Preservation of cut carnation flowers by the use of chemicals without environmental toxicity

使用无环境毒性的化学品保存康乃馨切花

• 批准号: 07556009
• 负责人: SATOH Shigeru
• 金额: $ 2.82万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07556009/
• 关键词: ACBC; ACC oxidase; alpha-aminoisobutyrate; carnation; DPSS; ethylene; ethylene biosynthesis; flower preservation; アブシジン酸; フルリドン; スルホニルウレア剤; スクロース; AIB; 1-アミノシクロブタン-1-カルボン酸

(1) Effect of pre-harvest application of alpha-aminoisobutyrate (AIB) on the vase-life of cut carnation flowers.AIB,an analog of the ethylene precursor ACC,inhibits the the production of ethylene and lengthen the vase-life of cut carnation flowers. When AIB was administered to the roots of carnation plants before their flower opening, it was absorbed and transfered to flowers and prolonged the vase-life of the flowers.(2) Inhibition by ACBC of ACC oxidase obtained from senescing carnation petals.ACC oxidase was partially purified and characterized for its optimum pH (7-7.5), requirements for cofactors (Fe^<2+>, ascorbate, NaHCO_3), and Km for ACC (111-115 muM) and molecular mass (35kDa). ACBC (1-aminocyclobutane-1-carboxylate) competitively inhibited the action of the enzyme with Ki of 20-31 muM.ACBC and its N-substituted derivatives could lengthen the shelf-life of cut carnation flowers.(3) Mode of action of 1,1-dimethyl-4- (phenylsulfonyl) semicarbazide (DPSS)DPSS is a novel antisenescent presevative for cut carnation flowers. DPSS inhibits ethylene production from the flowers, resulting in lengthening thier vase-life. We revealed that DPSS did not inhibt the in vitro activities of ACC oxidase and ACC synthase obtained form senescing carnation petals. These findings indicated that DPSS has a unique mode of action, which is different from those of currently-known preservatives.

(1)采前施用α-氨基异丁酸（AIB）对香石竹切花瓶插寿命的影响AIB是乙烯前体ACC的类似物，它能抑制乙烯的产生，延长香石竹切花的瓶插寿命。在香石竹开花前将AIB施于香石竹根部，可使其吸收并转移到花中，延长花的瓶插寿命。(2)ACC氧化酶经部分纯化后，其最适pH值为7-7.5，对辅因子（Fe ~（2+）、抗坏血酸盐、NaHCO_3）的需求量为111-115 μ M，分子量为35 kDa。ACBC（1-aminocyclobutane-1-carboxylic acid）竞争性抑制该酶的活性，Ki值为20-31 μ M，ACBC及其N-取代衍生物能延长香石竹切花的货架期。(3)1，1-二甲基-4-（苯磺酰基）氨基脲（DPSS）的作用机理DPSS是一种新型的香石竹切花抗衰老保鲜剂。DPSS抑制乙烯从花中产生，从而延长其瓶插寿命。结果表明，DPSS对离体衰老香石竹花瓣ACC氧化酶和ACC合成酶的活性无明显影响。这些结果表明DPSS具有独特的作用模式，这与目前已知的防腐剂不同。

项目成果

Kosugi, Yusuke: "Inhibition by 1-aminocyclobutane-1-carboxylate of the activity of 1-aminocyclopropane-1-carboxylate oxidase obtained from senescing petals of carnation ( Dianthus caryophyllus L, ) flowers" Plant and Cell Physiology. 38 (3). (1997)

Kosugi，Yusuke：“1-氨基环丁烷-1-羧酸酯对从衰老的康乃馨花瓣中获得的 1-氨基环丙烷-1-羧酸酯氧化酶的活性的抑制”植物和细胞生理学。

Shigeru Satoh, Yusuke Kosugi, Naoko Oyamada, Toshihito Yoshioka, Eiichi Onodera and Yuko Yamada.: "Inhibition by 1-aminocyclobutane-1-carboxylate and its derivatives of 1-aminocyclopropane-1-carboxylate oxidase and their effects on the vase-life of cut ca

Shigeru Satoh、Yusuke Kosugi、Naoko Oyamada、Toshihito Yoshioka、Eiichi Onodera 和 Yuko Yamada.：“1-氨基环丁烷-1-羧酸酯及其衍生物对 1-氨基环丙烷-1-羧酸酯氧化酶的抑制及其对瓶插寿命的影响

Yusuke Kosugi, Naoko Oyamada, Shigeru Satoh, Toshihito Yoshioka, Eiichi Onodera and Yuko Yamada: "Inhibition by 1-aminocyclobutane-1-carboxylate of the activity of 1-aminocyclopropane-1-carboxylate oxidase obtained from senescing petals of carnation (Dian

Yusuke Kosugi、Naoko Oyamada、Shigeru Satoh、Toshihito Yoshioka、Eiichi Onodera 和 Yuko Yamada：“1-氨基环丁烷-1-羧酸酯对从康乃馨衰老花瓣中获得的 1-氨基环丙烷-1-羧酸酯氧化酶的活性的抑制（Dian）

小杉 祐介: "カ-ネーション花弁由来のACC酸化酵素の性質" 植物化学調節学会創立30周年記念大会研究発表記録集. 77-78 (1995)

Yusuke Kosugi：“来自康乃馨花瓣的 ACC 氧化酶的特性”植物化学监管协会 30 周年纪念会议上的研究报告记录 77-78 (1995)。

Yusuke Kosugi, Toshihito Yoshioka, Shigeru Satoh, Eiichi Onodera and Yuko Yamada.: "Characteritics of ACC oxidase obtained from senescing carnarion petals." Abstract for the 30th Aniversary Meeting of the Society for Chemical Regulation of Plants. 77-78 (

Yusuke Kosugi、Toshihito Yoshioka、Shigeru Satoh、Eiichi Onodera 和 Yuko Yamada.：“从衰老的石竹花瓣中获得的 ACC 氧化酶的特性。”