Ecological research for the protection of rear prlants specis in Hokkaido gathered of DNA material.

从濒危保护物种名录中选取了157种（单子叶植物43种，毛叶植物643种，共缘植物51种）和269种（单子叶植物61种，毛叶植物104种，共缘植物104种）进行了生态调查。主要科有百合科（24种）、兰科（61种）、毛茛科（22种）、沙盆草科（13种）、蔷薇科（19种）、豆科（13种）、堇菜科（22种）、Ericaceae（13种）、报春花科（13种）、龙胆科（11种）、玄武莲科（19种）、桔梗科（11种）和紫菀科（39种）。截至2005年，我们已完成对其中280个物种的调查。调查包括用样方法计数生长植物和用目测记录测量植物高度。在热本岛发现的calceoulus与生长在欧洲、俄罗斯和中国的塞浦路斯不相同。为了收集样本进行比较，对库页岛进行了两次调查，该物种在远东首次被记录下来。然而，在这些旅行中没有发现calceolus。主要以trnL-trnF叶绿体DNA内含子区为探针，对比DNA分析结果表明，calceolus （us）可分为a型（中国、俄罗斯）、B型（欧洲）和C型（北海道热本岛和北海道北上地区）3个类群。最近的《国学大学学报》发表了关于濒危物种调查（北海道濒危保护植物现状I）和包括DNA分析结果在内的塞浦路斯调查（北美、欧洲、纳德卡和日本的塞浦路斯生态调查）的详细报告。

An ecological survey was conducted on 157 species of Level I (43 momocots, 643 choripetalous plants and 51 sympetalous plants) and 269 species of Level II (61 monocots, 104 choripetalous plants and 104 sympetalpous plants) selected from a list of endangered protected species. Major families are Liliacea (24 species), Orchidaceae (61 species), Ranunculaceae (22 speciers), Saxifragaceae (13 species), Rosaceae (19 species), Leguminocae (13 species), Violaceae (22 species), Ericaceae (13 species), Primulaceae (13 species), Gentianaceae (11 species), Scrophulariacae (19 species), Campanulaceae (11 species) and Asteaceae (39 species). We have completed the survey on 280 species of them by 2005. The survey included counting of growing plants by the quadrat method and plant height measurements by visual recordings.Cypripedium calceoulus found in Rebun Island was demonstrated to be not identical to those growing in Eurore, Russia and China on the basis of comparative DNA analysis. Two survey trips were made to Sakhalin where this species was first recorded in Fareast Asia in order to cllect samples for comparison. However, no C.calceolus was found on these trips.As the results of the comparative DNA analysis, using mainly the intron region of trnL-trnF chloroplast DNA as a probe, C.calceolus (us can be divided into three groups, Type A (China, Russia), Type B (Europe) and Type C (Rebun Island and Kitami district in Hokkaido).Detailed reports on the survey of endangered species (Present status of endangered protected plant species in Hokkaido I) and on a survey of cypripedium including the results of DNA analysis (An ecological survey on Cypripedium in North America, Europe, Nadhodka and Japan) were published in the recent issue of the Bulletin of kokugakuin university.