Analysis of molecular mechanism of long-lasting carnations

康乃馨长寿的分子机制分析

基本信息

批准号：
14360013
负责人：
SATOH Shigeru
金额：
$ 10.69万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14360013/
关键词：
long-lasting carnation fower senescence ehylene production petal wilting fading cysteine proteinase promoter 雌ずい遺伝子発現 ACC合成酵素 ACC酸化酵素

项目摘要

1.Analysis of ethylene production in the long-lasting carnation 'White Candle'In senescing carnation (Dianthus caryophyllus L.) flowers, ethylene is produced from the gynoecium, and acts as a diffusible signal received by petals to induce autocatalytic ethylene production in the petals. We discovered cv. ‘White Candle (WC)' as a long-lasting cultivar, of which cut flowers produce ethylene only in trace amounts and have a long vase life. The low ethylene production in these flowers was due to low ethylene production in the gynoecium, accompanied by low accumulation of ACC synthase (DC-ACS1) transcripts. These findings further support the importance of ethylene production from the gynoecium in the senescence of carnation flowers.2.Cloning and characterization of a cDNA for a novel carnation cysteine proteinaseThe wilting of carnation petals is caused by degradation of cell components, which leads to cell death during senescence of petals, mediated by some hydrolytic enzymes such as cysteine proteinase (CPase, DC-CP1), lipase and so on. We isolated a cDNA for a novel cysteine proteinase (DC-CP2) from carnation and compared its expression to that of DC-CP1 in senescing carnation petals. Furthermore, we isolated and characterized the nucleotide sequences of promoter region of both DC-CP1 and DC-CP2 genes.3.Analysis of wilting and fading in senescing carnation petalsWe categorized senescence of carnation flowers was into two types ; the first is 'wilting' which is induced by the action of ethylene and the second is 'fading' which occurs independent on ethylene. We revealed that a cysteine proteinase (DC-CP1) and its inhibitor (DC-CPIn) were up-regulated in carnation petals undergoing wilting as well as fading.

1。分析持久康乃馨“白色蜡烛”感应康乃馨（Dianthus caryophyllus L.）花的花，乙烯是从妇科ecium产生的，是花瓣收到的可扩散信号，可作为Petals诱导乙烯产生的乙烯。我们发现了简历。 “白色蜡烛（WC）”是一种持久的品种，其中切花仅在痕量中产生乙烯，并具有较长的花瓶寿命。这些花中的低乙烯产生是由于妇科中的乙烯产量低，这是由于ACC合酶（DC-ACS1）转录本低积累而实现的。这些发现进一步支持了妇科元中乙烯产生在康乃馨花朵中的重要性。2。cDNA的克隆和表征对新型康纳化半胱氨酸蛋白质的新型cysateine蛋白质的萎缩是由细胞成分的降解引起的，导致细胞死亡在某些水解中，例如，在某些水解中（像底漆），像水性的杂物一样（像水解的杂物），这些细胞死亡（例如，水性含量），如水。 DC-CP1），脂肪酶等。我们从康乃馨分离了一种新型半胱氨酸蛋白酶（DC-CP2）的cDNA，并将其表达与传感康乃馨花瓣中的DC-CP1的表达进行了比较。此外，我们分离并表征了DC-CP1和DC-CP2基因的启动子区域的核苷酸序列。3。在传感康纳化petalswe中的枯萎和褪色的分析分类为康乃馨花的感觉分为两种类型。第一个是“枯萎”，它是由乙烯的作用引起的，第二个是“褪色”，它独立于乙烯。我们透露，半胱氨酸蛋白酶（DC-CP1）及其抑制剂（DC-CPIN）在康乃馨花瓣中被上调。