APPLICATION OF SUGAR ALCOHOLS COCKTAILS TO HUMAN CARIES PREVENTION

糖醇鸡尾酒在预防人类龋齿中的应用

基本信息

  • 批准号:
    14370687
  • 负责人:
  • 金额:
    $ 4.35万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2002
  • 资助国家:
    日本
  • 起止时间:
    2002 至 2003
  • 项目状态:
    已结题

项目摘要

Xylitol inhibits the glycolysis and growth of Streptococcus mutans. We studied the inhibitory effect of xylitol on the acid production of S.mutans at several pH levels under the strictly anaerobic conditions found in the deep layer of dental plaque. Xylitol inhibited the rate of acid production from glucose and changed the profile of acidic end products to formate-acetate dominance, with a decrease in the intracellular level of fructose 1,6-bisphosphate and an intracellular accumulation of xylitol 5-phosphate (X5P). These results were notable at pH 5.5-7.0, but were not evident at pH 5.0. Since the activity of phosphoenolpyruvate phosphotransferase for xylitol was greater at higher pH, it is suggested that xylitol could be incorporated more efficiently at higher pH and that the resultant accumulation of X5P could inhibit the glycolysis of S.mutans more effectively.In addtion, xylitol retarded the growth of mutans streptococci in the presence of glucose (G), galactose (Gal), maltose (M), lactose (L) or sucrose (5) as an energy source, though the inhibition of growth on fructose (Fr) was small. Xylitol inhibited acid production by washed cells of S.mutans from G,Gal,M,L or 5 (12-83% inhibition). S.mutans accumulated xylitol 5-phosphate (X5P) intracellularly through activity of the phosphoenolpyruvate-xylitol phosphotransferase system (PEP-xylitol PTS) when they fermented these sugars in the presence of xylitol. However, in the presence of Fr, no inhibition of acid production was observed. In addition, the amounts of X5P during the fermentationof Fr were smaller than those of other sugars in spite of the presence of PEP-xylitol PTS activity. These results suggest that along with the intracellular accumulation of XSP, xylitol decreases the growth and acid production of mutans streptococci in the presence of various dietary sugars except Fr.
木糖醇抑制变形链球菌的糖酵解和生长。在牙菌斑深层严格厌氧的条件下,研究了木糖醇在不同pH条件下对变形链球菌产酸的抑制作用。木糖醇抑制了葡萄糖的产酸速率,改变了酸性终产物的形态,使甲酸盐-醋酸酯占优势,胞内1,6-二磷酸果糖水平下降,木糖醇5-磷酸(X5P)积累。这些结果在pH 5.5-7.0时显著,但在pH 5.0时不明显。此外,木糖醇在葡萄糖(G)、半乳糖(Gal)、麦芽糖(M)、乳糖(L)或蔗糖(5)作为能源时,对变形链球菌的生长有抑制作用,但对变形链球菌的生长抑制作用较小。木糖醇对来自G、Gal、M、L或5的变形链球菌的洗涤细胞产酸有抑制作用(抑制率为12-83%)。变形链球菌在木糖醇存在下发酵5-磷酸木糖时,通过磷酸烯醇式丙酮酸-木糖醇磷酸转移酶系统(PEP-木糖醇PTS)的活性在细胞内积累木糖醇5-磷酸(X5P)。然而,在Fr存在的情况下,没有观察到产酸的抑制。此外,尽管存在PEP-木糖醇PTS活性,但Fr发酵过程中X5P的量比其他糖的量少。这些结果表明,随着XSP在细胞内的积累,木糖醇在除Fr外的各种饲料糖的存在下,降低了变形链球菌的生长和产酸。

