Molecular mechanism of regulation of methionine biosynthesis by mRNA stability : Studies using mto1 mutants of Arabidopsis.

通过 mRNA 稳定性调节蛋氨酸生物合成的分子机制:使用拟南芥 mto1 突变体进行的研究。

基本信息

  • 批准号:
    11440230
  • 负责人:
  • 金额:
    $ 10.69万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
  • 财政年份:
    1999
  • 资助国家:
    日本
  • 起止时间:
    1999 至 2000
  • 项目状态:
    已结题

项目摘要

Cystathionine gamma-synthase (CGS) of Arabidopsis has 563 amino acids and the first exon codes for 183 amino acids. Comparison of CGS sequence among higher plants reveals a 35 amino acid region ("MTO1 region") within the exon 1 that is highly conserved among plant species. This suggests that this region has a role in the regulation of CGS gene expression.Sequential deletion mutants within the CGS exon 1 was constructed from both the N-and C-termini. The deletion constructs were fused in-frame with a reporter gene, beta-glucuronidase (GUS), and placed under the control of cauliflower mosaic virus (CaMV) 35S RNA promoter and their response to Met application was analyzed by transient expression experiments. The results of GUS assay indicated that the constructs carrying the MTO1 region respond to Met application while those do not carry the MTO1 region do not. In addition, when mto1-1 mutation was introduced to those constructs that carry the MTO1 region, the response disappeared.Deletions in both sides of MTO1 were also constructed and tested for their response to Met application. The results suggest that the MTO1 region is sufficient for the downregulation in response to Met application.
拟南芥胱硫醚γ-合酶(CGS)基因全长563个氨基酸,第一外显子编码183个氨基酸。比较高等植物中的CGS序列揭示了外显子1内的35个氨基酸区域(“MTO 1区域”),其在植物物种中高度保守。从N端和C端构建了CGS外显子1的序列缺失突变体。将缺失构建体与报告基因β-葡萄糖醛酸酶(GUS)框内融合,并置于花椰菜花叶病毒(CaMV)35 S RNA启动子的控制下,通过瞬时表达实验分析它们对Met应用的响应。GUS检测结果表明,携带MTO 1区的构建体对Met的施用有应答,而不携带MTO 1区的构建体对Met的施用无应答。此外,当将mto 1 -1突变引入那些携带MTO 1区域的构建体时,反应消失。还构建了MTO 1两侧的缺失,并测试了它们对Met应用的反应。结果表明,MTO 1区域足以响应于Met应用而下调。

项目成果

期刊论文数量(11)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Derek Bartlem: "Mutation in the threonine synthase gene results in an over-accumulation of soluble methionine in Arabidopsis."Plant Physiology. 123・5. 101-110 (2000)
Derek Bartlem:“苏氨酸合酶基因的突变导致拟南芥中可溶性蛋氨酸的过度积累。”植物生理学 123・5 (2000)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yukako Chiba: "Evidence for autoregulation of cystathionine g-synthase mRNA stability in Arabidopsis"Science. 286-5443. 1371-1374 (1999)
Yukako Chiba:“拟南芥中胱硫醚 g-合酶 mRNA 稳定性自动调节的证据”《科学》。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yukako Chiba: "Evidence for autoregulation of cystathionine γ-synthase mRNA stability in Arabidopsis."Science. 286・5443. 1371-1374 (1999)
Yukako Chiba:“拟南芥中胱硫醚 γ-合酶 mRNA 稳定性的自动调节证据。”《科学》286·5443(1999)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Derek Bartlem: "Mutation in the threonine synthase gene results in an overaccumulation of soluble methionine in Arabidopsis."Plant Physiology. 123(5). 101-110 (2000)
德里克·巴特勒姆:“苏氨酸合酶基因突变导致拟南芥中可溶性蛋氨酸过度积累。”植物生理学。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Derek Bartlem: "Genomic nucleotide sequence of the Arabidopsis threonine synthase gene (Accession No.AB027151)."Plant Physiology. 120 (4). 1205-1205 (1999)
Derek Bartlem:“拟南芥苏氨酸合酶基因的基因组核苷酸序列(登录号AB027151)。”植物生理学。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
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NAITO Satoshi其他文献

NAITO Satoshi的其他文献

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{{ truncateString('NAITO Satoshi', 18)}}的其他基金

Starting up the functional ribosome-omics (ribosomomics): an approach from the translation arrest
启动功能性核糖体组学(核糖体组学):一种来自翻译停滞的方法
  • 批准号:
    16K14746
  • 财政年份:
    2016
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Realization of the crystal bases of level-zero representations of quantum affine algebras as algebraic cycles
量子仿射代数零级表示的晶体基作为代数循环的实现
  • 批准号:
    20540006
  • 财政年份:
    2009
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular Mechanism of Nascent Peptide-Mediated Translation Arrest Involved in Feedback Regulation of Methionine Biosynthesis
新生肽介导的翻译阻滞参与蛋氨酸生物合成反馈调节的分子机制
  • 批准号:
    20370016
  • 财政年份:
    2008
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Regulation of RNA degradation by RNase
RNase 调节 RNA 降解
  • 批准号:
    17026001
  • 财政年份:
    2005
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Study on the fusion products and crystalbases of level-zero representations of quantum affine algebras
量子仿射代数零级表示的融合积和晶基研究
  • 批准号:
    17540008
  • 财政年份:
    2005
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular mechanism of translation arrest and mRNA degradation in cystathionine gamma-synthase, the key-step enzyme of methionine biosynthesis.
胱硫醚γ-合酶(甲硫氨酸生物合成的关键步骤酶)翻译停滞和 mRNA 降解的分子机制。
  • 批准号:
    16370016
  • 财政年份:
    2004
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Study on the path models for extremal weight modules over a quantum affine algebra
量子仿射代数极值权模路径模型研究
  • 批准号:
    14540006
  • 财政年份:
    2002
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular genetic studies of control of mRNA stability in the gene for the key enzyme of methionine biosynthesis
蛋氨酸生物合成关键酶基因mRNA稳定性控制的分子遗传学研究
  • 批准号:
    13440233
  • 财政年份:
    2001
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Mechanism of response of seed storage protein gene expression to nutritional signals
种子贮藏蛋白基因表达对营养信号的响应机制
  • 批准号:
    12138201
  • 财政年份:
    2000
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Molecular Biological Studies on the Regulation of MeEthionine Biosynthesis Using an Arabidopsis thaliana Mutant.
使用拟南芥突变体调节甲硫氨酸生物合成的分子生物学研究。
  • 批准号:
    09440262
  • 财政年份:
    1997
  • 资助金额:
    $ 10.69万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

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构建拟南芥形态学模板模型用于计算突变体筛选
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