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The Mechanisms of Mucosal Immune Responses in Adenoid and Tonsil (NALT)

腺样体和扁桃体 (NALT) 粘膜免疫反应的机制

基本信息

批准号：
09470374
负责人：
KURONO Yuichi
金额：
$ 2.5万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

Mucosal immune responses in adenoid tonsil1) Immune responses against Haemophilus influenzaeThe number of IgA-producing cells against H. influenzae was decreased in the samples of adenoid from which the bacteria was cultured. The same results were obtained in the study of tonsils. Those findings suggest that mucosal immune responses against bacteria are associated with the pathogenesis of upper respiratory diseases.2) The role of adenoidal epithelial cellsAdenoidal epithelial cells produced IL-8 and IL-1β in response to endotoxin purified from H. influenzae. Activation of NF-ィイD2κィエD2B was correlated with those cytokine production. The findings indicate that epithelial cells are also involved in the induction of mucosal immune responses.Mucosal immune responses in mice1) The effects of intranasal immunizationIntranasal, intratracheal, and oral immunizations with H. influenzae induced antigen-specific IgA producing cells in nasal mucosa and enhanced bacterial clearance from the nose. Those responses were most significant in intranasal immunization. Th1- as well as Th2-type cytokines were associated with the mucosal and systemic immune responses.2) Induction of immune toleranceIntranasal immunization with H. influenzae prior to systemic immunization did not suppress the production of IgG antibodies. However, when CD8ィイD1+ィエD1T cells activated by intranasal immunization were deleted, the number of IgG-producing cells was increased. The results indicate the capability of inducing immune tolerance by intranasal vaccination.

腺样体扁桃体中的粘膜免疫反应 1) 针对流感嗜血杆菌的免疫反应在培养细菌的腺样体样本中，针对流感嗜血杆菌的 IgA 产生细胞数量减少。对扁桃体的研究也得到了同样的结果。这些发现表明，针对细菌的粘膜免疫反应与上呼吸道疾病的发病机制有关。2）腺样上皮细胞的作用腺样上皮细胞响应从流感嗜血杆菌纯化的内毒素而产生IL-8和IL-1β。 NF-ィイD2κィエD2B 的激活与这些细胞因子的产生相关。研究结果表明，上皮细胞也参与诱导粘膜免疫反应。小鼠的粘膜免疫反应1）鼻内免疫的影响流感嗜血杆菌鼻内、气管内和口腔免疫诱导鼻粘膜中产生抗原特异性IgA的细胞，并增强了鼻子中的细菌清除率。这些反应在鼻内免疫中最为显着。 Th1型和Th2型细胞因子与粘膜和全身免疫反应相关。2)免疫耐受的诱导在全身免疫之前用流感嗜血杆菌鼻内免疫不会抑制IgG抗体的产生。然而，当鼻内免疫激活的CD8ィイD1+ィエD1T细胞被删除时，产生IgG的细胞数量增加。结果表明鼻内接种疫苗具有诱导免疫耐受的能力。