TISSUE ENGINEERING CARDIOVASCULAR STRUCTURES

组织工程心血管结构

基本信息

  • 批准号:
    10470279
  • 负责人:
  • 金额:
    $ 7.49万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
  • 财政年份:
    1998
  • 资助国家:
    日本
  • 起止时间:
    1998 至 2000
  • 项目状态:
    已结题

项目摘要

Various vascular grafts are commonly used in the reconstruction of cardiovascular tissues. However, currently used prosthetic or bioprosthetic materials lack growth potential and, therefore, subsequently require replacement in pediatric patients as they mature. Tissue engineering (TE) is a new discipline that offers the potential to create replacement structures from autologous cells and biodegradable polymer scaffolds. Because TE constructs contain living cells, they may have the potential to grow, self-repair, and self-remodel. Tissue Engineered Vascular Autografts (TEVAs) were made by seeding 4-6 x 10^6 of mixed cells obtained from femoral veins of mongrel dogs onto tube-shaped biodegradable polymer scaffolds composed of a polyglycolic acid (PGA) non-woven fabric sheet and a co-polymer of l-lactide and- caprolactone (N=4). After 7 days. the inferior vena cavas (IVCs) of the same dogs were replaced with TEVAs. After 3, 4, 5 and 6 months, angiographies were performed. and the dogs wer … More e sacrificed. The implanted TEVAs were examined both grossly and immunohistologically. The implanted TEVAs showed no evidence of stenosis or dilatation. No thrombus was found inside the TEVAs, even without any anticoagulation therapy. Remnants of the polymer scaffolds were not observed in all specimens, and the overall gross appearance appeared similar to that of native IVCs. Immunohistological staining revealed the presence of Factor VIII positive nucleated cells at the luminal surface of the TEVAs. In addition, lesions were observed where α-smooth muscle actin and desmin positive cells existed. Implanted TEVAs contained a sufficient amount of extracellular matrix, and showed neither occlusion nor aneurysmal formation. In addition, endothelial cells were found to line the luminal surface of each TEVA.These results strongly suggest that 'ideal' venous grafts with anti-thrombogenicity can be produced. Using the TE technique. a peripheral pulmonary artery was successfully reconstructed in a 4-year-old girl using autologous venous cells. The occluded pulmonary artery was reconstructed with the TE vessel graft. No postoperative complications occurred. On follow-up angiography, the transplanted vessels was noted to be completely patent. Long-term follow up is obviously necessary to confirm the feasibility of this approach. However. given our success, we now plan to continue to use this TE technique with autologous cells in only low pressure vessels, such as the venous or pulmonary circulation. Because both our laboratory and clinical experiences are quite encouraging, we suggest that the TE approach may play an important role as an alternative method to transplantation and to the use of artificial organs in the field of pediatric cardiovascular surgery. Less
各种血管移植物通常用于心血管组织的重建。然而,目前使用的假体或生物假体材料缺乏生长潜力,因此,随着其成熟,随后需要在儿科患者中进行更换。组织工程(TE)是一门新学科,它提供了从自体细胞和可生物降解的聚合物支架创建替代结构的潜力。由于TE构建体含有活细胞,因此它们可能具有生长、自我修复和自我重塑的潜力。组织工程血管自体移植物(TEVA)是通过将4-6 × 10^6个从杂种狗股静脉获得的混合细胞接种到管状生物可降解聚合物支架上制成的,该支架由聚乙醇酸(PGA)无纺布片和l-丙交酯和-己内酯的共聚物(N=4)组成。七天后。用TEVA替换相同狗的下腔静脉(IVC)。术后3、4、5、6个月行血管造影。狗狗们 ...更多信息 牺牲了。对植入的TEVA进行大体和免疫组织学检查。植入的TEVA未显示狭窄或扩张迹象。即使没有任何抗凝治疗,TEVA内也没有发现血栓。在所有样本中均未观察到聚合物支架的残留,总体外观与天然IVC相似。免疫组织学染色显示在TEVA的管腔表面存在因子VIII阳性有核细胞。此外,在α-平滑肌肌动蛋白和结蛋白阳性细胞存在的地方观察到病变。植入的TEVA含有足够量的细胞外基质,并且未显示闭塞或血管瘤形成。此外,内皮细胞被发现内衬每个TEVA的管腔表面。这些结果强烈地表明,“理想的”静脉移植物具有抗血栓形成可以产生。使用TE技术。使用自体静脉细胞在一个4岁女孩中成功地重建了外周肺动脉。闭塞的肺动脉用TE血管移植物重建。无术后并发症发生。在随访血管造影中,发现移植血管完全通畅。显然需要长期跟踪以确认这一方法的可行性。然而.鉴于我们的成功,我们现在计划继续使用这种TE技术,仅在低压血管中使用自体细胞,例如静脉或肺循环。由于我们的实验室和临床经验都非常令人鼓舞,我们建议TE方法作为移植和儿科心血管手术领域使用人造器官的替代方法可能发挥重要作用。少

