Development of Analysis Method of Immune Surveillance System in Various Animals by using Adenovirus Vector.

利用腺病毒载体开发各种动物免疫监视系统的分析方法。

• 批准号: 09556066
• 负责人: KAI Chieko
• 金额: $ 8.06万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-09556066/
• 关键词: adenovirus; canine distemper; CDV; H protein; CTL; アデノイルス; 牛疫; 細胞性免疫; ウシ白血病ウイルス; MHC; 牛疫ウイルス; ウサギ; 細胞傷害試験; 組み換えアデノウイルス; lacZ; 牛白血病ウイルス; ウシ

The AIM of this research is the development of analysis method of immune surveillance system in various animals by using adenovirus vector to characterize cell mediated immunity surveillance system. The various results were obtained, as followings,(1) We established the recombinant adenovirus vector encoding canine distemper virus (CDV) hemagglutinin (H) gene. We also established the method to get primary dog skin cells for the target of cytotoxic T lymphocyte (CTL) assay. It makes us possible to get the targeted cells from self-animals.(2) We identified epitopes for cell-mediated immunity and humoral immunity in N protein of rinderpest virus. By utilizing immune-stimulating complex (ISCOM), recombinant H protein was revealed to be effective in cell mediated immunity.(3) We identified the resistant Major Histocompatibility Complex (BoLA) class II molecules to the bovine leukemia ; BoLA-DR3 allele. The recombinant adenovirus encoding BLV genome

本研究的目的是利用腺病毒载体来表征细胞介导的免疫监视系统，建立各种动物免疫监视系统的分析方法。主要研究结果如下：（1）构建了犬瘟热病毒（CDV）血凝素（H）基因重组腺病毒载体。建立了犬皮肤细胞的原代培养方法，为细胞毒性T淋巴细胞（CTL）检测提供了实验依据。它使我们有可能从自体动物中获得靶细胞。(2)我们确定了牛瘟病毒N蛋白的细胞免疫和体液免疫表位。利用免疫刺激复合物（ISCOM），重组H蛋白被证明是有效的细胞介导的免疫。(3)我们鉴定了对牛白血病具有抗性的主要组织相容性复合物（BoLA）II类分子; BoLA-DR 3等位基因。编码BLV基因组的重组腺病毒

项目成果

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Ohashi,K. 等人：“从日本貉 (Nyctereutes procyonoides viverrinus) 中分离出的新 CDV 的特性。”兽医记录。

Iwatsuki,K., et al.: "Establishment of a persistent mutant of canine distemper virus."Microbe.Infect.. 1. 987-991 (1999)

Iwatsuki,K., et al.：“犬瘟热病毒持久突变体的建立。”Microbe.Infect.. 1. 987-991 (1999)

Nishino, et al.: "Human immunodeficiency virus type 1 vpr gene product prevents cell proliferation on mouse NIH3T3 cells without G2 arrest of the cell cycle."Biochem.Biophys.Res.Commun.. 232. 550-554 (1997)

Nishino 等人：“人类免疫缺陷病毒 1 型 vpr 基因产物可防止小鼠 NIH3T3 细胞上的细胞增殖，而无需细胞周期的 G2 停滞。”Biochem.Biophys.Res.Commun. 232. 550-554 (1997)

Nishizawa,M., et al.: "Induction of apoptosis by the Vpr protein of human immunodeficiency virus type 1 occurs independently of G2 arrest of the cell cycle."Virology. 276. 16-26 (2000)

Nishizawa,M., et al.：“1 型人类免疫缺陷病毒的 Vpr 蛋白诱导细胞凋亡的发生独立于细胞周期的 G2 停滞。”病毒学。

Shin, Y.-S., et al.: "Expression of the nucleocapsid protein gene of the canine distemper virus."J.Vet.Med.Sci.. 59(1). 51-53 (1997)

Shin, Y.-S., 等人：“犬瘟热病毒核衣壳蛋白基因的表达。”J.Vet.Med.Sci. 59(1)。