts

孤立性骨囊肿插管的长期随访

• 发表时间: 2011
• 作者: Nitta;A.;Ishikawa;Y.;Iegaki;N.;Muramatsu;S.;Nabeshima;T.;Furukawa-Hibi;Y.;Uno;K.;Miyamoto;Y.;Kimura H
• 通讯作者: Kimura H