项目成果

期刊论文数量(135)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kazuo Kato: "A method for mapping the distribution pattern of cariogenic streptococci within dental plaque in vivo"Caries Research. 38(in press). (2004)
Kazuo Kato:“绘制体内牙菌斑内致龋链球菌分布模式的方法”龋齿研究。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
角田初恵: "ミュータンスレンサ球菌の各種糖質における増殖と酸産生に及ぼすキシリトールの影響"小児歯誌. 40(2). 308 (2002)
Hatsue Tsunoda:“木糖醇对各种碳水化合物上变形链球菌生长和产酸的影响”《儿童牙科》40(2) 308 (2002)。
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    0
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菊地 吾郎: "一般医科学"南山堂. 391 (2002)
菊池五郎:《普通医学》Nanzando 391 (2002)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
Takuichi Sato: "Nested PCR for detection of mutans streptococci in dental plaque"Lett Appl Microbiol. 37(1). 66-69 (2003)
Takuichi Sato:“用于检测牙菌斑中变形链球菌的巢式 PCR”Lett Appl Microbiol。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
Hiroyuki Tada: "Proteolysis of ICAM-1 on human oral epithelial cells by cysteine proteinases (Gingipains) from Porphyromonas gingivalis leading to down-regulation of neutrophil adhesion"Journal of Dental Research. 82. B-41 (2003)
Hiroyuki Tada:“牙龈卟啉单胞菌的半胱氨酸蛋白酶(牙龈蛋白酶)对人口腔上皮细胞上的 ICAM-1 进行蛋白水解,导致中性粒细胞粘附下调”《牙科研究杂志》。
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    0
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TAKAHASHI Nobuhiro其他文献

A water-soluble glass-based temporary restorative resin inhibited bacteria-induced pH reductions at the bacteria-material interface
水溶性玻璃基临时修复树脂可抑制细菌引起的细菌-材料界面 pH 值降低
  • DOI:
    10.4012/dmj.2021-129
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    2.5
  • 作者:
    MAYANAGI Gen;YUFANG Luo;HOSHINO Tomohiro;TAKAHASHI Nobuhiro
  • 通讯作者:
    TAKAHASHI Nobuhiro
Effectiveness of an actuator-driven pulsed water jet for removal of softened carious dentin
执行器驱动的脉冲水射流去除软化龋齿牙本质的有效性
  • DOI:
    10.4012/dmj.2021-234
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    2.5
  • 作者:
    YOKOYAMA-SATO Yuka;NISHIOKA Takashi;NAGANUMA Yukihiro;TAKAHASHI Masatoshi;NAKAGAWA Atsuhiro;YODA Nobuhiro;SASAKI Keiichi;TAKAHASHI Nobuhiro;TOMINAGA Teiji;IIKUBO Masahiro
  • 通讯作者:
    IIKUBO Masahiro

TAKAHASHI Nobuhiro的其他文献

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{{ truncateString('TAKAHASHI Nobuhiro', 18)}}的其他基金

Metabolome analysis of oral biofilm: a possible risk assessment of oral diseases using oral microbiome metabolism
口腔生物膜的代谢组分析:利用口腔微生物组代谢对口腔疾病进行可能的风险评估
  • 批准号:
    26293439
  • 财政年份:
    2014
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Metabolomics of cancer tissue -challenge to the paradoxical energy metabolism of cancer cells
癌组织代谢组学——挑战癌细胞矛盾的能量代谢
  • 批准号:
    25670786
  • 财政年份:
    2013
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Metabolomic approach to metabolic biology of cancer cells
癌细胞代谢生物学的代谢组学方法
  • 批准号:
    23659866
  • 财政年份:
    2011
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Metabolomic approach to oral biofilm and saliva: possible risk indicator for oral diseases
口腔生物膜和唾液的代谢组学方法:口腔疾病的可能风险指标
  • 批准号:
    22390399
  • 财政年份:
    2010
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Research on the promotion of joint development in Japan
日本共同开发促进研究
  • 批准号:
    22530371
  • 财政年份:
    2010
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Metabolome analysis of oral biofilm using CE-TOFMS and its application possibility as an oral health indicator
CE-TOFMS 口腔生物膜代谢组分析及其作为口腔健康指标的应用可能性
  • 批准号:
    19390539
  • 财政年份:
    2007
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Profiling of oral bacterial flora using DNA chips and its application to the risk evaluation of oral diseases of children.
DNA芯片口腔菌群分析及其在儿童口腔疾病风险评估中的应用
  • 批准号:
    16390601
  • 财政年份:
    2004
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Focused proteomic analysis of functional network for peptidyl prolyl cis/trans isomerases
肽基脯氨酰顺/反异构酶功能网络的重点蛋白质组学分析
  • 批准号:
    15310139
  • 财政年份:
    2003
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
STUDY ON METABOLIC SYSTEMS FOR PEPTIDES AND AMINO ACIDS OF PERIODONTAL PATHOGENS, PORPHYROMONAS AND PREVOTELLA.
牙周病原菌、卟啉单胞菌和普氏菌肽和氨基酸代谢系统的研究。
  • 批准号:
    11470386
  • 财政年份:
    1999
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

