The role of c-Met oncogene expression in the secondary palate shelves adhesion process.

c-Met癌基因表达在次级腭架粘附过程中的作用。

基本信息

  • 批准号:
    11470397
  • 负责人:
  • 金额:
    $ 6.66万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
  • 财政年份:
    1999
  • 资助国家:
    日本
  • 起止时间:
    1999 至 2000
  • 项目状态:
    已结题

项目摘要

BALB/C mouse was used in the identification of the localization of HGF, c-Met, etc. in the mouse foetus secondary palate shelves. The foetus is exposed from the dam from pregnancy 14th to 15th in 6 hour by anesthetize, and the paraffin section is produced, and HGF, c-Met oncogene protein, the cytokeratin which is the epithelial cell marker, vimentin which is the mesenchymal cell marker were examined by immunostaining procedure. As the result, there was the expression in which c-Met was mainly extensive for whole palate shelves before the adhesion 14th for HGF in the epitherial layer of palate shelves, and the vimentin observed the mesenchymal tissue, and the cytokeratin was recognized only in the epitherial layer. The adhesion took the horizontal positioning on secondary palate shelves near, and c-Met expression was strengthened. The expression of HGF is abated a little right after the adhesion in the epitherial layer, and it increase mesenchyme layer a little. The expression of c-Met … More reversely decreased in the mesenchymal organization a little, and it was strengthened in the epitherial layer. There was some the resistant expression on the vimentin with the adhesion division epithelium in the circumference. And, there was the coloration on the cytokeratin in the epitherial layer. HGF, c-Met was abated in the adhesion completion in the whole. Vimentin there palate whole area expression, so that the cytokeratin may diffuse from the adhesion division, there mesenchymal extensiv, that is to say, epithelium marker cytokeratin mesenchymal simultaneously, there identical expression, it was indicated that HGF, c-Met induced the conversion of epithelium-mesenchymal tissue. It was indicated that the apoptosis was concerned in that it extensively appears palate shelves 14th and that the protrusion moves from the vertical position to horizontal positioning on Nick-End labelling which is an index to the apoptosis. In addition, HGF gene expression was confirmed in DNA-RNA in situ hybridization. Less
采用BALB/C小鼠,对肝细胞生长因子(HGF)、c-Met等在小鼠胚胎次级腭突中的定位进行了鉴定。将孕14~15天的胎儿在麻醉状态下6 h内从母鼠体内取出,制作石蜡切片,免疫组化法检测HGF、c-Met癌基因蛋白、上皮细胞标志物细胞角蛋白、间质细胞标志物波形蛋白。结果表明,HGF在14天粘附前,c-Met主要在整个腭架上广泛表达,波形蛋白主要在间充质组织中表达,而细胞角蛋白仅在腭架上皮层中表达。粘连呈水平定位于次级腭突附近,c-Met表达增强。粘附后上皮层HGF表达稍有减弱,间质层HGF表达稍有增加。c-met的表达 ...更多信息 间充质组织中的胶原蛋白含量略有减少,而上皮层中的胶原蛋白含量有所增加。周围粘连分裂上皮的波形蛋白有一定的抵抗性表达。表皮层细胞角蛋白染色。HGF、c-Met在粘附完成中的作用总体减弱。Vimentin在腭部全区域表达,使细胞角蛋白可从粘连处扩散,在间充质处广泛表达,即上皮标志细胞角蛋白间充质同时表达,表明HGF、c-Met诱导了上皮-间充质组织的转化。结果表明,第14腭突广泛出现与细胞凋亡有关,Nick-End标记上突起由垂直位置向水平位置移动是细胞凋亡的指标。此外,肝细胞生长因子基因表达的DNA-RNA原位杂交证实。少

