Assessment of genetic relatedness among cultivars of Pyrus pyrifolia and species native to east Asia by DNA analysis

通过 DNA 分析评估梨树品种与东亚原产物种之间的遗传相关性

• 批准号: 14360022
• 负责人: TANABE Kenji
• 金额: $ 9.54万
• 依托单位: National University Corporation Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14360022/
• 关键词: Sand pear; Ripening related genes; Rosaceae; Ethylene; O-methyltransferase; RAPD; 成熟関連遺伝子; Mite トランスポゾン; ナシ亜科; 果実成熟関連遺伝子; Malus

Genetic relationships of Pyrus pyrifolia and other Pyrus species native to east Asia were ambiguous. The origin of Japanese pear has two theories : 1.Development of Pyrus serotina native to Japan, 2.Imports from Pyrus pyrifolia (Sand pears) native to Central China. The theory for importing from China has an advantage, but the genetic relationship between Japanese pear and sand pear is unclear, therefore, the conclusion has not come yet. The research objective is to clarify the genetic relationships of Pyrus pyrifolia and other Pyrus species native to east Asia. We have cloned PPOMT1, similar t to O-methyltransferase, expressed in ripening fruits of Japanese pear and European pear, not in Chinese pear. We found this ripening gene was deleted in Chinese pear and that this is a useful marker for Pyrus classification. A few cultivars of sand pear have this gene and high homology to sequence of Japanese pear. This confirms the import theory. We classified ethylene synthetic genotypes of over 100 cultivars by ACC synthase genes (PPACS1 and PPACS2). This information is critical for making breeding strategies in Asian pears. Moreover, a total of 118 Pyrus spp.and cultivars native to east Asia were subjected to RAPD analysis. Some pear cultivars from Shikoku region were subclustered with some Chinese pears originated in Zhejiang province.

砂梨与东亚梨属其它种的亲缘关系不明确。日本梨的起源有两种说法：一种是日本梨的发展，另一种是中国中部砂梨的传入。从中国引进的理论具有一定的优势，但日本梨与砂梨的亲缘关系尚不清楚，尚未得出结论。本研究旨在阐明砂梨与东亚梨属其它种的亲缘关系。我们克隆了与O-甲基转移酶类似的PPOMT 1，它在日本梨和欧洲梨的成熟果实中表达，而在中国梨中没有表达。我们发现该成熟基因在中国梨中缺失，这是梨属分类的一个有用标记。砂梨的一些品种中含有该基因，且与日本梨的序列高度同源。这证实了进口理论。根据ACC合成酶基因（PPACS 1和PPACS 2）对100多个品种的乙烯合成基因型进行了分类。这些信息对于亚洲梨育种策略的制定至关重要。此外，共118梨种和品种原产于东亚进行了RAPD分析。四国地区的部分梨品种与原产于浙江的部分中国梨品种聚为一类。

项目成果

Development of DNA markers for ethylene production During fruit ripening in Japanese pear

日本梨果实成熟过程中乙烯产生的 DNA 标记的开发

• 发表时间: 2004
• 期刊: Hort.Res.(Japan) 3
• 作者: Itai;A.
• 通讯作者: A.

Genetic relationships of Pyrus species and cultivars native east Asia revealed by randomly amplified polymorphic DNA markers

随机扩增多态性 DNA 标记揭示东亚原产梨属物种和栽培品种的遗传关系

• 发表时间: 2002
• 期刊: J.Amer.Soc.Host.Sci. 127
• 作者: Teng;Y.;K.Tanabe;F.Tamura;A.Itai A.
• 通讯作者: A.Itai A.

ニホンナシ成熟果実のエチレン生成に関するDNAマーカーの開発

成熟日本梨果实乙烯生产 DNA 标记的开发

• 发表时间: 2004
• 期刊: 園芸学研究 3
• 作者: 板井章浩

The mechanism of ethylene production during fruit ripening in Asian pears.

亚洲梨果实成熟过程中乙烯产生的机制。

• 发表时间: 2002
• 期刊: Acta Hort. 587
• 作者: Itai;A.;K.Tanabe;F.Tamura
• 通讯作者: F.Tamura

Itai, A., T.Kotaki, K.Tanabe, F.Tamura, O.Kawaguchi, M.Fukuda: "Rapid identification of 1-aminocyclopropane-1-carboxylate (ACC) synthase genotypes in cultivars of Japanese pear (Pyrus pyrifolia Nakai) using CAPS markers"Theoretical and Applied Genetics. (

Itai, A.、T.Kotaki、K.Tanabe、F.Tamura、O.Kawaguchi、M.Fukuda：“快速鉴定日本梨 (Pyrus perifolia Nakai) 品种中 1-氨基环丙烷-1-羧酸酯 (ACC) 合酶基因型