Ecological research for the protection of rear prlants specis in Hokkaido gathered of DNA material.

对濒危保护植物名录中的157种一级保护植物(单子叶植物43种、脉瓣植物643种、合瓣植物51种)和二级保护植物269种(单子叶植物61种、脉瓣植物104种、合瓣植物104种)进行了生态调查。主要科有百合科(24种)、兰科(61种)、毛茅科(22种)、虎耳草科(13种)、蔷薇科(19种)、豆科(13种)、紫罗兰科(22种)、杜鹃花科(13种)、报春花科(13种)、龙胆科(11种)、玄参科(19种)、钟形科(11种)和菊科(39种)。到2005年，我们已经完成了对其中280个物种的调查。调查采用样方计数和目测株高相结合的方法。通过比较DNA分析，证明在热本岛发现的钙瓣塞浦路斯与生长在俄罗斯欧罗尔和中国的不完全相同。为了收集样本进行比较，对库页岛进行了两次调查，这是该种在远东亚洲首次记录的地方。作为比较DNA分析的结果，主要以trnL-trnF叶绿体DNA内含子区域为探针，将Calceolus分为A型(俄罗斯中国)、B型(欧洲)和C型(北海道罗本岛和北海道北美区)。详细报告了濒危物种调查(北海道I期濒危保护植物现状)和塞浦路斯调查(北美、欧洲、塞浦路斯生态调查)的结果Nadhodka和日本)发表在最近一期的Kokugakuin大学公报上。

An ecological survey was conducted on 157 species of Level I (43 momocots, 643 choripetalous plants and 51 sympetalous plants) and 269 species of Level II (61 monocots, 104 choripetalous plants and 104 sympetalpous plants) selected from a list of endangered protected species. Major families are Liliacea (24 species), Orchidaceae (61 species), Ranunculaceae (22 speciers), Saxifragaceae (13 species), Rosaceae (19 species), Leguminocae (13 species), Violaceae (22 species), Ericaceae (13 species), Primulaceae (13 species), Gentianaceae (11 species), Scrophulariacae (19 species), Campanulaceae (11 species) and Asteaceae (39 species). We have completed the survey on 280 species of them by 2005. The survey included counting of growing plants by the quadrat method and plant height measurements by visual recordings.Cypripedium calceoulus found in Rebun Island was demonstrated to be not identical to those growing in Eurore, Russia and China on the basis of comparative DNA analysis. Two survey trips were made to Sakhalin where this species was first recorded in Fareast Asia in order to cllect samples for comparison. However, no C.calceolus was found on these trips.As the results of the comparative DNA analysis, using mainly the intron region of trnL-trnF chloroplast DNA as a probe, C.calceolus (us can be divided into three groups, Type A (China, Russia), Type B (Europe) and Type C (Rebun Island and Kitami district in Hokkaido).Detailed reports on the survey of endangered species (Present status of endangered protected plant species in Hokkaido I) and on a survey of cypripedium including the results of DNA analysis (An ecological survey on Cypripedium in North America, Europe, Nadhodka and Japan) were published in the recent issue of the Bulletin of kokugakuin university.