Analysis of a novel membrane estrogen receptor signaling and identification of its gene

大多数雌激素的作用是由经典的核受体介导的，这种核受体被称为基因组作用，调节各种基因的表达。近年来，膜受体介导的雌激素效应已被证实，本研究试图分析一种新的膜型雌激素受体在牛肾上腺髓质细胞中的信号转导机制，并克隆该受体基因。虽然已知的受体激活会导致细胞内钙和cAMP的增加，但雌激素并不能刺激牛肾上腺髓质细胞的这两种反应。这些结果表明，新受体的信号通路与已知的雌激素膜受体完全不同。为了探索受体基因，用牛肾上腺髓质细胞膜部分的多克隆抗体对牛肾上腺髓质细胞的mRNA噬菌体文库进行了淘洗。结果，我发现了几个有趣的基因。

Most of estrogen actions are mediated by classic nuclear receptor, which called genomic action and regulate expression of various genes. Recently, membrane receptor mediated effects of estrogen have been identified.In this study, I tried to analysis of a novel membrane type estrogen receptor signaling in bovine adrenal medullary cells and to cloning the receptor gene. Although, activation of already known receptors leads to increase of intracellular calcium and cAMP, estrogen did not stimulate both reactions in bovine adrenal medullary cells. These results indicated that signaling pathway of the novel receptor is completely different from the known estrogen membrane receptors. To explore the receptor gene, phage cDNA library made from mRNA of bovine adrenal medullary cells was subjected to panning with polyclonal antibody against cell membrane fraction of bovine adrenal medulla. As a result, I identified several interesting genes.