权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

MECHANISMS OF HOSTSPECIFICITY OF CANINE DISTEMPER VIRUS

犬瘟热病毒宿主特异性机制

基本信息

批准号：
09460136
负责人：
KAI Chieko
金额：
$ 8.45万
依托单位：
UNIVERSITY OF TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09460136/
关键词：
distemper virus canine wild animals Haemagglutinin CDV RFLP reverse genetics リバースジェネティックスアザラシタヌキルシフェラーゼ H protein F protein 野外株モノクローナル抗体

项目摘要

Recently, the incidence of canine distemper (CDV) in domestic dogs has increased in the world. We isolated numbers of viruses from affected dogs. Genetic analysis of the Haemagglutinin (H) proteins revealed that the recent isolated strains have an increased number of potential sites for glycosylation. We also revealed that the prevalence of the disease in Japan mainly caused by new types of CDV which could be distinguished from vaccines and old types of CDV by restriction fragment length polymorphism of H genes. By the analyses of cross-reactivity to monoclonal antibodies, at least one neutralizing epitope of the H protein of prevalent viruses was suggested to be changed. The full genome of the Yanaka strain, a new isolate was sequenced. It contains 15,690bp of nucleotides and deduced amino acid sequence had approximately 92% of homology compared with that of vaccine strain. The reverse genetics of Mononegavirales is an innovative technique for the rescue of infectious viruses. We conducted to develop this system on CDV and succeeded to recover recombinant CDV from the cDNA of CDV with unique restriction enzyme sites between structural genes for further basic and applied studies. On the other hand, we conducted the epidemiological survey of morbilliviruses on aquatic animals in the coast of Japan. Serological analysis of more than 300 animals including cetaceans and pinnipeds indicated that the pinnipeds appeared to have been infected with PDV-like virus before 1988 and cetaceans with DMV-like 1978 along the coast of Japan. The genetic and serological analyses of a newly isolated CDV from a raccoon dog in Japan revealed that only four differences in amino acids sequence of H protein indicating that the virus spreading in Japanese raccoon dogs belongs to the same group as the CDV strains causing recent outbreaks in dogs.

近年来，犬瘟热（CDV）在世界范围内家犬中的发病率呈上升趋势。我们从患病的狗身上分离出了大量的病毒。血凝素（H）蛋白的遗传分析显示，最近分离的菌株具有增加的潜在糖基化位点。我们还发现，这种疾病在日本的流行主要是由新型CDV引起的，通过H基因的限制性片段长度多态性可以将其与疫苗和旧型CDV区分开来。通过与单克隆抗体的交叉反应性分析，提示流行病毒H蛋白至少有一个中和表位发生了改变。对一株新分离株Yanaka株的全基因组进行了测序。其核苷酸序列为15，690bp，推导的氨基酸序列与疫苗株同源性约为92%。单负基因病毒目的反向遗传学是一种拯救感染性病毒的创新技术。本研究以犬瘟热病毒为研究对象，建立了一套重组犬瘟热病毒表达系统，并成功地从犬瘟热病毒的cDNA中回收了重组犬瘟热病毒，为进一步的基础和应用研究奠定了基础。另一方面，我们在日本沿海的水生动物上进行了麻疹病毒的流行病学调查。对包括鲸类和鳍足类在内的300多只动物进行的血清学分析表明，在日本沿海，鳍足类动物似乎在1988年之前感染了PDV样病毒，鲸类动物沿着在1978年感染了DMV样病毒。从日本一只浣熊犬中分离到的犬瘟热病毒的遗传学和血清学分析表明，在H蛋白的氨基酸序列上只有4个差异，表明在日本浣熊犬中传播的病毒与最近引起犬中暴发的犬瘟热病毒株属于同一组。