The role of c-Met oncogene expression in the secondary palate shelves adhesion process.

c-Met癌基因表达在次级腭架粘附过程中的作用。

基本信息

批准号：
11470397
负责人：
YOSHIDA Hideo
金额：
$ 6.66万
依托单位：
Tokushima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

BALB/C mouse was used in the identification of the localization of HGF, c-Met, etc. in the mouse foetus secondary palate shelves. The foetus is exposed from the dam from pregnancy 14th to 15th in 6 hour by anesthetize, and the paraffin section is produced, and HGF, c-Met oncogene protein, the cytokeratin which is the epithelial cell marker, vimentin which is the mesenchymal cell marker were examined by immunostaining procedure. As the result, there was the expression in which c-Met was mainly extensive for whole palate shelves before the adhesion 14th for HGF in the epitherial layer of palate shelves, and the vimentin observed the mesenchymal tissue, and the cytokeratin was recognized only in the epitherial layer. The adhesion took the horizontal positioning on secondary palate shelves near, and c-Met expression was strengthened. The expression of HGF is abated a little right after the adhesion in the epitherial layer, and it increase mesenchyme layer a little. The expression of c-Met … More reversely decreased in the mesenchymal organization a little, and it was strengthened in the epitherial layer. There was some the resistant expression on the vimentin with the adhesion division epithelium in the circumference. And, there was the coloration on the cytokeratin in the epitherial layer. HGF, c-Met was abated in the adhesion completion in the whole. Vimentin there palate whole area expression, so that the cytokeratin may diffuse from the adhesion division, there mesenchymal extensiv, that is to say, epithelium marker cytokeratin mesenchymal simultaneously, there identical expression, it was indicated that HGF, c-Met induced the conversion of epithelium-mesenchymal tissue. It was indicated that the apoptosis was concerned in that it extensively appears palate shelves 14th and that the protrusion moves from the vertical position to horizontal positioning on Nick-End labelling which is an index to the apoptosis. In addition, HGF gene expression was confirmed in DNA-RNA in situ hybridization. Less

BALB/C小鼠用于鉴定HGF，C-MET等的定位。胎儿在6小时内通过麻醉从妊娠14号到15位暴露于大坝，并产生石蜡段，HGF，C-MET癌基因蛋白（细胞增生蛋白）是上皮细胞标记物，是上皮细胞标记物，vimentin，vimentin，是间质细胞标记，通过免疫抑制过程检查了中质细胞标记。结果，在粘合剂的粘膜14th胶架上的粘合剂第14位，C-MET的表达主要是广泛的。粘合剂将水平定位在附近的次级味架上，并加强了C-MET表达。在粘着层中的粘合剂后，HGF的表达逐渐消失，并稍微增加了间质层。 C-MET的表达…在间充质组织中略有下降，并且在层状层中得到了加强。波形蛋白上有一些抗性表达，周长的粘合剂分裂。而且，在层状层中的细胞角蛋白上有颜色。 HGF，C-MET在整体上的粘合剂完成中均匀。那里的波形蛋白在那里的整个区域表达上可口，因此细胞角蛋白可能会从粘附分裂中扩散出间充质的扩展IV，也就是说，上皮标记物细胞角蛋白间充质的间充质很简单，因此表达相同，这表明HGF，C-MET诱导了上皮细胞膜组织的转化。据表明，凋亡涉及凋亡是因为它广泛地出现了味觉架子第14位，并且突出从垂直位置移动到划痕标记上的水平位置，这是凋亡的指数。此外，在原位杂交中证实了HGF基因表达。较少的