Adsorption behavior of protein, porphyrins and lipid onto the oil/water interface -Estimation with the laser evanescent wave at the interface.

蛋白质、卟啉和脂质在油/水界面上的吸附行为-用界面处的激光倏逝波进行估计。

• 批准号: 14340221
• 负责人: ISHIKAWA Yuichi
• 金额: $ 9.6万
• 依托单位: Oita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14340221/
• 关键词: evanescent wave; oil; water interface; estimation of molecular distribution; laser; protein adsorption; porphyrin adsorption; isoelectric point and adsorption; BSA protein; 混乱ポルフィリン; レーザーエバネッセント波; 界面活性剤; HLB; フッ素誘導体; キラル分割

Protein distribution around the oil-water interface was evaluated with evanescent wave emerged from the laser irradiation at the interface. The evanescent wave dipping into the aqueous phase allowed the molecule addressed at the region to ionize itself. The ionized molecules can be recognized as the change in electric current as long as some electric field is applied among the interface. Herein we tested to see if this method is applicable to adsorbed aqueous BSA proteins at the octane/water interface.Dependence of laser irradiation angle to the interface allows us to estimate the concentration of the ionized molecule 160 nm in depth from the interface. In the case of positive electric field (+500 V) from the octane oil phase, anionic BSAs being above the isoelectric point (4.7) located near the interface by 30-40 nm in depth. On the contrary, cationic BSAs being below the isoelectric point were away from the interface by 80-90 nm form the interface. In addition to the influence of the charges of the protein and of the applied electric field, the manner of the adsorption such as an allosteric and Langmuir adsorptions were observed. Several chiral N-confused porphyrin derivatives were prepared in this research.

利用激光在油水界面处产生的倏逝波来评价油水界面处蛋白质的分布。渐逝波浸入水相中，使该区域的分子能够自行分解。只要在界面间施加一定的电场，电离的分子就可以看作是电流的变化。在这里，我们测试，看看这种方法是否适用于吸附在辛烷/水interfaces.Dependence的激光照射角度的界面BSA水溶液的蛋白质，使我们能够估计离子化的分子的浓度从界面160 nm的深度。在来自辛烷油相的正电场（+500 V）的情况下，高于等电点（4.7）的阴离子BSA位于界面附近30-40 nm深。相反，低于等电点的阳离子BSA远离界面80-90 nm。除了蛋白质的电荷和所施加的电场的影响之外，还观察到吸附的方式，例如变构和Langmuir吸附。本研究合成了几种手性N-混乱卟啉衍生物。

项目成果

H.Maeda, Y.Ishikawa, H.Guruta: "N-confused Porphyrin-Bearing meso-Perfluorophenyl Goups : A Potential Agent That Forms Stable Sauare-Planar Comnlexes with Cu(II) and Ag(III)"Organic Letters. (in press). (2003)

H.Maeda、Y.Ishikawa、H.Guruta：“N-混淆的带有卟啉的内消旋全氟苯基基团：与 Cu(II) 和 Ag(III) 形成稳定的 Sauare 平面复合体的潜在试剂”有机字母。

M.Maeda, Y.Ishikawa, H.Furuta et al.: "N-Confused porphyrin-Bearing meso-Perfluoro phenyl Groups : A Potential Agent That Forms Stable Square-Planar Complexes with Cu(II) and Ag(III)"Organic Letters. 1293-1296 (2003)

M.Maeda、Y.Ishikawa、H.Furuta 等人：“N-Confused 卟啉-带有内消旋全氟苯基：与 Cu(II) 和 Ag(III) 形成稳定方形平面复合物的潜在试剂”有机

佐々木健夫, 石川雄一: "Photorefractive Effect of Liquid Crystalline Polymers Possessing Azobenzene Chromophores"Molecular Crystal and Liquid Crystal. 373. 53-70 (2002)

Takeo Sasaki、Yuichi Ishikawa：“具有偶氮苯发色团的液晶聚合物的光折变效应”《分子晶体与液晶》373. 53-70 (2002)。

佐々木健夫, 石川雄一ら: "Photorefractive Effect of Liquid Crystalline Polymers Possessing Azobenzene Chromophores."Molecular Crystal and Liquid Crystal. 373. 53-70 (2002)

Takeo Sasaki、Yuichi Ishikawa 等人：“具有偶氮苯发色团的液晶聚合物的光折变效应”。《分子晶体与液晶》373. 53-70 (2002)。

前田浩光, 石川雄一, 古田弘幸ら: "N-confused Porphyrin-Bearing meso-Perfluorophenyl Groups : A Potential Agent That Forms Stable Square-Planar Complexes with Cu(II) and Ag(III)."Organic Letters. 1293-1296 (2003)

Hiromitsu Maeda、Yuichi Ishikawa、Hiroyuki Furuta 等人：“N-混乱的带有内消旋全氟苯基的卟啉：与 Cu(II) 和 Ag(III) 形成稳定的方形平面复合物的潜在试剂。”有机快报 1293。 -1296 (2003)