Analysis of the species specific pathogenicity of morbillivirus by reverse genetics.

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Emerging and reemerging virus infection by the mono-nega virus pose a social problem globally, and the elucidation of the infection mechanism exceeding the animal species considered to be the factor is a big proposition. Canine distemper virus (CDV) made 20,000 seals and 1000 lion numbers die in recent years. We have done abundant fundamental researches from epidemiology investigation with virus isolation, a gene analysis, antibody production, and the animal experiment to this problem. Moreover, although rinderpest virus shows critical pathogenicity, an infection experiment with a cow is not easy and the mechanism of pathogenicity is not solved. In order to solve this fundamental proposition, we established the extremely excellent animal model experiment system of a rabbit, and have performed detailed analysis of the pathogenicity. The group with these backgrounds does not have an example in the world, either. Although the reverse genetics system was established by the monochrome negat … More ive virus recently, this new technology has only very few teams also in the world. We succeeded in development of this new technology by CDV and Rinderpest virus (RPV). Furthermore, about the measles virus (Measles virus; MV), it was established using the original wild strain. With those basic researches, we started the study on the viral pathogenicity and species specificity. Specifically, the following research item performed.(1) Analysis of viral species specificity (2) Identification of a CDV receptor (3) Analysis of viral pathogenicity (4) Analysis of a host cell factor which participates in virus multiplicationWe could obtain the many results including not also expected, and they direction to a big proposition about species-specific pathogenicity of the morbillivirus. Especially, about (1) and (4), it suggested that P protein was greatly concerned with the pathogenicity which exceeded the seed using the RBOK stock of RPV, and L stocks. Moreover, as protein which governs the pathogenicity of L stocks of inside, the possibility of N protein became clear. Moreover, (2), About (3), the new molecule in connection with infection of CDV surfaced, and the neutralization epitope on H protein combined with this was also analyzed. Moreover, CDV with the reporter gene was produced and identification of an infection susceptibility cell and importance of intervention arrangement were clarified. Thus, by this research, the knowledge of many important also for fundamental research could be acquired, and it was thought that the contribution of this research was large. Less