Profiling of oral bacterial flora using DNA chips and its application to the risk evaluation of oral diseases of children.

据报道，口腔生物膜与口腔感染有关，如龋齿和牙周炎。此外，口腔生物膜是包含不同数量和种类的微生物的微生物生态系统，因此控制这些微生物区系被认为是预防这些口腔感染的方法之一。本研究的目的是建立利用16S核糖体RNA基因的物种特异性DNA探针检测口腔感染相关细菌的DNA微阵列系统。本研究选择了20株牙本质病变相关细菌包括变形链球菌和23株牙周炎相关细菌，包括牙龈卟啉单胞菌、连翘坦纳氏菌和脆弱嗜血杆菌，并检测了这些DNA微阵列的特异性和敏感性。此外，我们目前正计划利用100种牙菌斑生物膜，将DNA微阵列系统与直接和实时聚合酶链式反应的特异性和敏感性进行比较。

Oral biofilms have been reported to be associated with oral infections such as dental caries and periodontitis. And also, oral biofilms are microbial ecosystems which contain various amounts and sorts of microorganisms, and thus controlling these microbial florae are thought to be one of the ways toward prevention of these oral infections. The aim of this study was to establish DNA micro arrays system to detect oral infections-associated bacteria utilizing species-specific DNA probes of 16S ribosomal RNA genes. Twenty dentinal endodontic lesions-associated bacteria including mutans streptococci and twenty-three periodontitis-associated bacteria including Porphyromonas gingivalis, Tannerella forsythia and Mogibacterium timidum were selected in this study, and the specificity and sensitivity of the DNA micro arrays were tested. In addition, we have been planning to compare the DNA micro arrays system with the direct-＆ real-time PCR on their specificities and sensitivities utilizing one-hundred subgingival plaque biofilms for the present.