The immune functions in aged cynomolgus monkeys as an experimental model.

以老年食蟹猴为实验模型的免疫功能。

• 批准号: 02680037
• 负责人: KAI Chieko
• 金额: $ 1.22万
• 依托单位: The university of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02680037/
• 关键词: aging; cynomolgus monkey; immune function; T lymphocyte; B lymphocyte; NL cell; macrophage

According to the increase in population of aged people, the immune status of aged humans is demanded to be studied to prevent them from disease's. We have been studied the immune system in aged cynomolgus monkeys to establish a model for clarifying the age-related change of immune functions in humans. The results obtained in 2 years were as follows and the cymonolgus monkey were considered to be very useful model to study the effect of aging.1)Since CH50 value and anti-A and anti-B activity levels reached the peak at 5 to 10 years of age and declined, the monkeys older than 20 years old are considered as aged monkeys.2)The subset labels of pant CD4+, CD8+ cells in cynomolgus monkeys increased according to aging although the ones of B and NK cells decreased.3)Interestingly relative high percentage of CD4+/CD8+ cells are observed in monkey peripheral blood in contrast to human and it increased with aging which suggest that the cynomolgus monkey is useful model to study the age-associated decline in thymus function and the differential mechanism of T cells.4)Comparing with young monkeys, autoantibodies are higher and T cell functions especially the productivity of IL-2 and the activity of IL-2R expression were considerably lower in aged monkeys. The NK activity and the phagocytic activity of macrophages were not changed with aging.5)Antibodies to recognize monkey B cells were obtained in anti-human B cells monoclonal and polyclonal antibodies.6)The assay system to study monkey B cell blastogenic activity was established.

随着老年人口的增加，需要对老年人的免疫状况进行研究，以预防老年人的疾病。我们研究了老年食蟹猴的免疫系统，以建立一个模型来阐明人类免疫功能的年龄相关性变化。2年内获得的结果如下，食蟹猴被认为是研究衰老效应的非常有用的模型。1）由于CH 50值和抗A和抗B活性水平在5至10岁时达到峰值并下降，因此20岁以上的猴被认为是老年猴。2）pant CD 4+，食蟹猴外周血中的CD 8+细胞随着年龄的增长而增加，而B细胞和NK细胞则随着年龄的增长而减少。3）有趣的是，与人相比，在猴外周血中观察到相对较高的CD 4 +/CD 8+细胞百分比，并且随着年龄的增长而增加，这表明食蟹猴是研究年龄-4）与青年猴相比，老年猴的自身抗体水平较高，T细胞功能尤其是IL-2产生能力和IL-2 R表达活性明显降低。NK活性和巨噬细胞的吞噬活性不随年龄的增长而变化。5）在抗人B细胞单克隆抗体和多克隆抗体中获得了识别猴B细胞的抗体。

项目成果

Kai,C.et al: "Monoclonal and polyclonal antibodies to human B crossーreacting to B cells of cynomolgus,green and rhurus monkys." J.Med.Primatol.

Kai, C. 等人：“人类 B 的单克隆和多克隆抗体与食蟹猴、绿猴和猕猴的 B 细胞发生交叉反应。”J.Med.Primatol。

Terao,K.et al: "Immune functions in aged cynomolgus monkeys.〔I〕 Hemolytic complement activity,natural antibody level,blastogenesis,natural killer activity and phagocytosis." Primatology today. 639-640 (1991)

Terao, K. 等人：“老年食蟹猴的免疫功能。[I] 溶血补体活性、天然抗体水平、胚芽​​发生、自然杀伤活性和吞噬作用。”《今日灵长类动物学》639-640。

Kai,C.et al: "Monocolnal and polyclonal antibodies to human B cross-reacting to B cells of cynomolgus,green and rherus monkeys." J.Med.Primatol.

Kai,C.等人：“人类 B 的单克隆和多克隆抗体与食蟹猴、绿猴和恒河猴的 B 细胞发生交叉反应。”

Terao,K.et al: "Effects of hyperlipaemic serum on interleukinー2(ILー2)production,ILー2 receptor expression,and T cell proliferation induced by ILー2 in cynomolgus monkeys." Jpn,J.Med,Sci.Biol.44. 17-28 (1991)

Terao, K. 等人：“高脂血症血清对食蟹猴中白介素 2 (IL-2) 产生、IL-2 受体表达以及 IL-2 诱导的 T 细胞增殖的影响”，J. Med，Sci。 .Biol.44。

Terao,K.and Fukase,Y.: "Effects of hyperlipaemic serum on interleukin-2(IL-2) production,IL-2 receptor expression, and T cell proliferation induced by IL-2 in cynomolgus monkeys." Jap.J.Med.Sci.Biol.44. 17-28 (1991)

Terao, K. 和 Fukase, Y.：“高脂血症血清对食蟹猴中白细胞介素 2 (IL-2) 产生、IL-2 受体表达以及 IL-2 诱导的 T 细胞增殖的影响。”