Studies on the origin of apogamy and speciation of Cornopteris

角蕨属无配生殖起源及物种形成研究

• 批准号: 11440245
• 负责人: KATO Masahiro
• 金额: $ 9.41万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11440245/
• 关键词: Apogamy; Chromosome number; Cornopteris christenseniana; Enzyme electrophoretic analysis; Evolution; Gametophyte; Nucleotide sequence analysis; Sporogenesis; ハコネシケチシダ; 非滅数; 酵素多型; DNA塩基配列; 種内構造; 多起源; 韓国; 3倍体; 胞子; 種間雑種; 種分化

This study on the origin of apogamy in plants obtained the following results :1. Cornopteris christenseniana propagated in an artificial environment (Fern Garden) in the University of Tokyo Botanical Gardens, although it is considered to be a natural triploid sterile fern derived by hybridization between C. crenulatoserrulata and C. decurrenti-alata. It indicates that C. christenseniana is able to reproduce in such a condition.2. An electrophoretic analysis of allozyme variations and a molecular phylogenetic analysis of chloroplast DNA showed that most of young plants propagated by apogamy from mature plants of C. christenseniana grown in or near the Fern Garden. By contrast, hybridization between the parental species is not involved in propagation of C. christenseniana in the Fern Garden. Furthermore, the results showed that some plants of C. christenseniana, which were transplantedサfrom various localities, were derived by hybridization involving C. crenulatoserrulata as mother and the others, involving C. decurrenti-alata as mother, indicating multiple origins of C. christenseniana.3. Observations of sporogenesis indicated that C. christenseniana produces a few viable unreduced spores in a different sporogenetic pathway from that of Dopp-Manton and Braithwaite apogamy systems.4. C. christenseniana is considered to be a species complex consisting of populations which are various in the frequencies of viable spore and gametophyte production, ranging from a high (about 80 %) to a low (about 1 %) frequency.5. Our results support the hypothesis that different mutations are involved independently in the origin of unreduced sporogenesis and apogamy in the gametophyte generation (sensu stricto), as compared with the single-gene hypothesis.

本研究对植物无配子生殖的起源进行了探讨，取得了以下结果：1。虽然被认为是天然的三倍体不育蕨类植物，但在东京大学植物园的人工环境（蕨类植物园）中繁殖的Cornopteris christenseniana，是通过C. crenulatoserrulata和C.下弯翼形表明C. christenseniana能够在这样的条件下繁殖。等位酶电泳分析和叶绿体DNA分子系统发育分析表明，绝大多数幼株是由成熟植株通过无配子生殖方式繁殖而来的。在蕨类植物园或附近生长的christenseniana。与此相反，亲本种之间的杂交不参与C。在蕨类植物园的圣诞树。此外，研究结果还表明，部分C. christenseniana是从不同地方移植过来的，它们是通过与C. crenulatoserrulata为母本，其余为C. decurrenti-alata为母本，表明C. christenseniana.3.孢子发生观察表明，C. christenseniana产生少量有活力的未减数孢子的途径与Dopp-Manton和Braithwaite无配子生殖系统不同. C. christenseniana被认为是一个由具有不同的可存活孢子和配子体产生频率的种群组成的物种复合体，其频率范围从高（约80%）到低（约1%）。我们的研究结果支持这一假设，即不同的突变独立参与的起源未减少孢子发生和无配子生殖的配子体一代（狭义），与单基因假说相比。

项目成果

Kato, M.: "Deparia cataracticola (Woodsiaceae), a new Species from Hawaii"Acta Phytotaxonomica et Geobotanica. 52(1). 1-9 (2001)

Kato, M.：“Deparia cataracticola（Woodsiaceae），夏威夷新种”Acta Phytotaxonomica et Geobotanica。

Kato, M.: "An rbcL-based phylogeny and heteroblastic leaf morphology of Matoniaceae"Syst. Bot.. 23. 391-400 (1999)

Kato, M.：“基于 rbcL 的 Matoniaceae 系统发育和异胚叶形态”Syst。

Kato, M.: "Deparia cataracticola (Woodsiaceae) , a new species from Hawaii"Acta Phytotax. Geobot.. 52. 1-9 (2001)

Kato, M.：“Deparia cataracticola（Woodsiaceae），来自夏威夷的新物种”Acta Phytotax。

Kato, M.: "A Cytotaxonomic study of Hainan (S. China) ptenmophytes with notes on polyplidy and apogamy of chinese ferns"Ching Mem. Vol.. 1-19 (1999)

Kato, M.：“海南（中国南部）蕨类植物的细胞分类学研究以及中国蕨类植物的多倍性和无性繁殖的注释”Ching Mem。

Kato, M.: "A cytotaxonomic study of Hainan (S. China) pterdophytes with notes on polyploidy and apogamy of Chinese ferns"Ching Mem. Vol.,1-19. (1999)

Kato, M.：“海南（中国南部）蕨类植物的细胞分类学研究以及中国蕨类植物的多倍体和无性繁殖的注释”Ching Mem。