项目成果

期刊论文数量(46)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Shinoka T, Ma PX, Shum-Tim D, Breuer CK, Cusick RA, Zund G, Langer R, Vacanti JP, Mayer JE, Jr.: "Tissue-engineered heart valves. Autologous valve leaflet replacement study in a lamb model."Circulation. 94. II164-168 (1996)
Shinoka T、Ma PX、Shum-Tim D、Breuer CK、Cusick RA、Zund G、Langer R、Vacanti JP、Mayer JE, Jr.:“组织工程心脏瓣膜。羔羊模型中的自体瓣叶替代研究。”
  • DOI:
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    0
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Shin'oka T, Yasuharu Imai, Manabu Watanabe, Goki Matsumura, Yoshimichi Kosaka, Takeshi Konuma, Narutoshi Hibino, Satoshi Toyama, Mitusnori Yamakawa, Yoshito Ikada, Shinichiro Morita: "Current Status of Tissue Engineering for Cardiovascular Structure"Journ
Shinoka T、Yasuharu Imai、Manabu Watanabe、Goki Matsumura、Yoshimichi Kosaka、Takeshi Konuma、Narutoshi Hibino、Satoshi Toyama、Mitusnori Yamakawa、Yoshito Ikada、Shinichiro Morita:“心血管结构组织工程的现状”杂志
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    0
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新岡俊治,今井康晴: "バイオ人工血管"医学のあゆみ. 195. 170-174 (2000)
Shunji Niioka,Yasuharu Imai:“生物人工血管”医学史 195. 170-174 (2000)。
  • DOI:
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    0
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新岡俊治: "再生医療の現場-「再生血管の移植」"実験医学. 18. 2276-2279 (2000)
新冈俊二:“再生医学领域-‘再生血管的移植’”《实验医学》18。2276-2279(2000)。
  • DOI:
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    0
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T Shinoka,Y Imai,M Watanabe,G Matsumura: "Current Status of Tissue Engineering for Cardiovascular Structure"Journal of Artificial Organ. 3. 102-106 (2000)
T Shinoka,Y Imai,M Watanabe,G Matsumura:“心血管结构组织工程的现状”人工器官杂志。
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    0
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IMAI Yasuharu其他文献

IMAI Yasuharu的其他文献

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{{ truncateString('IMAI Yasuharu', 18)}}的其他基金

A computerized control system for assisted circulation
辅助循环的计算机控制系统
  • 批准号:
    08457353
  • 财政年份:
    1996
  • 资助金额:
    $ 7.49万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of comuter controlled assisted circulation system
微机控制辅助循环系统的研制
  • 批准号:
    05671137
  • 财政年份:
    1993
  • 资助金额:
    $ 7.49万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
DEVELOPMENT OF COMPUTER CONTROLLED CARDIO-PULMONARY BYPASS SYSTEM
计算机控制心肺转流系统的研制
  • 批准号:
    62870054
  • 财政年份:
    1987
  • 资助金额:
    $ 7.49万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research
Experimental study of the low energy carbon dioxide laser for cardiovascular surgery
低能量二氧化碳激光心血管手术的实验研究
  • 批准号:
    60440066
  • 财政年份:
    1985
  • 资助金额:
    $ 7.49万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (A)

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    23H03730
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    2023
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