Origin and generation of verogenic Newcastle disease viruses

致新城疫病毒的起源和产生

• 批准号: 14360178
• 负责人: ITO Toshihiro
• 金额: $ 9.34万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14360178/
• 关键词: Newcastle disease; poultry; waterfowl; pathogenicity; origin; NDV; chicken; cleavage; HN; ノイラミダーゼ

A benign Newcastle disease virus(NDV) recently became highly virulent during replication in domestic chickens. It is still unclear whether NDVs circulating among wild waterfowl also have the potential to become highly pathogenic (velogenic) in chickens. To demonstrate experimentally the generation of velogenic NDV from a nonpathogenic waterfowl isolate, we passaged an avirulent goose isolate in chickens. After nine consecutive passages by air-sac inoculation, followed by five passages in chick brain, the virus became highly virulent in chickens, producing a 100% mortality rate, and demonstrating typical velogenic properties in pathogenicity tests. Sequence analysis at the fusion protein cleavage site showed that the original isolate contained the typical avirulent type sequence, E-R-Q-E-R/L, which progressed incrementally to a typical virulent type, K-R-Q-K-R/F, during repeated passage in chickens. These results demonstrate that avirulent viruses, maintained in wild waterfowl in nature and bearing the consensus avirulent type sequence, have the potential to become velogenic after transmission to and circulation in chicken populations. The results also suggest that chickens provide a mechanism for the selection of virulent viruses from an avirulent background.

一种良性的纽卡斯尔病病毒（NDV）在国内鸡群中复制过程中变得高度毒力。目前还不清楚在野生水禽中传播的NDV是否也有可能在鸡中成为高致病性（速发型）。为了证明非致病性水禽分离株能产生强毒NDV，我们将一株无毒鹅分离株在鸡体内传代。经气囊接种连续传9代，再经鸡脑传5代后，病毒对鸡的毒力增强，死亡率达100%，并表现出典型的速发型。在融合蛋白切割位点的序列分析表明，原始分离物含有典型的无毒型序列E-R-Q-E-R/L，在鸡中反复传代期间，该序列逐渐发展为典型的毒力型序列K-R-Q-K-R/F。这些结果表明，无毒病毒，保持在野生水禽的性质和轴承的共识无毒型序列，有可能成为传染后，在鸡群和循环的速度。结果还表明，鸡提供了一种机制，从无毒的背景选择强毒病毒。

项目成果

伊藤壽啓: "野生水禽由来ニューカッスル病のウイルスのニワトリへの侵入と病原性獲得機序"日本獣医学会家禽疾病学分科会報. 7. 2-7 (2003)

伊藤俊博：“来自野生水禽的新城疫病毒侵入鸡及其获得致病性的机制”日本兽医协会家禽疾病部门通报（2003年）。

Generation of velogenic Newcastle disease viruses from a nonpathogenic waterfowl isolate by passaging in chickens

• DOI: 10.1006/viro.2002.1539
• 发表时间: 2002-09-30
• 期刊: VIROLOGY
• 影响因子: 3.7
• 作者: Shengqing, Y;Kishida, N;Ito, T
• 通讯作者: Ito, T

Haemagglutininating activity of the lentogenic Newcastle disease virus strain MET95.

弱新城疫病毒株 MET95 的血凝活性。

• 发表时间: 2003
• 期刊: Avian Pathology 32
• 作者: Murakawa;Y.
• 通讯作者: Y.

Newcastle Disease Virus

• DOI: 10.1007/978-1-0716-2463-0_1093
• 发表时间: 2023-01-01
• 期刊: INFECTIOUS DISEASES, 2 EDITION
• 作者: Haddas, Ruth

獣医公衆衛生学第3版,ニューカッスル病

兽医公共卫生第三版，新城疫

• 发表时间: 2004
• 作者: 伊藤壽啓