Development of Immune Induction System by Using The Novel Virus Vectors of Cytokines

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The purpose of this research is making cytokine using the recombinant SENDAI virus reverse genetics system which is a new recombinant protein production system, and examining the biological activity in in vitro and in vivo. Then, it is doing fundamental research of a cytokine network by checking activity, and using for development of the effective cytokine adjuvant immunity guiding method by medication of protein or a virus vector on the base.1) The recombination SENDAI virus which has cDNA of dog IFNγ, GM-CSF, IL-8, and IL6, respectively was able to be made, it was able to succeed in condensing and purification, and the products which show biological activity was able to be checked by in vitro. The dog was actually medicated with this fiscal year, and it searched about the validity in in vivo.2) GM-CSF refined from embryonated chicken eggs which inoculated the dog GM-CSF encoding SENDAI virus guided multiplication of a dog origin marrow cell or a GM-CSF dependability cell stock (TF-1 cell) to concentration dependability. When the healthy dog of six animals was medicated, the remarkable increase in peripheral leukocytes, neutrophiles and monocytes was accepted.3) When the skin pathological change part of a Leishmania protozoan infection mouse was medicated with dog IL-8 which were made to discover similarly and were refined over five days from the 14 days of infection, aggravation (expansion) of a skin pathological change was able to be controlled. Dog cytokine producing system by the recombination SENDAI virus have shown to be a powerful tool [having sufficient living thing activity also in in vivo from the above results.