Molecular signaling mechanisms underlying cardiovascular and branchial morphogenesis

心血管和鳃形态发生的分子信号机制

• 批准号: 14370231
• 负责人: KURIHARA Hiroki
• 金额: $ 9.54万
• 依托单位: The University of Tokyo (2003)Kumamoto University (2002)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370231/
• 关键词: neural crest cells; cardiovascular system; branchial arch; endothelin; gene-manipulated mouse; cell differentiation; transcription factor; morphogenesis; 心大血管形成; パターン形成; 分化制御; 血管平滑筋; 胚発生

1.Endothelin-1 (ET-1) has emerged as a key factor that regulates dorsoventral branchial arch patterning through the induction of Dlx5/6 homoebox transcription factors, thereby ET-1 appears to be involved in basic program of morphogenesis in this region.2.dHAND, a basic-helix-loop-helix transcription factor downstream to the ET-1 signaling, proved to be involved in the intermediate transition of vascular smooth muscle differentiation.3.Calpain6 has been identified as a factor downstream to the ET-1/dHAND pathway, which may participate in cell morphogenesis and proliferation by modification of cytoskeleton.4.TAZ has been identified as a transcriptional coactivator of Pax3, a Paired-type transcription factor involved in neural crest development.5.Transgenic mice in which EGFP and TVA (a receptor for avian retrovirus) expression are driven by the ETA receptor gene promoter has been established and served as a useful tool for researches on the cardiovascular system and neural crest development.

1.内皮素-1（endothelin-1，ET-1）是调控鳃弓背腹侧形态的关键因子，通过Dlx 5/6同源框转录因子的介导，参与了鳃弓背腹侧形态发生的基本过程。已证实参与血管平滑肌分化的中间过渡。3.钙蛋白酶6已被鉴定为ET-1/dHAND通路的下游因子，其可能通过修饰细胞因子参与细胞形态发生和增殖。4. TAZ已被鉴定为Pax 3的转录共激活因子，一种参与神经嵴发育的成对型转录因子。5.转基因小鼠，其中EGFP和TVA（禽逆转录病毒的受体）ETA受体基因启动子驱动的表达的研究已经建立，并作为心血管系统和神经嵴发育研究的有用工具。

项目成果

Yamaguchi Y: "Identification of multiple novel epididymis-specific -defensin isoforms in humans and mice"J.Immunol.. 169. 2516-2523 (2002)

Yamaguchi Y：“人类和小鼠中多种新型附睾特异性防御​​素亚型的鉴定”J.Immunol.. 169. 2516-2523 (2002)

Ito T: "Inhibitory effect of natriuretic peptides on aldosterone synthase gene expression in cultured neonatal rat cardiocytes."Circulation.. 107. 807-810 (2003)

Ito T：“利钠肽对培养的新生大鼠心肌细胞中醛固酮合酶基因表达的抑制作用。”Circulation.. 107. 807-810 (2003)

Nishimatsu H: "Role of endogenous adrenomedullin in the regulation of vascular tone and ischemic renal injury : studies on transgenic/knockout mice of adrenomedullin gene."Circ.Res.. 90. 657-663 (2002)

Nishimatsu H：“内源性肾上腺髓质素在调节血管张力和缺血性肾损伤中的作用：对肾上腺髓质素基因转基因/敲除小鼠的研究。”Circ.Res.. 90. 657-663 (2002)

Nishimatsu H: "Role of endogenous adrenomedullin in the regulation of vascular tone and ischemic renal injury : studies on transgenic/knockout mice of"Circ.Res.. 90. 657-663 (2002)

Nishimatsu H：“内源性肾上腺髓质素在调节血管张力和缺血性肾损伤中的作用：对转基因/基因敲除小鼠的研究”Circ.Res.. 90. 657-663 (2002)

Ieda M: "Endothelin-1 regulates cardiac sympathetic innervation in the rodent heart by controlling nerve growth factor expression."J.Clin.Invest.. 113. 876-884 (2004)

Ieda M：“Endothelin-1 通过控制神经生长因子的表达来调节啮齿类动物心脏的交感神经支配。”J.Clin.Invest.. 113. 876-884 (2004)