Classification of Japanese Pear Cultivars by Characteristics of Ethylene Evolution in Ripening Fruits

根据成熟果实乙烯演化特征对日本梨品种进行分类

• 批准号: 03454049
• 负责人: TANABE Kenji
• 金额: $ 2.56万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454049/
• 关键词: Japanese pear; fruit ripening; ethylene forming enzyme; cultivar; polyamine; ethylene production; 1-aminocyclopropane-1-carboxylic acid; EFE活性; エチレン生成酵素(EFE); MACC; ポリアミン

The investigation on the relation between the ethylene production and storage characteristics of Jajpanese pears which connected with the ripening situation of frit was begun in 1989. It was shown that the more the ethylene producted the earlier the ripening period of fruit and the worse the storage characteristics. Since 1991, a lot of outstanding research results have been reached by the supporting of Grant-in-Aid for Scientific Research (B).The cultivars, which had a high content of ACC,the pre-producted substnace of ethylene, always had a high EFE activity. But, there were also some cultivars, such as Nijisseiki, which had higher ACC level and EFE activity but the ethylene production was quite limited.The relationship between the ethylene production and the activity of enzyme in vacuole membrane had been investigated, and the results shown there was no relation between then.In the late maturing cultivars, few ethylene production resulted from the lower activity of EFE and higher content of malonyl ACC which is a kind of polyamine related with putrescine.From 1989 to 1993,70 cultivars of Japanese pears were researched, and they were divided into 4 groups according to the result.

本文从1989年开始研究日本梨果实乙烯产生量与贮藏特性的关系，并与果实的成熟情况相联系。结果表明，乙烯释放量越大，果实成熟期越早，贮藏性越差。自1991年以来，在国家科学研究资助项目（B）的支持下，我国的一些品种取得了一批优秀的研究成果，乙烯前体物质ACC含量高的品种，其EFE活性也高。但也有一些品种，如二次成，其ACC水平和EFE活性较高，但乙烯产生量很有限，乙烯产生量与液泡膜酶活性之间的关系研究表明，乙烯产生量与液泡膜酶活性之间无相关性，晚熟品种中，乙烯产生量与液泡膜酶活性之间无相关性。1989 ~ 1993年，以70个日本梨品种为材料，根据结果分为4组。

项目成果

藪野美奈子・田辺賢二・田村文男・村上ゆり子・寿和夫: "ゲノムDNAのRFLP分析によるナシ属種の類別" 園芸学会雑誌. 62(別冊). 18-19 (1993)

Minako Yabuno、Kenji Tanabe、Fumio Tamura、Yuriko Murakami 和 Toshikazuo：“通过基因组 DNA 的 RFLP 分析对梨物种进行分类”，日本园艺学会杂志 62（分刊）。

田村文男・田辺賢二・伴野潔・池田隆政: "ニホンナシ‘二十世紀'の芽の休眠打破におよぼす高温処理の影響" 園芸学会雑誌. 62. 41-47 (1993)

Fumio Tamura、Kenji Tanabe、Kiyoshi Banno、Takamasa Ikeda：“高温处理对‘二十世纪’日本梨芽休眠的影响”日本园艺学会杂志 62. 41-47 (1993)。

Kenji Tanabe,H.Tamura and A.Itai: "Classification of Japanese Pear Cultivars by the Charactaristics of Ethylene Evolution in Ripening Fruits" 第24回国際園芸学会発表要旨. (印刷中). (1994)

Kenji Tanabe、H. Tamura 和 A. Itai：“根据成熟果实中乙烯进化的特征对日本梨品种进行分类”，第 24 届国际园艺学会摘要（1994 年）。

藪野美奈子・田辺賢二・田村文男・寿和夫: "DNAフィンガープリント法によるニホンナシ栽培品種の品種同定" 園芸学会雑誌. 62(別冊2). 178-179 (1993)

Minako Yabuno、Kenji Tanabe、Fumio Tamura、Kazuo Tamura：“利用 DNA 指纹识别日本梨品种”，日本园艺学会杂志 62（特刊 178-179）（1993 年）。

張 浚澤・田辺 賢二 田村 文男: "葉のペルオキシダーゼアイソザイム分析によるナシ族種の類別" 園芸学会雑誌. 61. 273-286 (1992)

张楚泽、田边健二、田村文雄：“通过叶过氧化物酶同工酶分析对梨物种进行分类”日本园艺学会杂志 61. 273-286 (1992)。