Analysis of the species specific pathogenicity of morbillivirus by reverse genetics.

单胞病毒的新发和再发病毒感染是一个全球性的社会问题，对其感染机制的阐释超越了被认为是影响因素的动物物种是一个很大的课题。犬瘟热病毒（CDV）近年来造成2万头海豹和1000头狮子死亡。针对这一问题，我们从流行病学调查、病毒分离、基因分析、抗体生产、动物实验等方面做了大量的基础研究。此外，虽然牛瘟病毒表现出临界致病性，但在牛身上进行感染实验并不容易，其致病性机制也没有得到解决。为了解决这一基本命题，我们建立了极为完善的家兔动物模型实验体系，并对致病性进行了详细的分析。拥有这样背景的群体在世界上也没有榜样。虽然反向遗传系统是由单色阴性病毒建立的，但这种新技术在世界上也只有很少的团队。我们成功地开发了CDV和牛瘟病毒（RPV）的新技术。此外，麻疹病毒（麻疹病毒；MV）是利用原始野生株建立的。有了这些基础研究，我们开始了病毒致病性和物种特异性的研究。具体而言，进行了以下研究项目。(1)病毒的种特异性分析(2)一种CDV受体的鉴定(3)病毒的致病性分析(4)参与病毒增殖的宿主细胞因子的分析我们得到了许多意想不到的结果，这些结果指向了一个关于麻疹病毒种特异性致病性的大命题。特别是(1)和(4)表明，在RPV的RBOK砧木和L砧木中，P蛋白与致病性密切相关，其致病性超过了种子。此外，作为控制内源L库致病性的蛋白，N蛋白的可能性也变得清晰起来。此外，(2)、(3)，与CDV感染相关的新分子浮出水面，并分析了与之结合的H蛋白上的中和表位。制备了带有报告基因的CDV，并阐明了感染易感细胞的鉴定和干预安排的重要性。因此，通过这项研究，可以获得许多重要的基础研究知识，人们认为这项研究的贡献是巨大的。少

Emerging and reemerging virus infection by the mono-nega virus pose a social problem globally, and the elucidation of the infection mechanism exceeding the animal species considered to be the factor is a big proposition. Canine distemper virus (CDV) made 20,000 seals and 1000 lion numbers die in recent years. We have done abundant fundamental researches from epidemiology investigation with virus isolation, a gene analysis, antibody production, and the animal experiment to this problem. Moreover, although rinderpest virus shows critical pathogenicity, an infection experiment with a cow is not easy and the mechanism of pathogenicity is not solved. In order to solve this fundamental proposition, we established the extremely excellent animal model experiment system of a rabbit, and have performed detailed analysis of the pathogenicity. The group with these backgrounds does not have an example in the world, either. Although the reverse genetics system was established by the monochrome negat … More ive virus recently, this new technology has only very few teams also in the world. We succeeded in development of this new technology by CDV and Rinderpest virus (RPV). Furthermore, about the measles virus (Measles virus; MV), it was established using the original wild strain. With those basic researches, we started the study on the viral pathogenicity and species specificity. Specifically, the following research item performed.(1) Analysis of viral species specificity (2) Identification of a CDV receptor (3) Analysis of viral pathogenicity (4) Analysis of a host cell factor which participates in virus multiplicationWe could obtain the many results including not also expected, and they direction to a big proposition about species-specific pathogenicity of the morbillivirus. Especially, about (1) and (4), it suggested that P protein was greatly concerned with the pathogenicity which exceeded the seed using the RBOK stock of RPV, and L stocks. Moreover, as protein which governs the pathogenicity of L stocks of inside, the possibility of N protein became clear. Moreover, (2), About (3), the new molecule in connection with infection of CDV surfaced, and the neutralization epitope on H protein combined with this was also analyzed. Moreover, CDV with the reporter gene was produced and identification of an infection susceptibility cell and importance of intervention arrangement were clarified. Thus, by this research, the knowledge of many important also for fundamental research could be acquired, and it was thought that the contribution of this research was large. Less