Analysis of the species specific pathogenicity of morbillivirus by reverse genetics.

新出现和再次出现的病毒感染单一病毒构成了一个全球性的社会问题，而超过被认为是影响因素的动物物种的感染机制的阐明是一个重大命题。近年来，犬瘟热病毒(CDV)导致2万只海豹和1000头狮子死亡。针对这一问题，我们从流行病学调查、病毒分离、基因分析、抗体制备、动物实验等方面做了大量的基础研究。此外，尽管牛瘟病毒具有临界致病性，但在奶牛身上进行感染实验并不容易，致病机制尚未解决。为了解决这一基本命题，我们建立了极为优良的兔动物模型实验系统，并对其致病性进行了详细的分析。具有这些背景的群体在世界上也没有榜样。虽然反向遗传系统是由单色Negat…建立的最近病毒更多了，这项新技术在世界上也只有很少的团队。我们成功地利用CDV和Rinderpest病毒(RPV)开发了这项新技术。此外，关于麻疹病毒(麻疹病毒；MV)，它是用原始的野生株建立的。在这些基础研究的基础上，我们开始了对病毒致病性和物种特异性的研究。具体地说，进行了以下研究项目：(1)病毒种特异性分析(2)CDV受体的鉴定(3)病毒致病性分析(4)参与病毒增殖的宿主细胞因子的分析我们可以得到许多结果，包括不同于预期的结果，这些结果指向关于麻疹病毒的物种特异性致病性的大命题。特别是关于(1)和(4)，表明P蛋白与RPVRBOK砧木和L砧木种子的致病力密切相关。此外，N蛋白作为控制L群体致病力的蛋白质，其可能性也变得明显。此外，(2)，关于(3)，与CDV感染有关的新分子的出现，并结合此对H蛋白的中和表位进行了分析。此外，还获得了带有报告基因的CDV，并阐明了感染易感细胞的鉴定和干预安排的重要性。因此，通过这项研究，可以获得许多对基础研究也很重要的知识，人们认为这项研究的贡献是很大的。较少

Emerging and reemerging virus infection by the mono-nega virus pose a social problem globally, and the elucidation of the infection mechanism exceeding the animal species considered to be the factor is a big proposition. Canine distemper virus (CDV) made 20,000 seals and 1000 lion numbers die in recent years. We have done abundant fundamental researches from epidemiology investigation with virus isolation, a gene analysis, antibody production, and the animal experiment to this problem. Moreover, although rinderpest virus shows critical pathogenicity, an infection experiment with a cow is not easy and the mechanism of pathogenicity is not solved. In order to solve this fundamental proposition, we established the extremely excellent animal model experiment system of a rabbit, and have performed detailed analysis of the pathogenicity. The group with these backgrounds does not have an example in the world, either. Although the reverse genetics system was established by the monochrome negat … More ive virus recently, this new technology has only very few teams also in the world. We succeeded in development of this new technology by CDV and Rinderpest virus (RPV). Furthermore, about the measles virus (Measles virus; MV), it was established using the original wild strain. With those basic researches, we started the study on the viral pathogenicity and species specificity. Specifically, the following research item performed.(1) Analysis of viral species specificity (2) Identification of a CDV receptor (3) Analysis of viral pathogenicity (4) Analysis of a host cell factor which participates in virus multiplicationWe could obtain the many results including not also expected, and they direction to a big proposition about species-specific pathogenicity of the morbillivirus. Especially, about (1) and (4), it suggested that P protein was greatly concerned with the pathogenicity which exceeded the seed using the RBOK stock of RPV, and L stocks. Moreover, as protein which governs the pathogenicity of L stocks of inside, the possibility of N protein became clear. Moreover, (2), About (3), the new molecule in connection with infection of CDV surfaced, and the neutralization epitope on H protein combined with this was also analyzed. Moreover, CDV with the reporter gene was produced and identification of an infection susceptibility cell and importance of intervention arrangement were clarified. Thus, by this research, the knowledge of many important also for fundamental research could be acquired, and it was thought that the contribution of this research was large. Less