Profiling of oral bacterial flora using DNA chips and its application to the risk evaluation of oral diseases of children.

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Oral biofilms have been reported to be associated with oral infections such as dental caries and periodontitis. And also, oral biofilms are microbial ecosystems which contain various amounts and sorts of microorganisms, and thus controlling these microbial florae are thought to be one of the ways toward prevention of these oral infections. The aim of this study was to establish DNA micro arrays system to detect oral infections-associated bacteria utilizing species-specific DNA probes of 16S ribosomal RNA genes. Twenty dentinal endodontic lesions-associated bacteria including mutans streptococci and twenty-three periodontitis-associated bacteria including Porphyromonas gingivalis, Tannerella forsythia and Mogibacterium timidum were selected in this study, and the specificity and sensitivity of the DNA micro arrays were tested. In addition, we have been planning to compare the DNA micro arrays system with the direct-＆ real-time PCR on their specificities and sensitivities utilizing one-hundred subgingival plaque biofilms for the present.