Profiling of oral bacterial flora using DNA chips and its application to the risk evaluation of oral diseases of children.

据报道，口腔生物膜与口腔感染如龋齿和牙周炎有关。此外，口腔生物膜是含有各种数量和种类的微生物的微生物生态系统，因此控制这些微生物区系被认为是预防这些口腔感染的方法之一。本研究的目的是建立口腔感染相关细菌16S核糖体RNA基因种特异性探针的DNA微阵列检测系统。本研究选取20种牙本质病变相关细菌（包括变形链球菌）和23种牙周炎相关细菌（包括牙龈卟啉单胞菌、Tannerella和Mogibacterium timidum），检测DNA微阵列的特异性和敏感性。此外，我们一直在计划比较DNA微阵列系统与直接和实时PCR的特异性和敏感性，利用100龈下菌斑生物膜目前。

Oral biofilms have been reported to be associated with oral infections such as dental caries and periodontitis. And also, oral biofilms are microbial ecosystems which contain various amounts and sorts of microorganisms, and thus controlling these microbial florae are thought to be one of the ways toward prevention of these oral infections. The aim of this study was to establish DNA micro arrays system to detect oral infections-associated bacteria utilizing species-specific DNA probes of 16S ribosomal RNA genes. Twenty dentinal endodontic lesions-associated bacteria including mutans streptococci and twenty-three periodontitis-associated bacteria including Porphyromonas gingivalis, Tannerella forsythia and Mogibacterium timidum were selected in this study, and the specificity and sensitivity of the DNA micro arrays were tested. In addition, we have been planning to compare the DNA micro arrays system with the direct-＆ real-time PCR on their specificities and sensitivities utilizing one-hundred subgingival plaque biofilms for the present.