Characterization of the cellular and molecular defects induced by the boron deficiency mimic phenylboronic acid in the primary root of maize

玉米初生根中缺硼模拟苯硼酸引起的细胞和分子缺陷的表征

• 批准号: 455453902
• 负责人: Dr. Michaela Matthes
• 金额: --
• 依托单位: Lehrstuhl für Crop Functional Genomics
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/455453902?language=en
• 关键词: Characterization; cellular; molecular; defects; induced

Deficiency of the micronutrient boron is a widespread abiotic stress leading to drastic yield reductions in many crops, including maize. The underlying molecular causes are only partially understood. Boron is accessible to plants in form of boric acid and the key in understanding boron function lies in its ability to bind to cis-diol groups. Although there are many potential binding partners of boron within a plant cell, it has only been shown to bind to and more importantly to crosslink two Rhamnogalacturonan-II molecules in the cell wall. Whether there are additional binding partners of boron and whether boron outside the cell wall is of biological importance are open questions in the field. To address these questions, the proposed project will make use of the chemical phenylboronic acid (PBA), which is a boric acid analog. Like boric acid, PBA still delivers boron into the cell and can bind to cis-diol groups. Unlike boric acid, PBA cannot cross link molecules and is therefore used to induce boron-deficiency like defects. PBA has the potential to identify additional functions of boron despite its crosslinking function and additional binding partners of boron. The proposed project will characterize the cellular and molecular defects induced by PBA in the maize primary root and will compare them to defects induced by boron deficiency. Special emphasis will be put on cell division, cell expansion, meristem development, ethylene biosynthesis, auxin transport and cytokinin signaling. Additionally, the proposed project will identify targets of PBA through forward chemical screening and proteomics approaches.

微量营养素硼缺乏是一种广泛存在的非生物胁迫，导致包括玉米在内的许多作物产量急剧下降。根本的分子原因仅被部分了解。硼可以硼酸的形式被植物吸收，了解硼功能的关键在于它与顺式二醇基团结合的能力。尽管植物细胞内有许多潜在的硼结合伴侣，但它仅被证明可以与细胞壁中的两个鼠李糖半乳糖醛酸-II 分子结合，更重要的是交联。是否存在额外的硼结合伴侣以及细胞壁外的硼是否具有生物学重要性是该领域的悬而未决的问题。为了解决这些问题，拟议的项目将使用化学苯硼酸（PBA），它是一种硼酸类似物。与硼酸一样，PBA 仍然将硼输送到细胞中，并可以与顺式二醇基团结合。与硼酸不同，PBA 不能交联分子，因此可用于诱发缺硼等缺陷。尽管 PBA 具有交联功能和硼的其他结合伙伴，但它仍具有识别硼的其他功能的潜力。拟议的项目将表征 PBA 在玉米初生根中引起的细胞和分子缺陷，并将其与缺硼引起的缺陷进行比较。将特别强调细胞分裂、细胞扩增、分生组织发育、乙烯生物合成、生长素运输和细胞分裂素信号传导。此外，拟议的项目将通过正向化学筛选和蛋白质组学方法确定 PBA 的靶点。