Studies on the origin of angiosperms and the evolution of ovules

被子植物起源和胚珠进化的研究

• 批准号: 15370035
• 负责人: KATO Masahiro
• 金额: $ 9.47万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15370035/
• 关键词: angiosperm; ovule; carpel; outer integument; dorsiventrality; YABBY genes; INNER NO OUTER; evolution; INNER NO OUTER遺伝子; スイレン科; アンボレラ; 原始的被子植物

1.A morphological analysis of ovules of Magnolia grandiflora, Magnoliaceae, was performed in order to understand the morphology of the primitive ovule of angiosperms. The outer integument arose as a hood-shaped structure and then became cupular with an outgrowth arising on the funiculus between a gap in the integument. In the ovules of three families assigned to the primitive order Austrobaileyales, the outer integument is hood-shaed in Austrobaileya and Ttimernia, while it is copular in Illicium. Taking in account data of other primitive angiosperms and phylogenetic relationships of angiosperms, it is inferred that the primitive ovule is the anatropous one with the hood-shaped outer integument, which is similar to the lateral organ leaf.2.In order to better understand the homology of the ovule and that of the integument of primitive angiosperms at gene level, expression patterns were analyzed for homologues of genes of the YABBY family, which are involved in determination of the dorsiventrality of lateral organs. NaINO, an INO homologue, isolated from Nymphaea, Nymphaeaceae, was expressed in the outer layer of the outer integument. The result suggested that the outer integument may be a lateral organ homologous to the leaf. AmbF1, a YABBY2 homologue, isolated from Amborella, was expressed in the dorsal tissue of the leaf and the inner tissue of the carpet, patterns distinct from those of the advanced eudicot Arabidopsis thaliana, a model plant. These results are not in accordance with the widely accepted hypotheses that the carpel is a con duplicate leaf or an ascidiate structure derived from a peltate leaf.3.In conclusion, it is inferred the outer integument unique to angiosperms arose from a lateral organ such as the leaf and then diversified, and NaINO and AmbF1 homologous to YABBY family genes that govern the specificity of lateral organs evolved and their expression patterns changed in association with the origin and diversification of angiosperms.

1.对木兰科植物广花木兰（Magnolia grandiflora）的胚珠进行了形态学分析，以了解被子植物原始胚珠的形态。外珠被出现作为一个帽状结构，然后成为杯状与珠柄之间的差距在珠被上产生的产物。在原始Austrobaileyales目的三个科的胚珠中，Austrobaileya和Ttimernia的外珠被为帽状，而Illicium的外珠被为系珠。结合其他原始被子植物的资料和被子植物的系统发育关系，推测原始胚珠为倒生胚珠，具有类似侧生器官叶的帽状外珠被。2.为了更好地了解原始被子植物胚珠与珠被在基因水平上的同源性，对YABBY家族基因的同源表达模式进行了分析，其参与确定侧部器官的背腹性。NaINO是从睡莲科睡莲属植物中分离的INO同源物，在外珠被的外层表达。结果表明，外珠被可能是与叶同源的侧生器官。AmbF1是从Amborella中分离的YABBY2同源物，在叶背组织和地毯的内部组织中表达，其模式与模式植物拟南芥（Arabidopsis thaliana）不同。这些结果不符合普遍接受的心皮是由盾形叶演化而来的一个连生叶或一个鞘状结构的假说。3.综上所述，被子植物特有的外珠被起源于一个侧生器官，如叶，然后分化，与控制侧器官特异性的YABBY家族基因同源的NaINO和AmbF1进化，其表达模式随着起源而改变和被子植物的多样化。

项目成果

Yamada, T., M.Ito, M.Kato: "Expression pattern of INNER NO OUTER homologue in Nymphaea (water lily family, Nymphaeaceae)."Dev.Genes Evol.. 213. 510-513 (2003)

Yamada, T., M.Ito, M.Kato：“Nymphaea（睡莲科，睡莲科）中 INNER NO OUTER 同源物的表达模式。”Dev.Genes Evol.. 213. 510-513 (2003)

Yamada, T., R.Imaichi, M.Kato: "The outer integument and funicular outgrowth complex in the ovule of Magnolia grandiflora (Magnoliaceae)."J.Plant Res.. 116. 189-198 (2003)

Yamada, T., R.Imaichi, M.Kato：“广玉兰（木兰科）胚珠中的外珠被和索状生长复合体。”J.Plant Res.. 116. 189-198 (2003)

YABBY2-homologue expression in lateral organs of Amborella trichopoda (Amborellaceae)

YABBY2-同源物在毛足安博氏菌（Amborellaceae）侧器官中的表达

• 发表时间: 2004
• 期刊: Int.J.Plant Sci. 165
• 作者: Yamada;T.
• 通讯作者: T.

Yamada, T., R.Imaichi, N.Prakash, M.Kato: "Developmental morphology of ovules and seeds of Austrobaileyales."Aust.J.Bot.. 51. 555-565 (2003)

Yamada, T., R.Imaichi, N.Prakash, M.Kato：“Austrobaileyales 胚珠和种子的发育形态。”Aust.J.Bot.. 51. 555-565 (2003)