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Establishment of a propagation technique and ecological surveys for the protection of Rebun lady's slippers

礼文女拖鞋保护繁殖技术的建立及生态调查

基本信息

批准号：
09480136
负责人：
TANIGUCHI Hirokazu
金额：
$ 8.45万
依托单位：
Tochigi Junior College, Kokugakuin University (2000-2001)International Christian University (1997-1999)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 2000
项目状态：
已结题

项目摘要

About 100 flowers of Cypripedium macranthos var. rebunense were artificially polhnated annually. Pollination rate was 90%. From among the formed capsules 10 were sampled for immature seed culture, and the remainings were left for natural seed propagation. Immature seeds were cultured at ICU and NAIST, and seedlings were obtained. However, acclimation of some of these seedlings tried in 2000 was unsuccessful. Those kept in laboratories are still growing. In 2001 some of them were transplanted to the experiment field after cold-treatment New shoots are expected to emerge in the spring of 2002. The increase in the number of flowered plants among those propagated by natural seeding reached 18% within the preservation area of 70mm^2 in Rebun Island during the observation period. No new seedlings from seeds artificially sown under or outside of the periphery of mature plants have been identified yet. Seedling formation is usually observed under mature plants. For seed germination under natural environment seeding time and condition of water supply seem to have great influence. Seeds just prior to full maturation seem to have more germination potential. Comparative nucleotide sequence analysis of 10 Cypripedium species which grow in the northern hemisphere was made using chloroplast DNA to understand their phylogenetic relationship. C.calceolus found in Rebun Island and Kitami district of Hokkaido was demonstrated to be distinctly different from those in western Europe and China. It is least possible that C.calceolus in Rebun Island is a denizen from these regions as has been claimed. We consider that C.calceolus in Rebun Island should be treated as an endangered species

约100朵大花千金莲。每年人工授粉一次。授粉率达90%。从形成的胶囊中抽取10粒用于未成熟种子培养，剩下的用于自然种子繁殖。未成熟的种子在ICU和NAIST上培养，获得了幼苗。然而，2000年尝试的一些苗木的驯化并不成功。那些保存在实验室中的细胞仍在增长。2001年，部分试管苗经冷处理后移栽到试验田，预计2002年春季将出现新梢。在观察期间，在雷本岛70mm2的保存区内，自然繁殖的开花植物数量增加了18%。目前还没有从人工播种在成熟植株外围或外围的种子中发现新的幼苗。幼苗形成通常是在成熟植株下观察到的。对于自然环境下的种子萌发，播种期和供水条件似乎有很大的影响。接近完全成熟的种子似乎有更多的发芽潜力。利用叶绿体DNA对生长在北半球的10种塞浦路斯植物进行了比较核苷酸序列分析，以了解它们之间的系统发育关系。在北海道仁本岛和北上区发现的Calceolus与西欧和中国发现的C.calceolus有明显不同。最不可能的是，雷布恩岛的C.calceolus是来自这些地区的居民，正如所说的那样。我们认为，热本岛的石斑鱼应被视为濒危物种