权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Effect of emotional state induced by expectation for pain on cortical neuronal activity in awake behaving monkeys

疼痛预期诱发的情绪状态对清醒行为猴皮质神经元活动的影响

基本信息

批准号：
11470390
负责人：
IWATA Koichi
金额：
$ 9.79万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

In order to clarify the effect of emotional state induced by expectation for pain on cortical neuronal activity, single neuronal activities were recorded form the Primary somatosensory cortex (SI) or Anterior cingulated cortex (ACCx) and the correlation between behavior and neuronal responses were analyzed Monkeys were sitting quietly on the monkey chair. When the "Go light" conies on, monkeys press the button. Then the heat stimulus is applied to the facial skin through the thermal probe. We introduced three different types of detection trials for monkey's perceived intensity of heat stimulus intensity. 1) Heat detection: T1 temperature consists with 44-46℃ and T2 temperature is 0.2-0.8℃. Monkey need to try to detect the temperature change using a heat stimulus without light. 2) Light detection: When the monkey presses the button, L1 (basic light) comes on. After 4 to 8 second hold period, L2 (a small light intensity increment) is added on L1. Monkeys detect the change in L2 change and get reward. 3) Light detection with heat: When the monkeys press the button, L1 light comes on. With 44-46℃ heat. After 4- 8 second hold time, L2 comes on. Monkey need to detect the change in light intensity change. Many nociceptive neurons in SI and ACCx increased firing frequency following increase in stimulus temperature. When monkeys escaped from T1 before T2 coming, nociceptive neurons significantly increased their firing frequency in ACCx. The firing frequency of the S1 nociceptive neurons significantly correlated with detection latency, while ACCx neurons did not show any correlation.These suggest that SI neurons would be involved in sensory discriminative aspect of pain whereas, ACCx nociceptive neurons would be involved in motivational aspect of pain, and also nociceptive neuron sin both areas would be involved in attention of pain.

为了阐明疼痛预期引起的情绪状态对皮层神经元活动的影响，记录了初级躯体感觉皮层（SI）或前扣带皮层（ACCx）的单个神经元活动，并分析了行为与神经元反应之间的相关性。当“启动灯”亮起时，猴子按下按钮。然后通过热探头将热刺激施加到面部皮肤。介绍了三种不同类型的猴热刺激强度感知强度检测试验。1)热检测：T1温度为44-46℃，T2温度为0.2-0.8℃。猴子需要尝试使用没有光的热刺激来检测温度变化。2)光检测：当猴子按下按钮时，L1（基本光）亮起。经过4到8秒的保持时间后，L2（一个小的光强增量）被添加到L1上。猴子检测到L2变化并获得奖励。3)光检测伴热：猴按下按钮，L1灯亮，伴44-46℃热。保持4- 8秒后，L2开始。猴子需要检测光强度的变化。SI和ACCx中的许多伤害性神经元在刺激温度升高后放电频率增加。当猴在T2到来之前逃离T1时，ACCx伤害性感受神经元的放电频率显著增加。S1区伤害性感受神经元的放电频率与检测潜伏期显著相关，而ACCx区伤害性感受神经元的放电频率与检测潜伏期无相关性，提示S1区伤害性感受神经元参与疼痛的感觉辨别功能，ACCx区伤害性感受神经元参与疼痛的动机功能，两个区的伤害性感受神经元均参与疼痛的注意功能。