Ecological research for the protection of rear prlants specis in Hokkaido gathered of DNA material.

从濒危保护植物名录中筛选出一级保护植物157种（单子叶植物43种、离瓣植物643种、合瓣植物51种）和二级保护植物269种（单子叶植物61种、离瓣植物104种、合瓣植物104种）进行了生态调查。主要的科是百合科（24种），兰科（61种），毛茛科（22种），虎耳草科（13种），蔷薇科（19种），豆科（13种），堇菜科（22种），杜鹃花科（13种）、报春花科（13种）、龙胆科（11种）、玄参科（19种）、桔梗科（11种）和菊科（39种）。到2005年，我们已经完成了280种的调查。调查包括用样方法计数生长植物和用目测法测量植物高度。根据DNA比较分析，证明在Rebun岛发现的Cypripedium calceoulus与欧洲、俄罗斯和中国的Cypripedium calceoulus不相同。两次调查旅行到库页岛，在那里本物种首次在远东亚洲记录，以收集样品进行比较。比较DNA分析结果表明，以叶绿体trnL-trnF基因的内含子区为探针，在100 ~ 200 bp的DNA序列中，未发现有C. calceolus的存在（美国可分为三组，A型（中国、俄罗斯），B型（欧洲）和C型（北海道礼文岛和北见区）。（北海道濒危保护植物的现状I）和包括DNA分析结果在内的Cypripedium调查（Cypripedium在北美、欧洲、Nadhodka和日本的生态调查）发表在最近一期的国学院大学学报上。

An ecological survey was conducted on 157 species of Level I (43 momocots, 643 choripetalous plants and 51 sympetalous plants) and 269 species of Level II (61 monocots, 104 choripetalous plants and 104 sympetalpous plants) selected from a list of endangered protected species. Major families are Liliacea (24 species), Orchidaceae (61 species), Ranunculaceae (22 speciers), Saxifragaceae (13 species), Rosaceae (19 species), Leguminocae (13 species), Violaceae (22 species), Ericaceae (13 species), Primulaceae (13 species), Gentianaceae (11 species), Scrophulariacae (19 species), Campanulaceae (11 species) and Asteaceae (39 species). We have completed the survey on 280 species of them by 2005. The survey included counting of growing plants by the quadrat method and plant height measurements by visual recordings.Cypripedium calceoulus found in Rebun Island was demonstrated to be not identical to those growing in Eurore, Russia and China on the basis of comparative DNA analysis. Two survey trips were made to Sakhalin where this species was first recorded in Fareast Asia in order to cllect samples for comparison. However, no C.calceolus was found on these trips.As the results of the comparative DNA analysis, using mainly the intron region of trnL-trnF chloroplast DNA as a probe, C.calceolus (us can be divided into three groups, Type A (China, Russia), Type B (Europe) and Type C (Rebun Island and Kitami district in Hokkaido).Detailed reports on the survey of endangered species (Present status of endangered protected plant species in Hokkaido I) and on a survey of cypripedium including the results of DNA analysis (An ecological survey on Cypripedium in North America, Europe, Nadhodka and Japan) were published in the recent issue of the Bulletin of kokugakuin university.