The mechanism in the diversity and amplification of mucosal immune system -Clinical application of mucosal vaccine-

1.针对流感嗜血杆菌（Hi）P6的免疫应答。将重组P6（rP 6）和纳米球偶联并鼻内施用至小鼠。尽管血清中P6特异性IgM和IgG水平升高，但在外分泌物中不诱导分泌性伊加，表明rP 6作为粘膜抗原的抗原性较低。2.粘液免疫应答针对磷酸胆碱（PC）。然而，PC的抗原性尚不清楚。当PC鼻内给药小鼠，诱导全身以及粘膜免疫反应，而不使用任何粘膜佐剂，如霍乱毒素。此外，PC诱导的抗体与几种不同的Hi菌株和肺炎链球菌有交叉反应。结果提示，PC可能是一种广谱的粘膜疫苗候选者。3. PC免疫抑制I型变态反应。然而，当PC鼻内或腹腔内给药小鼠，IgE的产生显着减少。此外，与对照组相比，从用PC免疫的小鼠中分离的脾辅助T细胞产生少得多的IL-4。这些结果表明，PC作为一种有前途的粘膜疫苗的有效性和安全性。

1.Immune responses against Haemophilus influenzae(Hi) P6.Recombinant P6(rP6) and nanosphere were coupled and intra-nasally administered to mice. Although P6-specific serum IgM and IgG levels were increased, secretory IgA was not induced in external secretions, indicating the low antigenesity of rP6 as mucosal antigen.2.Mucosal immune responses against phosphorylcholine(PC).PC is contained in all Gram-positive and -negative bacteria. However, the antigenesity of PC is not yet clarified. When PC was intra-nasally administered to mice, systemic as well as mucosal immune responses were induced without using any mucosal adjuvant such as cholera toxin. Moreover, the antibodies induced by PC were cross-reactive with several different strains of Hi and Streptococcus pneumoniae. Those results suggest that PC might be a candidate of mucosal vaccine with broad spectrum.3.Inhibition of type I allergy by immunization with PC.Mucosal vaccine frequently induces type I allergy by producing IgE. However, when PC was intra-nasally or intra-peritoneally administered to mice, the production of IgE was significantly reduced. Further, splenic helper T cells isolated from immunized mice with PC produced much less IL-4 compared with the controls. Those findings suggest the efficacy and the safety of PC as a promising mucosal vaccine.