Relationship between lung metastases of osteosarcoma and coagulation-fibrinolytic factors

利用绿色荧光蛋白表达于细胞核，绿色荧光蛋白表达于细胞浆的人骨肉瘤细胞系“143B双色”，我们首次建立了具有多转移潜能的多细胞系。采用ELIZA法和聚合酶链式反应(PCR)检测各细胞系uPA、PAI-1和TF的表达水平。结果表明，uPA和PAI-1的表达与肿瘤的转移潜能密切相关。我们现在已经对143B细胞系进行了UAP和PAI-1的shRNA。然后我们将评估骨肉瘤中uPA和PAI-1抑制后转移潜能的变化。

With use of human osteosarcoma cell line “143B dual color”, in which GFP is expressing in nucleus and RFP is expressing in cytoplasm, we first developed multiple cell lines that have multiple metastatic potential. In each cell line, we evaluated expression level of uPA, PAI-1, and TF by ELIZA and PCR. The result showed strong relationship between metastatic potential and expression of uPA & PAI-1. We have now performed shRNA of uAP & PAI-1 for 143B cell line. Then we will evaluate how metastatic potential change after suppression of uPA and PAI-1 in osteosarcoma.