相似海外基金

Prevention of Developmental Delay and Xylitol (PDDaX) Study
预防发育迟缓和木糖醇 (PDDaX) 研究
  • 批准号:
    10447994
  • 财政年份:
    2022
  • 资助金额:
    $ 4.35万
  • 项目类别:
Reduction of bloodstream infections from oral organisms in pediatric stem cell transplant: a randomized multicenter double-blind placebo-controlled study evaluating twice daily oral xylitol
减少儿科干细胞移植中口腔生物体的血流感染:一项随机多中心双盲安慰剂对照研究,评估每日两次口服木糖醇
  • 批准号:
    10688676
  • 财政年份:
    2022
  • 资助金额:
    $ 4.35万
  • 项目类别:
Prevention of Developmental Delay and Xylitol (PDDaX) Study
预防发育迟缓和木糖醇 (PDDaX) 研究
  • 批准号:
    10636847
  • 财政年份:
    2022
  • 资助金额:
    $ 4.35万
  • 项目类别:
Reduction of bloodstream infections from oral organisms in pediatric stem cell transplant: a randomized, multicenter, double-blind, placebo-controlled study evaluating twice daily oral xylitol
减少儿科干细胞移植中口腔生物体的血流感染:一项随机、多中心、双盲、安慰剂对照研究,评估每日两次口服木糖醇
  • 批准号:
    10176918
  • 财政年份:
    2021
  • 资助金额:
    $ 4.35万
  • 项目类别:
Development of functionalized carbon supported metal catalyst for one-pot conversion of xylose oligomers to xylitol
开发用于木糖低聚物一锅转化为木糖醇的功能化碳负载金属催化剂
  • 批准号:
    21K14461
  • 财政年份:
    2021
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
A new biotech process for xylitol production from ARCITEKBio Ltd.
ARCITEKBio Ltd. 生产木糖醇的新生物技术工艺。
  • 批准号:
    105111
  • 财政年份:
    2019
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Study
Supporting xylitol scale-up development
支持木糖醇规模化发展
  • 批准号:
    537791-2018
  • 财政年份:
    2019
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Experience Awards (previously Industrial Undergraduate Student Research Awards)
Co-production of astaxanthin and xylitol using Xanthophyllomyces dendrorhous from renewable resources
利用来自可再生资源的树状叶黄酵母联合生产虾青素和木糖醇
  • 批准号:
    16K06873
  • 财政年份:
    2016
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Does regular xylitol use reduce the number of acute otitis media episodes in young children? A pragmatic RCT
经常使用木糖醇是否可以减少幼儿急性中耳炎的发作次数?
  • 批准号:
    321025
  • 财政年份:
    2015
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Operating Grants
Genetically modified Trichoderma reesei strains for xylitol production
用于生产木糖醇的转基因里氏木霉菌株
  • 批准号:
    465427-2014
  • 财政年份:
    2014
  • 资助金额:
    $ 4.35万
  • 项目类别:
    Interaction Grants Program
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