项目成果

期刊论文数量(8)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kawamata H.: "Haematogenous cytokeratin 20 mRNA as a predictive marker for recurrence in oral cancer patients."British Journal of Cancer.. Vol80(3). 448-452 (1999)
Kawamata H.:“血源性细胞角蛋白 20 mRNA 作为口腔癌患者复发的预测标记。”英国癌症杂志第 80 卷(3)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
小林章祐: "マウス胎仔二次口蓋突起癒合過程におけるHGF/SFとc-Metの役割"日本口蓋裂学会雑誌. 24巻2号. 255 (1999)
Shosuke Kobayashi:“HGF/SF 和 c-Met 在小鼠胎儿次级腭突融合过程中的作用”日本裂腭协会杂志,第 24 卷,第 2. 255 期(1999 年)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Kobayasi S, Yoshida H, Yura Y and Sato M: "Role of HGF/SF and c-Met in the secondary palatal sheves adhesion process."Nihon kogairetugakkaizassi (japanese). vol 24, No.2. 255 (1999)
Kobayasi S、Yoshida H、Yura Y 和 Sato M:“HGF/SF 和 c-Met 在次级腭滑粘连过程中的作用。”Nihon kogairetugakkaizassi(日语)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Kawamata H, Uchida D, Nakashiro K, Hino S, Omotehara F, Yoshida H and Sato M: "Haematogenous cytokeratin 20 mRNA as a predictive marker for recurrence in oral cancer patients."British Journal of Cancer. Vol 80, No.3. 448-452 (1999)
Kawamata H、Uchida D、Nakashiro K、Hino S、Omotehara F、Yoshida H 和 Sato M:“血源性细胞角蛋白 20 mRNA 作为口腔癌患者复发的预测标记。”英国癌症杂志。
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  • 期刊:
  • 影响因子:
    0
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YOSHIDA Hideo其他文献

YOSHIDA Hideo的其他文献

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{{ truncateString('YOSHIDA Hideo', 18)}}的其他基金

Establishment of a novel therapeutic approach targeting cancer stem cells of neuroblastoma using onclytic virus
利用溶瘤病毒建立针对神经母细胞瘤癌症干细胞的新型治疗方法
  • 批准号:
    22591976
  • 财政年份:
    2010
  • 资助金额:
    $ 6.66万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Control and Enhancement of Successive Reaction of Steam Reforming of Dimethyl Ether in a Pressure Gradient in Micro Nozzle
微喷嘴压力梯度下二甲醚水蒸气重整连续反应的控制与强化
  • 批准号:
    21360096
  • 财政年份:
    2009
  • 资助金额:
    $ 6.66万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
The effect of professional oral health care on improving autoimmune disease
专业口腔保健对改善自身免疫性疾病的作用
  • 批准号:
    20390536
  • 财政年份:
    2008
  • 资助金额:
    $ 6.66万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Immune system of the intestinal mucosa and effects of probiotics in inflammatorybowel disease
肠粘膜免疫系统及益生菌在炎症性肠病中的作用
  • 批准号:
    18591950
  • 财政年份:
    2006
  • 资助金额:
    $ 6.66万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of the therapy with an intratumoral administration of dendritic cells in combination with radiation or anti-cancer drugs against oral cancer : Application to clinical trial.
树突状细胞瘤内给药联合放射或抗癌药物治疗口腔癌的疗法的开发:应用于临床试验。
  • 批准号:
    16592006
  • 财政年份:
    2004
  • 资助金额:
    $ 6.66万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of Hybrid Gas Bearing Effectively Stabilized by Water Evaporation from Ultra-Fine Porous Medium
超细多孔介质蒸发水有效稳定混合气体轴承的研制
  • 批准号:
    15360108
  • 财政年份:
    2003
  • 资助金额:
    $ 6.66万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
The correlation between TGF-b3 and HGF/SF in the process of fusion of secondary palatal shelves
次级腭架融合过程中TGF-b3与HGF/SF的相关性
  • 批准号:
    13671968
  • 财政年份:
    2001
  • 资助金额:
    $ 6.66万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Mixing Control System Using Micro Nozzle of 3D Vortex Generator and Flame holder Aiming at High-Speed Diffusion Combustion
利用3D涡流发生器和火焰保持器的微喷嘴实现高速扩散燃烧的混合控制系统
  • 批准号:
    12450087
  • 财政年份:
    2000
  • 资助金额:
    $ 6.66万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
GENE THERAPY FOR NEUROBLASTOMA
神经母细胞瘤的基因治疗
  • 批准号:
    12671735
  • 财政年份:
    2000
  • 资助金额:
    $ 6.66万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of Microactuators Using Temperature Dependence of Surface Tension
利用表面张力的温度依赖性开发微执行器
  • 批准号:
    11555058
  • 财政年份:
    1999
  • 资助金额:
    $ 6.66万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

相似海外基金

再生肝と肝発癌過程における,c-met oncogeneの発現
c-met癌基因在再生肝及肝癌发生中的表达
  • 批准号:
    04770815
  • 财政年份:
    1992
  • 资助金额:
    $ 6.66万
  • 项目类别:
    Grant-in-Aid for Encouragement of Young Scientists (A)
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