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The role of c-Met oncogene expression in the secondary palate shelves adhesion process.

c-Met癌基因表达在次级腭架粘附过程中的作用。

基本信息

批准号：
11470397
负责人：
YOSHIDA Hideo
金额：
$ 6.66万
依托单位：
Tokushima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

BALB/C mouse was used in the identification of the localization of HGF, c-Met, etc. in the mouse foetus secondary palate shelves. The foetus is exposed from the dam from pregnancy 14th to 15th in 6 hour by anesthetize, and the paraffin section is produced, and HGF, c-Met oncogene protein, the cytokeratin which is the epithelial cell marker, vimentin which is the mesenchymal cell marker were examined by immunostaining procedure. As the result, there was the expression in which c-Met was mainly extensive for whole palate shelves before the adhesion 14th for HGF in the epitherial layer of palate shelves, and the vimentin observed the mesenchymal tissue, and the cytokeratin was recognized only in the epitherial layer. The adhesion took the horizontal positioning on secondary palate shelves near, and c-Met expression was strengthened. The expression of HGF is abated a little right after the adhesion in the epitherial layer, and it increase mesenchyme layer a little. The expression of c-Met … More reversely decreased in the mesenchymal organization a little, and it was strengthened in the epitherial layer. There was some the resistant expression on the vimentin with the adhesion division epithelium in the circumference. And, there was the coloration on the cytokeratin in the epitherial layer. HGF, c-Met was abated in the adhesion completion in the whole. Vimentin there palate whole area expression, so that the cytokeratin may diffuse from the adhesion division, there mesenchymal extensiv, that is to say, epithelium marker cytokeratin mesenchymal simultaneously, there identical expression, it was indicated that HGF, c-Met induced the conversion of epithelium-mesenchymal tissue. It was indicated that the apoptosis was concerned in that it extensively appears palate shelves 14th and that the protrusion moves from the vertical position to horizontal positioning on Nick-End labelling which is an index to the apoptosis. In addition, HGF gene expression was confirmed in DNA-RNA in situ hybridization. Less

采用BALB/C小鼠，对肝细胞生长因子（HGF）、c-Met等在小鼠胚胎次级腭突中的定位进行了鉴定。将孕14 ~ 15天的胎儿在麻醉状态下6 h内从母鼠体内取出，制作石蜡切片，免疫组化法检测HGF、c-Met癌基因蛋白、上皮细胞标志物细胞角蛋白、间质细胞标志物波形蛋白。结果表明，HGF在14天粘附前，c-Met主要在整个腭架上广泛表达，波形蛋白主要在间充质组织中表达，而细胞角蛋白仅在腭架上皮层中表达。粘连呈水平定位于次级腭突附近，c-Met表达增强。粘附后上皮层HGF表达稍有减弱，间质层HGF表达稍有增加。c-Met的表达 ...更多信息间充质组织中的胶原蛋白含量略有减少，而上皮层中的胶原蛋白含量有所增加。周围粘连分裂上皮的波形蛋白有一定的抵抗性表达。表皮层细胞角蛋白染色。HGF、c-Met在粘附完成中的作用总体减弱。Vimentin在腭部全区域表达，使细胞角蛋白可从粘连处扩散，在间充质处广泛表达，即上皮标志细胞角蛋白间充质同时表达，表明HGF、c-Met诱导了上皮-间充质组织的转化。结果表明，第14腭突广泛出现与细胞凋亡有关，Nick-End标记上突起由垂直位置向水平位置移动是细胞凋亡的指标。此外，肝细胞生长因子基因表达的DNA-RNA原位杂交证实。少