Effect of site selective cAMP & its metabolite on human adenocarucinoma cell line

位点选择性 cAMP 的作用

• 批准号: 07672177
• 负责人: YOSHIDA Hideo
• 金额: $ 1.41万
• 依托单位: University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672177/
• 关键词: 8-chloro-aenosine 3' : 5'-monophosphate (8-Cl-cAMP); 8-chloro-adensine; human adenocarcinoma cell line; cAMP-dependent protein kinase (PKA); cell proliferation

We examined the effects of 8-chloro-adenosine 3' : 5'-monophosphate (8-Cl-cAMP), site-selective analogue of cAMP,and its metabolite 8-chloro-adenosine (8-Cl-ad) on the proliferation of human adenocarcinoma cell line HSG cells and its derivative cell line HSG-AZA1, HSG-AZA3, and human adenoid squamou carcinoma cell line TYS cells in culture. Cultivation of these cells in thepresence of 8-Cl-cAMP (0.5muM) or 8-Cl-ad (lmuM) resulted in the growth inhibition and reduction of colony formation rates on plastic surface or in soft agar to under 10%. Whereas the morphological alteration of treated cells were not shown. Then, it was confirmed that the HSG cells treated with 8-Cl-cAMP (0.5muM) or 8-Cl-ad (1muM) were not induced apoptosis, because DNA pattern was similar to untreated cells in agar gel electrophoresis. In addition, the cAMP-dependent protein kinase A,isolated from treated HSG cells, were decreased.

本实验观察了8-氯腺苷3'：5'-单磷酸（8-Cl-cAMP）及其代谢产物8-氯腺苷（8-Cl-ad）对体外培养的人腺癌细胞系HSG及其衍生细胞系HSG-AZA 1、HSG-AZA 3和人腺样鳞癌细胞系TYS增殖的影响。用8-Cl-cAMP（0.5 μ M）或8-Cl-ad（1 μ M）培养，细胞生长受到抑制，在塑料表面或软琼脂上的集落形成率降至10%以下。而处理细胞的形态学改变不明显。经8-Cl-cAMP（0.5 μ M）或8-Cl-ad（1 μ M）处理的HSG细胞，琼脂糖凝胶电泳DNA图谱与未处理的细胞相似，证实细胞未被诱导凋亡。此外，cAMP依赖性蛋白激酶A，从处理的HSG细胞分离，减少。

项目成果

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Sato M,Harada K,Bando T,Shirakami T,Nakashiro K,Yoshida H,Nakai S,Kawai K and Adachi M: "Characteristics of antitumor activity of 3,4-dihydro-6-[4- (3,4-dimethoxybenzoyl) -1-piperazinyl]-2 (1H)-quinolinone (vesnarinone) against a human adenoid squamous ca

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Sato M,Kawamata H,Harada K,Nakashiro K,Ikeda Y,Gohda H,Yoshida H,et al: "Induction of cyclin-dependent kinase inhibitor, p21^<KAF:> by treatment with 3,4-dihydro-6-[4-(3,4)-dimethoxybenzoyl)-1-piperazinyl]-2(1H)-quinolinone(vesnarinon) in a human salivary

Sato M、Kawamata H、Harada K、Nakashiro K、Ikeda Y、Gohda H、Yoshida H 等人：“通过 3,4-二氢-6- 处理诱导细胞周期蛋白依赖性激酶抑制剂，p21^<KAF:>

Sato M,Harada K,Bando T,Shirakami T,Nakashiro K,Yoshida H,et al: "Characteristics of antitumor activity of 3,4-dihydro-6-[4-(3,4-dimethoxy-benzoyl)-1-piperazinyl]-2(1H)-quinolinone(vesnarinone)against a human adenoid squamous carcinoma-forming cell line g

Sato M,Harada K,Bando T,Shirakami T,Nakashiro K,Yoshida H,et al：“3,4-二氢-6-[4-(3,4-二甲氧基-苯甲酰)-1-的抗肿瘤活性特征”