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Analysis of molecular mechanism of long-lasting carnations

康乃馨长寿的分子机制分析

基本信息

批准号：
14360013
负责人：
SATOH Shigeru
金额：
$ 10.69万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14360013/
关键词：
long-lasting carnation fower senescence ehylene production petal wilting fading cysteine proteinase promoter 雌ずい遺伝子発現 ACC合成酵素 ACC酸化酵素

项目摘要

1.Analysis of ethylene production in the long-lasting carnation 'White Candle'In senescing carnation (Dianthus caryophyllus L.) flowers, ethylene is produced from the gynoecium, and acts as a diffusible signal received by petals to induce autocatalytic ethylene production in the petals. We discovered cv. ‘White Candle (WC)' as a long-lasting cultivar, of which cut flowers produce ethylene only in trace amounts and have a long vase life. The low ethylene production in these flowers was due to low ethylene production in the gynoecium, accompanied by low accumulation of ACC synthase (DC-ACS1) transcripts. These findings further support the importance of ethylene production from the gynoecium in the senescence of carnation flowers.2.Cloning and characterization of a cDNA for a novel carnation cysteine proteinaseThe wilting of carnation petals is caused by degradation of cell components, which leads to cell death during senescence of petals, mediated by some hydrolytic enzymes such as cysteine proteinase (CPase, DC-CP1), lipase and so on. We isolated a cDNA for a novel cysteine proteinase (DC-CP2) from carnation and compared its expression to that of DC-CP1 in senescing carnation petals. Furthermore, we isolated and characterized the nucleotide sequences of promoter region of both DC-CP1 and DC-CP2 genes.3.Analysis of wilting and fading in senescing carnation petalsWe categorized senescence of carnation flowers was into two types ; the first is 'wilting' which is induced by the action of ethylene and the second is 'fading' which occurs independent on ethylene. We revealed that a cysteine proteinase (DC-CP1) and its inhibitor (DC-CPIn) were up-regulated in carnation petals undergoing wilting as well as fading.

1.长寿康乃馨“白蜡烛”乙烯产生的分析在衰老的康乃馨(Dianthus caryophyllus L.)花中，乙烯由雌蕊产生，并作为花瓣接收的扩散信号，诱导花瓣中自催化乙烯的产生。我们发现了简历。 “白蜡烛（WC）”是一种寿命长的品种，其切花仅产生微量乙烯，并且具有较长的瓶插寿命。这些花中乙烯产量低是由于雌蕊中乙烯产量低，同时 ACC 合酶 (DC-ACS1) 转录物积累低。这些发现进一步支持了雌蕊产生乙烯在康乃馨花衰老过程中的重要性。2.新型康乃馨半胱氨酸蛋白酶cDNA的克隆和表征康乃馨花瓣的枯萎是由细胞成分降解引起的，这导致花瓣衰老过程中细胞死亡，这是由一些水解酶如半胱氨酸蛋白酶（CPase， DC-CP1)、脂肪酶等。我们从康乃馨中分离出了一种新型半胱氨酸蛋白酶 (DC-CP2) 的 cDNA，并将其表达与衰老康乃馨花瓣中 DC-CP1 的表达进行了比较。此外，我们还对DC-CP1和DC-CP2基因启动子区的核苷酸序列进行了分离和鉴定。3.康乃馨花瓣衰老过程中的萎蔫分析我们将康乃馨花朵的衰老分为两种类型：第一个是由乙烯作用引起的“萎蔫”，第二个是独立于乙烯而发生的“褪色”。我们发现，在枯萎和褪色的康乃馨花瓣中，半胱氨酸蛋白酶（DC-CP1）及其抑制剂（DC-CPIn）的表达上调。