Profiling of oral bacterial flora using DNA chips and its application to the risk evaluation of oral diseases of children.

DNA芯片口腔菌群分析及其在儿童口腔疾病风险评估中的应用

• 批准号: 16390601
• 负责人: TAKAHASHI Nobuhiro
• 金额: $ 7.94万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390601/
• 关键词: DNA chips; 16S ribosomal RNA; DNA probe; DNA primers; Oral bacteria

Oral biofilms have been reported to be associated with oral infections such as dental caries and periodontitis. And also, oral biofilms are microbial ecosystems which contain various amounts and sorts of microorganisms, and thus controlling these microbial florae are thought to be one of the ways toward prevention of these oral infections. The aim of this study was to establish DNA micro arrays system to detect oral infections-associated bacteria utilizing species-specific DNA probes of 16S ribosomal RNA genes. Twenty dentinal endodontic lesions-associated bacteria including mutans streptococci and twenty-three periodontitis-associated bacteria including Porphyromonas gingivalis, Tannerella forsythia and Mogibacterium timidum were selected in this study, and the specificity and sensitivity of the DNA micro arrays were tested. In addition, we have been planning to compare the DNA micro arrays system with the direct-＆ real-time PCR on their specificities and sensitivities utilizing one-hundred subgingival plaque biofilms for the present.

据报道，口腔生物膜与口腔感染如龋齿和牙周炎有关。此外，口腔生物膜是含有各种数量和种类的微生物的微生物生态系统，因此控制这些微生物区系被认为是预防这些口腔感染的方法之一。本研究的目的是建立口腔感染相关细菌16S核糖体RNA基因种特异性探针的DNA微阵列检测系统。本研究选取20种牙本质病变相关细菌（包括变形链球菌）和23种牙周炎相关细菌（包括牙龈卟啉单胞菌、Tannerella和Mogibacterium timidum），检测DNA微阵列的特异性和敏感性。此外，我们一直在计划比较DNA微阵列系统与直接和实时PCR的特异性和敏感性，利用100龈下菌斑生物膜目前。

项目成果

Alkali-resistant bacteria in root canal systems.

• DOI: 10.1111/j.1399-302x.2004.00174.x
• 发表时间: 2004-12
• 期刊: Oral microbiology and immunology
• 作者: K. Nakajo;F. Nakazawa;M. Iwaku;E. Hoshino

Difference in the xylitol sensitivity of acid production among Streptococcus mutans strains, and its biochemical mechanism

变形链球菌菌株木糖醇产酸敏感性差异及其生化机制

• 发表时间: 2006
• 期刊: Oral Microbiol Immunol 21(4)
• 作者: Miyasawa-Hori H;Miyasawa-Hori H
• 通讯作者: Miyasawa-Hori H

Synergistic inhibition by combination of fluoride and xylitol on the glycolysis by mutans streptococci and its biochemical mechanism.

氟与木糖醇联用对变形链球菌糖酵解的协同抑制及其生化机制

• 发表时间: 2005
• 期刊: Caries Res 39(6)
• 作者: Ayako Yamada;Yuki Nakamura;Daigo Sugita;Shinya Shirosaki;Tadahiro Ohkuri;Hideo Katsukawa;Kazuaki Nonaka;Toshiaki Imoto;Yuzo Ninomiya;Haruaki Hayasaki et al.;Noriko MURAOKA et al.;Noboru Yamaguchi et al.;Ieyoshi Kobayashi et al.;Satoshi FUKUMOTO et al.;Yoshizawa S et al.;岡田祐佳 他;Shimizu K;Shimonishi M;Sato R;Shimonishi M;Sato R;Nakajo K;Miyasawa-Hori H;Mitani H;佐藤充太;Miyasawa-Hori H;Nakajo K;Washio J;Maehara H
• 通讯作者: Maehara H

口腔生化学 第4版

口腔生物化学第四版

• 发表时间: 2005
• 作者: 早川太郎;須田立雄;木崎治俊;畑隆一郎;高橋信博;宇田川信之
• 通讯作者: 宇田川信之

Detection frequency of periodontitis-associated bacteria by polymerase chain reaction in subgingival and supragingival plaque of periodontitis and healthy subjects

• DOI: 10.1111/j.1399-302x.2004.00172.x
• 发表时间: 2004-12-01
• 期刊: ORAL MICROBIOLOGY AND IMMUNOLOGY
• 作者: Mayanagi, G;Sato, T;Takahashi, N
• 通讯作者: Takahashi, N