Analysis of the species specific pathogenicity of morbillivirus by reverse genetics.

由单负病毒引起的新发和再发病毒感染在全球范围内构成了社会问题，并且阐明被认为是因素的动物物种之外的感染机制是一个大命题。犬瘟热病毒（CDV）近年来已造成2万只海豹和1000头狮子死亡。我们对该问题从流行病学调查、病毒分离、基因分析、抗体制备、动物实验等方面进行了大量的基础研究。此外，虽然牛瘟病毒显示出临界致病性，但用牛进行感染实验并不容易，并且致病性机制尚未解决。为了解决这一基本命题，我们建立了一个极为优良的家兔动物模型实验系统，并对其致病性进行了详细的分析。具有这些背景的群体在世界上也没有一个榜样。尽管反向遗传学系统是由单色染色体组建立的， 关于我们 最近，这项新技术在世界上也只有很少的几个团队。我们利用CDV和牛瘟病毒（RPV）成功地开发了这一新技术。此外，关于麻疹病毒（麻疹病毒; MV），使用原始野生株建立。在这些基础研究的基础上，我们开始了病毒致病性和种属特异性的研究。具体而言，进行了以下研究项目。(1)病毒种特异性分析（2）CDV受体的鉴定（3）病毒致病性分析（4）参与病毒增殖的宿主细胞因子的分析我们得到了许多意想不到的结果，它们为麻疹病毒种特异性致病性的研究提供了方向。特别是在（1）和（4）中，P蛋白与RPV的RBOK原种和L原种的致病力有很大关系，RPV的RBOK原种和L原种的致病力超过种子。此外，N蛋白作为控制L原种内致病性的蛋白质的可能性也变得清晰起来。此外，（2），关于（3），与CDV感染相关的新分子浮出水面，并且还分析了与此结合的H蛋白上的中和表位。此外，CDV与报告基因的生产和感染易感性细胞的鉴定和干预安排的重要性进行了澄清。因此，通过这项研究，可以获得许多对基础研究也很重要的知识，人们认为这项研究的贡献很大。少

Emerging and reemerging virus infection by the mono-nega virus pose a social problem globally, and the elucidation of the infection mechanism exceeding the animal species considered to be the factor is a big proposition. Canine distemper virus (CDV) made 20,000 seals and 1000 lion numbers die in recent years. We have done abundant fundamental researches from epidemiology investigation with virus isolation, a gene analysis, antibody production, and the animal experiment to this problem. Moreover, although rinderpest virus shows critical pathogenicity, an infection experiment with a cow is not easy and the mechanism of pathogenicity is not solved. In order to solve this fundamental proposition, we established the extremely excellent animal model experiment system of a rabbit, and have performed detailed analysis of the pathogenicity. The group with these backgrounds does not have an example in the world, either. Although the reverse genetics system was established by the monochrome negat … More ive virus recently, this new technology has only very few teams also in the world. We succeeded in development of this new technology by CDV and Rinderpest virus (RPV). Furthermore, about the measles virus (Measles virus; MV), it was established using the original wild strain. With those basic researches, we started the study on the viral pathogenicity and species specificity. Specifically, the following research item performed.(1) Analysis of viral species specificity (2) Identification of a CDV receptor (3) Analysis of viral pathogenicity (4) Analysis of a host cell factor which participates in virus multiplicationWe could obtain the many results including not also expected, and they direction to a big proposition about species-specific pathogenicity of the morbillivirus. Especially, about (1) and (4), it suggested that P protein was greatly concerned with the pathogenicity which exceeded the seed using the RBOK stock of RPV, and L stocks. Moreover, as protein which governs the pathogenicity of L stocks of inside, the possibility of N protein became clear. Moreover, (2), About (3), the new molecule in connection with infection of CDV surfaced, and the neutralization epitope on H protein combined with this was also analyzed. Moreover, CDV with the reporter gene was produced and identification of an infection susceptibility cell and importance of intervention arrangement were clarified. Thus, by this research, the knowledge of many important also for fundamental research could be acquired, and it was thought that the contribution of this research was large. Less